Rotating drum fermentor for plant cell suspension cultures

Tanaka, Hideo; Nishijima, Fuyuhiko; Suwa, Mitsuru; Iwamoto, Takeaki

doi:10.1002/bit.260251007

Cited by 75 publications

(25 citation statements)

References 8 publications

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“…In comparison to microbes, plant cells grow very slowly with maximum growth rates typically [33] corresponding to a doubling time of about 20h. The fast growing cell lines have minimum doubling times of 30-60h, while slow-growing lines have doubling times in the region of 100h or more ( Table 2).Such low growth rates translate into long fermentation times which makes the large scale culturing of such cells potentially expensive and precautions preventing contamination very important.…”

Section: Growth Rates and Biomass Yieldsmentioning

confidence: 99%

The scale-up of plant cell culture: Engineering considerations

Taticek

Moo‐Young

Legge

1991

Plant Cell Tiss Organ Cult

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The enormous versatility of plants has continued to provide the impetus for the development of plant tissue culture as a commercial production strategy for secondary metabolites. Unfortunately problems with slow growth rates and low products yields, which are generally non-growth associated and intracellular, have made plant cell culture-based processes, with a few exceptions, economically unrealistic. Recent developments in reactor design and control, elicitor technology, molecular biology, and consumer demand for natural products, are fuelling a renaissance in plant cell culture as a production strategy. In this review we address the engineering consequences of the unique characteristics of plant cells on the scale-up of plant cell culture.

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Section: Growth Rates and Biomass Yieldsmentioning

confidence: 99%

The scale-up of plant cell culture: Engineering considerations

Taticek

Moo‐Young

Legge

1991

Plant Cell Tiss Organ Cult

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“…Likewise, cell suspensions in liquid medium are preferable to calli grown on solid medium because they can be used to generate large-batch continuous cultures with existing fermentation technology [e.g. 3,25]. However, fine control of somatic embryogenesis, clonal fidelity and culture quality will be an absolute prerequisite to future industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Influence of dicamba and casein hydrolysate on somatic embryo number and culture quality in cell suspensions of Dactylis glomerata (Gramineae)

Gray

Conger

1985

Plant Cell Tiss Organ Cult

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The effects of various concentrations and combinations of dicamba (3,6-diehloro-o-anisic acid) and casein hydrolysate on growth, mucilage accumulation, somatic embryo and root development in suspension cultures ofDactylis glomerata L. (orchardgrass) were examined. Fresh weight of culture tissue was increased with 20/zM but not with 80 or 160~M dicamba in treatments with 1-4g/1 casein hydrolysate. Different casein hydrolysate concentrations did not alter the amount of mucilage (measured by viscosity) in the supernatant in the absence of dicamba. However, the addition of dicamba increased viscosity with 80~aM giving the maximum response. Casein hydrolysate produced the greatest viscosity at 1-3 g/1 in treatments where dicamba was present. Both dicamba and casein hydrolysate were required for development of somatic embryos. Dicamba at 40/zM with 3-4g/1 casein hydrolysate produced approximately 2000 embryos/35 ml of suspension. Root development was inhibited by dieamba and stimulated by the presence of casein hydrolysate. The usefulness of medium component manipulations for influencing somatic embryogenesis and culture quality is discussed.

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“…Tanaka et al (1983), Shibasaki et al (1992), and Takahashis and Fujita (1991) successfully used a rotating drum bioreactor for the cultivation of suspension cultures of Catharanthus roseus, Nicotiana tabacum, and Lithospermum erythrorhizon. In comparison to the other bioreactor types described earlier, the rotating drum bioreactor has a significantly larger surface area in relation to volume.…”

Section: Suitable Bioreactors For Plant Cell Suspension Culturesmentioning

confidence: 98%

Plant Cell-Based Bioprocessing

Eibl

2009

Cell and Tissue Reaction Engineering

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Plant cell-based bioprocessing is the use of plant cell and tissue cultures for the production of biologically active substances (low molecular secondary metabolites and recombinant proteins). The most significant advantage of plant cell culture over the traditionally grown whole wild plant or engineered transgenic plant is the sterile production of metabolites under defined controlled conditions independent of climatic changes and soil conditions, which means that variations in product yield and quality can be better avoided. Furthermore, regulatory requirements such as the cGMP standards, which have to be adhered to in the early stages of pharmaceutical production, are more easily met.Moreover, plant cells are capable of performing complex posttranslational processing, which is a precondition for heterologous protein expression. When compared with mammalian cells, which currently dominate in the commercial protein manufacture, plant cell cultures as alternative expression systems guarantee safer processes because there is a lower risk of contamination by mammalian viruses, pathogens, and toxins. In addition to this considerable advantage, the process costs can also be substantially reduced. This is due to the fact that plant cell culture medium is very simple in composition and therefore relatively inexpensive.This chapter provides an overview of culture types, techniques, and suitable bioreactors used to produce secondary metabolites and recombinant proteins in plant cells. We describe plant cell culture basics, discuss key topics relevant to plant cell bioreactor engineering with application examples, and give an overview of approaches to improving productivity of plant cell-based processes.

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Rotating drum fermentor for plant cell suspension cultures

Cited by 75 publications

References 8 publications

The scale-up of plant cell culture: Engineering considerations

The scale-up of plant cell culture: Engineering considerations

Influence of dicamba and casein hydrolysate on somatic embryo number and culture quality in cell suspensions of Dactylis glomerata (Gramineae)

Plant Cell-Based Bioprocessing

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