Rosmarinic acid improves function and in vitro fertilising ability of boar sperm after cryopreservation

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

“…and apeginin-7-glucoside (1.113 mg/g extract) that have scavenging antioxidant activity against free radicals interpret the benefit beyond the enrichment of rabbit semen tris-based extender to overcome the lipid peroxidation process induced via chilling period [54][55][56][57][58][59]. This elongates the period of chilling in rabbit extended semen above 72 h in agreement with Di Lorio et al [9] and Johinke et al [10].…”

Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes

“…The addition of antioxidant substances to the freezing extender enhances the survival of sperm (Alvarez and Storey, 1995). In a previous study we found that 105 µM RA preserved sperm motility and quality at 120 min post-thawing due to the scavenging activity of RA (Luño et al, 2014). In this study, sperm motility and membrane integrity were significantly higher after thawing in semen samples treated with RA as compared to the control.…”

“…In this study, sperm motility and membrane integrity were significantly higher after thawing in semen samples treated with RA as compared to the control. The in vitro fertilising capacity of sperm samples treated with RA was also higher than that of the control sperm (Luño et al, 2014). However, for unknown reasons, RA supplementation did not enhance in vivo fertilisation rates after AI.…”

“…Studies on the incorporation of antioxidant compounds to improve the quality of FT semen have been extensive (Kaeoket et al, 2010;Malo et al, 2010;Malo et al, 2012), although the effect of antioxidant supplementation on sow fertility after AI is only marginally known. Antioxidant compounds from plant extracts, such as rosmarinic acid (RA), ameliorate the deleterious effects produced by oxidative stress during cryopreservation and improve sperm quality and in vitro fertilisation parameters when they are included in freezing extender solutions (Luño et al, 2014).…”

Artificial insemination in pigs today

“…In these facilities, boars are housed in pens or stalls with anywhere from 25 up to 2000 boars housed at an individual site [1,20,[33][34][35]. The facilities are designed to control the environment and management systems to optimize health and fertility of the sires [36][37][38]. Depending on their size, individual studs often serve as a supplier to 20 to 200 farms with production of hundreds to thousands of doses of semen each week.…”