Abstract:The rotational diffusion of bacteriophage ~15 was measured before and after virus adsorption to outer membrane vesicles of the host Salmonella anatzun. The virus capsid was labeled with eosin isothiocyanate, and the decay of transient dichroism following dye excitation by pulses of plane-polarized light was measured. From the data, the rotational diffusion constant of the unadsorbed virion and its hydrodynamic diameter were estimated and found to be consistent with electron microscopic measurements of the caps… Show more
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