Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes

Luño, Victoria; Gil, L.; Olaciregui, Maite; Grandía, Juan; Ansó, Trinidad; Blas, Ignacio de

doi:10.1556/avet.2015.008

Cited by 4 publications

(5 citation statements)

References 30 publications

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“…Deep intrauterine insemination with reduced number of sperm (1,000 x 10 6 ) 49 77.55 9.31 ± 0.41 Roca, Carvajal, Lucas, Vázquez, and Martínez (2003) Freezing medium supplemented with caffeine and CaCl 2 21 71.4 8.2 ± 0.9 Yamaguchi, Funahashi, and Murakami (2009) Freezing medium supplemented with cholesterol-loaded cyclodextrins 34 52.9 11.3 ± 0.9 Tomás, Blanch, Cebrián, and Mocé (2013) Addition of oxytocin to the thawed sperm suspension immediately before AI 48 88.8 8.4 ± 0.5 Okazaki et al (2014) Freezing medium supplemented with rosmarinic acid 10 55.3 9.7 ± 0.4 Luño et al (2015) Freezing medium supplemented with 6.0 mg/ ml of alpha-lipoic acid 20 75 10.1 ± 1.1 Shen, Jiang, Li, Hu, and Li (2016) pig breeds: Duroc, Large White and Landrace (Ma et al, 2013).…”

Section: No Of Young Per Litter Referencementioning

confidence: 99%

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

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Contents In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection. In both methods and due to the sensitivity of boar sperm to osmotic and temperature changes, there is a loss in the quality of the initial sample; however, slow freezing in boar semen has greater deleterious effects on the sample that are reflected in the pregnancy rates and number of live births. Therefore, only 1% of all inseminations are done with frozen semen. The aim of this review is to provide advances and results of studies conducted on the preservation of boar semen, delving more deeply into the critical points that each of the preservation techniques presents, including bacterial contamination, extender components, temperature, ice nucleation, use of additives in extenders and the main deleterious effects on sperm quality.

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Section: No Of Young Per Litter Referencementioning

confidence: 99%

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

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show abstract

“…) have only minimal effects on in vivo fertility (Luño et al. ). Similarly, not all sperm additives, such as caffeine (Yamaguchi et al.…”

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

“…Excellent fertility (>90% farrowing rate and 13.0 total born) was obtained with glutathione addition when using a total of three billion frozenthawed sperm in a double IUI. However, not all antioxidants have worked as well and might be attributed to the concentration and the freezing conditions (Buranaamnuay et al 2011a), or perhaps while improv-ing in vitro sperm motility and viability and IVF (Luño et al 2014) have only minimal effects on in vivo fertility (Luño et al 2015). Similarly, not all sperm additives, such as caffeine (Yamaguchi et al 2009) or prostaglandin (Knox and Yantis 2014) have worked well to improve fertility when used with AI.…”

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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Contents One of the limits to practical use of frozen boar sperm involves the lowered fertility when used for artificial insemination. Years of studies have shown that 5–6 billion sperm (approximately 3 billion viable) used in single or multiple inseminations results in pregnancy rates most often between 60 and 70% and with litter sizes between nine and 10 pigs. Yet today, it is not uncommon for studies to report pregnancy rates from 70 to 85% and litter sizes with 11–12 pigs. While global statements about the incidence and reasons for higher fertility are not conclusive, incremental fertility improvements appear independently associated with use of a minimum number of viable sperm (1–2 billion), insemination timing that increases the probability that sperm will be present close to ovulation for groups of females, selection for boar sperm survival following cryopreservation, and modification of the freeze and thaw conditions using additives to protect sperm from oxidative damage. Studies show that techniques such as intrauterine and deep uterine insemination can provide an opportunity to reduce sperm numbers and that control of time of ovulation in groups of females can reduce the need for multiple inseminations and improve the chance for AI close to ovulation. However, optimal and consistent fertility with cryopreserved boar sperm may require a multifaceted approach that includes boar selection and screening, strategic use of additives during the freezing and thawing process, post‐thaw evaluation of sperm and adjustments in sperm numbers for AI, assessment of female fertility and ovulation induction for single insemination. These sequenced procedures should be developed and incorporated into a quality control system for improved fertility when using minimal numbers of cryopreserved boar sperm.

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“…In a different approach, considering the effect of ambient temperature, simultaneously observing the temperature change of vulva and other body parts (gluteal area, udder, and ear base), or the temperature difference change between these body parts and vulvar skin temperature. The results showed that changes in vulvar skin temperature were the most correlated with estrus and that the mean temperature of vulvar skin of gilts and multiparous sows increased at the onset of estrus and decreased before ovulation [3,[23][24][25] . Moreover, the validity of vulvar skin average temperature variation as a predictor of sow ovulation was evaluated by inseminating sows once when the vulvar skin temperature of diestrous sows was 20% increased [1] or below 35°C [26] , where the threshold of 35°C resulted from Luño et al [18] The results showed comparable reproductive performance with the multiple insemination strategy of the conventional back-pressure test.…”

Section: Introductionmentioning

confidence: 99%

Automatic detection of sow estrus using a lightweight real-time detector and thermal images

Zheng,

Zhang,

Song

et al. 2023

International Journal of Agricultural and Biological Engineering

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Determination of ovulation time is one of the most important tasks in sow reproduction management. Temperature variation in the vulva of the sows can be used as a predictor of ovulation time. However, the skin temperatures of sows in existing studies are obtained manually from infrared thermal images, posing an obstacle to the automatic prediction of ovulation time. In this study, an improved YOLO-V5s detector based on feature fusion and dilated convolution (FD-YOLOV5s) was proposed for the automatic extraction of the vulva temperature of sows based on infrared thermal images. For the purpose of reducing the model complexity, the depthwise separable convolution and the modified lightweight ShuffleNet-V2 module were introduced in the backbone. Meanwhile, the feature fusion network structure of the model was simplified for efficiency, and a mixed dilated convolutional module was designed to obtain global features. The experimental results show that FD-YOLOV5s outperformed the other nine methods, with a mean average precision (mAP) of 99.1%, an average frame rate of 156.25 fps, and a model size of only 3.86 MB, indicating that the method effectively simplifies the model while ensuring detection accuracy. Using a linear regression between manual extraction and the results extracted using this method in randomly selected thermal images, the coefficients of determination for maximum and average vulvar temperatures reached 99.5% and 99.3%, respectively. The continuous vulva temperature of sows was obtained by the target detection algorithm, and the sow estrus detection was performed by the temperature trend and compared with the manually detected estrus results. The results showed that the sensitivity, specificity, and error rate of the estrus detection algorithm were 89.3%, 94.5%, and 5.8%, respectively. The method achieves real-time and accurate extraction of sow vulva temperature and can be used for the automatic detection of sow estrus, which could be helpful for the automatic prediction of ovulation time.

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Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes

Cited by 4 publications

References 30 publications

Preservation of boar semen: An update

Preservation of boar semen: An update

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Automatic detection of sow estrus using a lightweight real-time detector and thermal images

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