Roseomonas aerophila sp. nov., isolated from air

Abstract

An aerobic, Gram-stain-negative, motile, mesophilic bacterium, designated strain 7515T-07 T , was isolated from an air sample in the Taean region, Republic of Korea. The strain grew at 4-40 6C (optimum, 30 6C) and pH 5.0-9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 7515T-07 T was related to members of the genus Roseomonas and had highest 16S rRNA gene sequence similarity with Roseomonas ludipueritiae 170/96 T (96.7 %). 16S RNA gene sequence similarity between strain 7515T-07 T and Roseomonas gilardii ATCC 49956 T (the type species of the genus Roseomonas) was 93.4 %. Strain 7515T-07 T contained Q-10 as the ubiquinone and C 18 : 1 v7c and C 19 : 0 cyclo v8c as the dominant fatty acids (.10 %). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified aminolipids. The DNA G+C content was 73.0 mol%. Combined data from phenotypic, phylogenetic and chemotaxonomic studies demonstrated that strain 7515T-07 T is a representative of a novel species of the genus Roseomonas, for which the name Roseomonas aerophila sp. nov. is proposed. The type strain is 7515T-07 T (5KACC 16529 T 5NBRC 108923 T ).

Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere

“…Rihs et al (1993) reported that the DNA G+C content of the genus Roseomonas ranges from 65 to 71 mol%. However, Kim et al (2013) showed the DNA G+C content of R. aerophila was 73.0 mol%, slightly above values previously reported, and Dong et al (2014) reported a 63.0 mol% DNA G+C content for R. alkaliterrae, a value that was slightly below values for species in the genus Roseomonas. From a literature review, the highest content for a IP: 54.202.233.140…”

“…On: Sat, 12 May 2018 03:08:17 major fatty acid of this genus was for C 18 : 1 v7c under the conditions studied (Gallego et al, 2006;Yoon et al, 2007;Furuhata et al, 2008Furuhata et al, , 2013Yoo et al, 2008;Zhang et al, 2008;Venkata Ramana et al, 2010;Lopes et al, 2011;Kim et al, 2013;Nutaratat et al, 2013;Tonouchi & Tazawa, 2014;Chen et al, 2014;Dong et al, 2014;He et al, 2014, Kim & Ka, 2014 except Roseomonas lacus TH-G33 T , which had iso-C 16 : 0 as the predominant fatty acid (Jiang et al, 2006). Cells contained ubiquinone 10 (Q-10) as the major quinone (Kim et al, 2013;Dong et al, 2014;Chen et al, 2014).…”

“…Based on 16S rRNA gene sequence analysis, this genus belongs to the Alphaproteobacteria (Weyant & Whitney, 2005). At the time of writing, 23 species and two subspecies with validly published names are members of the genus Roseomonas, recently recognized species including Roseomonas aerophila (Kim et al, 2013) There have been reports on the isolation of Roseomonas species from various sources such as medical and environmental samples including plant phyllosphere. Together, these findings indicate that members of the genus Roseomonas exist widely in nature.…”

“…Cells contained ubiquinone 10 (Q-10) as the major quinone (Kim et al, 2013;Dong et al, 2014;Chen et al, 2014). With regard to the polyamine pattern, members of the genus Roseomonas have spermidine as the predominant compound (Sánchez-Porro et al, 2009).…”

“…Based on 16S rRNA gene sequence analysis, this genus belongs to the Alphaproteobacteria (Weyant & Whitney, 2005). At the time of writing, 23 species and two subspecies with validly published names are members of the genus Roseomonas, recently recognized species including Roseomonas aerophila (Kim et al, 2013), 'Roseomonas musae' (Nutaratat et al, 2013), Roseomonas tokyonensis (Furuhata et al, 2013), 'Roseomonas aceris' (Tonouchi & Tazawa, 2014), Roseomonas alkaliterrae (Dong et al, 2014), Roseomonas rhizosphaerae (Chen et al, 2014), 'Roseomonas sediminicola' (He et al, 2014) and Roseomonas soli (Kim & Ka, 2014). There have been reports on the isolation of Roseomonas species from various sources such as medical and environmental samples including plant phyllosphere.…”