Roseovarius halotolerans sp. nov., isolated from deep seawater

Oh, Yong-Sik; Lim, Hayung-Joon; Cha, In-Tae; Im, Wan‐Taek; Yoo, Jae-Soo; Kang, Ui Gum; Rhee, Sung‐Keun; Roh, Dong-Hyun

doi:10.1099/ijs.0.002576-0

Cited by 42 publications

(22 citation statements)

References 23 publications

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“…Subsequently, nine further Roseovarius species with validly published names, Roseovarius nubinhibens (González et al 2003), Roseovarius crassostreae (Boettcher et al 2005), Roseovarius mucosus (Biebl et al 2005), Roseovarius aestuarii (Yoon et al 2008), Roseovarius pacificus (Wang et al 2009(Wang et al , 2010, Roseovarius halotolerans (Oh et al 2009), Roseovarius nanhaiticus (Wang et al 2010), Roseovarius marinus (Jung et al 2011) and Roseovarius indicus (Lai et al 2011), have been described from a hypersaline lake, seawater, a dinoflagellate culture, oysters and marine sediments (Labrenz et al 1999;González et al 2003;Biebl et al 2005;Boettcher et al 2005;Yoon et al 2008;Wang et al 2009Wang et al , 2010. In this study, we describe a bacterial strain, designated GSW-M15 T , which was isolated from seawater on the southern coast of South Korea and found to be phylogenetically affiliated to the genera Roseovarius and Donghicola on the basis of comparative 16S rRNA gene sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Jung

Park

Yoon

2012

Antonie van Leeuwenhoek

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Section: Introductionmentioning

confidence: 99%

Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Jung

Park

Yoon

2012

Antonie van Leeuwenhoek

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“…The genus Roseovarius accommodates catalase-positive, ovoid or rodshaped, obligately aerobic bacteria and, to date, all Roseovarius type strains have been retrieved from marine environments such as tidal flats, seawater, sea squirts and oysters. At the time of writing, the genus Roseovarius included 13 species with validly published names: R. tolerans (Labrenz et al, 1999), R. nubinhibens (González et al, 2003), R. mucosus (Biebl et al, 2005), R. crassostreae (Boettcher et al, 2005), R. aestuarii (Yoon et al, 2008), R. halotolerans (Oh et al, 2009), R. pacificus (Wang et al, 2009), R. nanhaiticus (Wang et al, 2010), R. indicus (Lai et al, 2011), R. marinus (Jung et al, 2011), R. halocynthiae (Kim et al, 2012b), R. litoreus (Jung et al, 2012) and R. sediminilitoris (Park & Yoon, 2013). Tidal flats, called getbol in Korean, are broad, low-gradient, coastal muddy marshes that play important roles in habitat restoration and nutrient recycling (Winberg et al, 2007;Math et al, 2012).…”

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confidence: 99%

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

Choi

Lee

Kim

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain 112 T , was isolated from a tidal flat at Taean, Korea. Cells were catalase-and oxidase-positive ovoids to rods and did not produce bacteriochlorophyll a. Optimum growth of strain 112T was observed at 30 6C, at pH 6.5-7.5 and in the presence of 2-4 % (w/v) NaCl.The major cellular fatty acids were summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) and C 16 : 0 and Q-10 was detected as the predominant ubiquinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and three unidentified lipids. Math et al., 2012). In this study, we describe the taxonomic characterization of a novelRoseovarius strain, designated 112 T , isolated from a tidal flat of the Yellow Sea in South Korea. Strain 112T was isolated from a surface tidal flat (less than 5 cm depth) in the Taean area (36 u 489 50.820 N 126 u 119 09.560 E), using a previously described procedure (Jin et al., 2011) with some modifications. Briefly, the tidal flat sample was serially diluted with artificial seawater (ASW; Jeong et al., 2013), spread on marine agar 2216 (MA; BD) and incubated at 25 u C for 3 days. The 16S rRNA genes of isolates that grew on MA were amplified by PCR using the universal primers F1 and R13 (Jin et al., 2011). The amplicons were double-digested with HaeIII and HhaI and the restriction fragment patterns were used as a guide to classify the colonies, as described previously (Jin et al., 2012). Based on restriction fragment patterns, PCR products with unique patterns were selected and sequenced using the F1 primer. The resulting 16S rRNA gene sequences were analysed using the Nucleotide Similarity Search program (http://eztaxon-e.ezbiocloud.net/; Kim et al., 2012a). From this analysis, a novel strain belonging to the genus Roseovarius, designated strain 112 T , was selected for further phenotypic and phylogenetic analysis. Strain 112T was routinely grown aerobically on MA at 30 u C for 2 days, except where indicated otherwise. Strain 112T was stored at 280 u C in marine broth (MB; BD) supplemented with 10 % (v/v) glycerol. The type strains R. mucosus KACC 12996 T , R. tolerans KACC 13027 T and R. nanhaiticus KACC 12561T were used as reference strains for phenotypic comparisons and fatty acid analysis.Abbreviation: BChl, bacteriochlorophyll.

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“…Eight further Roseovarius species, Roseovarius nubinhibens (González et al, 2003), R. crassostreae (Boettcher et al, 2005), R. mucosus (Biebl et al, 2005), R. aestuarii (Yoon et al, 2008), R. pacificus (Wang et al, 2009), R. halotolerans (Oh et al, 2009), R. nanhaiticus (Wang et al, 2010) and R. marinus (Jung et al, 2011), have since been described. Members of the genus Roseovarius have been isolated from a hypersaline lake, seawater, a dinoflagellate culture, oysters and marine sediment (Labrenz et al, 1999;González et al, 2003;Biebl et al, 2005;Boettcher et al, 2005;Yoon et al, 2008;Wang et al, 2009).…”

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confidence: 99%

Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi

Kim

Kong

Park

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, motile, ovoid-to rod-shaped bacterial strain, designated MA1-10 T , was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. Strain T grew optimally at pH 7.0-8.0, at 30 6C and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain MA1-10 T clustered with Roseovarius crassostreae CV919-312 T , with which it exhibited 97.1 % sequence similarity, at a bootstrap resampling value of 96.2 %. It exhibited 93.3-95.8 % 16S rRNA gene sequence similarity to the type strains of other recognized Roseovarius species. Strain MA1-10 T contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c as the major fatty acid, which is consistent with data for the genus Roseovarius. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid. The DNA G+C content of strain MA1-10 T was 55.4 mol%. Mean DNA-DNA relatedness between strain MA1-10 T and R. crassostreae DSM 16950 T was 13 %.Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain MA1-10 T could be distinguished from all recognized Roseovarius species. On the basis of the data presented, strain MA1-10

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Roseovarius halotolerans sp. nov., isolated from deep seawater

Cited by 42 publications

References 23 publications

Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi

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