Rotational and lateral diffusion of membrane proteins

Cherry, Richard J.

doi:10.1016/0304-4157(79)90009-1

Cited by 451 publications

(215 citation statements)

References 133 publications

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“…We note that the rotational correlation time obtained from STEPR is only an approximate value; it is based on the calibration curve derived from the isotropic motion of spin-label. The values obtained, however, agree with those obtained by other methods, such as flash photolysis (32). Moreover, although our main interest in this study was the relative τ 2 of spin-labeled cytochrome caa 3 in the absence and presence of the F 1 F 0 -synthase from alkaliphilic B. pseudofirmus OF4, the τ 2 values obtained were in agreement with the DSC data (i.e.…”

Section: Stepr Studies Of Spin-labeled Cytochrome Caa 3 Embedded In Psupporting

confidence: 89%

Interaction between Cytochrome caa₃ and F₁F₀-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Gong

Hicks

et al. 2007

Biochemistry

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Interaction between the cytochrome caa 3 respiratory chain complex and F 1 F 0 -ATP synthase from extremely alkaliphilic Bacillus pseudofirmus OF4 has been hypothesized to be required for robust ATP synthesis by this alkaliphile under conditions of very low protonmotive force. Here, such an interaction was probed by differential scanning calorimetry (DSC) and by saturation transfer electron paramagnetic resonance (STEPR). When the two purified complexes were embedded in phospholipids vesicles individually [(caa 3 ) × PL, (F 1 F 0 ) × PL)] or in combination [(caa 3 +F 1 F 0 ) × PL] and subjected to DSC analysis, they underwent exothermic thermodenaturation with transition temperatures at 69, 57, and 46/75 °C, respectively. The enthalpy change, ΔH, (−8.8 Kcal/mmol) of protein-phospholipid vesicles containing both cytochrome caa 3 and F 1 F 0 was smaller than that (−12.4 Kcal/mmol) of a mixture of protein-phospholipid vesicles formed from each individual electron transfer complex [(caa 3 × PL) + (F 1 F 0 × PL)]. The rotational correlation time of spin-labeled caa 3 (65 µs) in STEPR studies increased significantly when the complex was mixed with F 1 F 0 prior to being embedded in phospholipids vesicles (270 µs). When the complexes were reconstituted separately then mixed together, or either mitochondrial cytochrome bc 1 or F 1 F 0 was substituted for the alkaliphile F 1 F 0 , the correlation time was unchanged (65-70 µs). Varying the ratio of the two alkaliphile complexes in both the DSC and STEPR experiments indicated that the optimal stoichiometry is 1:1. These results demonstrate a specific interaction between the cytochrome caa 3 and F 1 F 0 -ATP synthase from B. pseudofirmus OF4 in a reconstituted system. They support the suggestion that physical association between these complexes may contribute to sequestered proton transfers during alkaliphile oxidative phosphorylation at high pH.

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Section: Stepr Studies Of Spin-labeled Cytochrome Caa 3 Embedded In Psupporting

confidence: 89%

Interaction between Cytochrome caa₃ and F₁F₀-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Gong

Hicks

et al. 2007

Biochemistry

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“…3 show that the probability that the observed differences between the cell body and axon hillock and cell body and neuritic terminal are significant is >99.7%. The diffusion coefficients for channels measured at each region are in the range reported for other intrinsic membrane proteins (13), although sodium channels on the cell body are at the upper end of the range for cell surface proteins measured by FPR.…”

Section: Lateral Mobility Of Voltage-dependent Sodium Channels On Nervementioning

confidence: 65%

“…Measurements of the diffusion of the lipid probe TmRhd-PE or DiI, however, revealed no regional differences on spinal cord neurons (Tables II and III). The fluorescent phospholipid derivatives diffused freely in all membrane regions examined with a high diffusion coefficient of 5-7 × 10 -9 cm2/s, characteristic of these lipid probes (13,45,46). Hence inhomogeneities in the membrane lipid viscosity cannot account for the differences in channel diffusion rates observed in different regions of the neurons, and any barriers that restrict sodium channel diffusion from the cell body to the axon hillock do not influence diffusion of lipids.…”

Section: Lateral Mobility Of Voltage-dependent Sodium Channels On Nervementioning

confidence: 95%

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Distribution and lateral mobility of voltage-dependent sodium channels in neurons [published erratum appears in J Cell Biol 1989 May;108(5):preceding 2001]

Angelides

Elmer

Loftus

et al. 1988

The Journal of Cell Biology

146

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Abstract. Voltage-dependent sodium channels are distributed nonuniformly over the surface of nerve cells and are localized to morphologically distinct regions. Fluorescent neurotoxin probes specific for the voltagedependent sodium channel stain the axon hillock 5-10 times more intensely than the cell body and show punctate fluorescence confined to the axon hillock which can be compared with the more diffuse and uniform labeling in the cell body. Using fluorescence photobleaching recovery (FPR) we measured the lateral mobility of voltage-dependent sodium channels over specific regions of the neuron. Nearly all sodium channels labeled with specific neurotoxins are free to diffuse within the cell body with lateral diffusion coefficients on the order of 10 -9 cm2/s. In contrast, lateral diffusion of sodium channels in the axon hillock is restricted, apparently in two different ways. Not only do sodium channels in these regions diffuse more slowly (10-1°-10 -H cm2/s), but also they are prevented from diffusing between axon hillock and cell body. No regionalization or differential mobilities were observed, however, for either tetramethylrhodaminephosphatidylethanolamine, a probe of lipid diffusion, or FITC-succinyl concanavalin A, a probe for glycoproteins. During the maturation of the neuron, the plasma membrane differentiates and segregates voltagedependent sodium channels into local compartments and maintains this localization perhaps either by direct cytoskeletal attachments or by a selective barrier to channel diffusion.

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“…The protein concentration of the OM preparations varied between 1 and 6 mg/ml. The instrumentation for spectroscopy and data treatment has been described in detail [8,9]. Briefly, rotation was measured by observing transient dichroism of ground-state depletion signals arising from excitation of the eosin probe by a linearly polarized light pulse from a NA-YAG laser (J.K.…”

Section: Cell Membrane Prewrationmentioning

confidence: 99%

Measurement of the rotational behaviour of virus particles during adsorption to the host cell surface

Bayer¹,

Morrison

Cherry

1984

FEBS Letters

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The rotational diffusion of bacteriophage ~15 was measured before and after virus adsorption to outer membrane vesicles of the host Salmonella anatzun. The virus capsid was labeled with eosin isothiocyanate, and the decay of transient dichroism following dye excitation by pulses of plane-polarized light was measured. From the data, the rotational diffusion constant of the unadsorbed virion and its hydrodynamic diameter were estimated and found to be consistent with electron microscopic measurements of the capsid dimensions. Addition of outer membrane vesicles of 5'. anatum to the virus suspension revealed the immobilization of the virus particles on the membrane surface.

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Rotational and lateral diffusion of membrane proteins

Cited by 451 publications

References 133 publications

Interaction between Cytochrome caa₃ and F₁F₀-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Interaction between Cytochrome caa₃ and F₁F₀-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Distribution and lateral mobility of voltage-dependent sodium channels in neurons [published erratum appears in J Cell Biol 1989 May;108(5):preceding 2001]

Measurement of the rotational behaviour of virus particles during adsorption to the host cell surface

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Rotational and lateral diffusion of membrane proteins

Cited by 451 publications

References 133 publications

Interaction between Cytochrome caa3 and F1F0-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Interaction between Cytochrome caa3 and F1F0-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Distribution and lateral mobility of voltage-dependent sodium channels in neurons [published erratum appears in J Cell Biol 1989 May;108(5):preceding 2001]

Measurement of the rotational behaviour of virus particles during adsorption to the host cell surface

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Interaction between Cytochrome caa₃ and F₁F₀-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays

Interaction between Cytochrome caa₃ and F₁F₀-ATP Synthase of Alkaliphilic Bacillus pseudofirmus OF4 Is Demonstrated by Saturation Transfer Electron Paramagnetic Resonance and Differential Scanning Calorimetry Assays