Roseivivax isoporae sp. nov., isolated from a reef-building coral, and emended description of the genus Roseivivax

Abstract

A bacterial strain, isolated from a sample of reef-building coral (Isopora palifera) collected off the coast of southern Taiwan, was characterized using a polyphasic taxonomic approach. The strain, designated sw-2T, was Gram-staining-negative, aerobic, rod-shaped and motile, with subpolar flagella, and formed greyish pink colonies. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain sw-2T was most closely related to Roseivivax halodurans Och 239T (97.4 % sequence similarity) and Roseivivax halotolerans Och 210T (96.4 %). The novel strain did not require NaCl for growth and exhibited optimal growth at 35–40 °C, at pH 7.5–8.0 and with 3–7 % (w/v) NaCl. It produced bacteriochlorophyll a under aerobic conditions. Summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 63.7 %) predominated in the cellular fatty acid profile. The novel strain’s major respiratory quinone was ubiquinone Q-10 and its genomic DNA G+C content was 68.8 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sulfo-quinovosyl diacylglycerol and three uncharacterized phospholipids. The level of DNA–DNA relatedness between strain sw-2T and Roseivivax halodurans Och 239T was only 15.0 %. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of the novel strain from all established species of the genus Roseivivax . Based on the genotypic, phenotypic and chemotaxonomic data, strain sw-2T represents a novel species in the genus Roseivivax , for which the name Roseivivax isoporae sp. nov. is proposed. The type strain is sw-2T ( = LMG 25204T = BCRC 17966T).

Roseivivax roseus sp. nov., an alphaproteobacterium isolated from a solar saltern soil sample

“…At the time of writing, there are six species with validly published names, which were all isolated from saline environments (Suzuki et al, 1999;Park et al, 2010;Chen et al, 2012;Xiao et al, 2012;Wu et al, 2013). Here, we describe another Roseivivax strain, BH87090…”

Microbial communities in the nepheloid layers and hypoxic zones of the Canary Current upwelling system

“…c Data from (Gosink, Herwig, & Staley, 1997;Labrenz et al, 1999;Martens et al, 2006). d Data from (Chen, Sheu, Chen, Wang, & Chen, 2012;Zhang et al, 2013). e Data from (Cho & Giovannoni, 2006;Lin et al, 2014).…”

Roseivivax marinus sp. nov., isolated from deep water

“…All data are from this study except for the DNA G+C contents of the reference strains R. isoporae (Chen et al, 2012) and R. halodurans (Suzuki et al, 1999). +, Positive; 2, negative.…”

“…However, this description was emended by Park et al (2010) to motile or non-motile, positive or negative for BChl a, growing or not growing under anaerobic conditions, with phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid and unidentified lipids as common major polar lipids, and with genomic DNA G+C contents between 59.7 and 68.2 mol%. The genus was again emended by Chen et al (2012) to include that genomic DNA G+C contents range between 59.7 and 68.8 mol%, and that phosphatidylcholine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sulfo-quinovosyl diacylglycerol and another unidentified phospholipid are also detected. In this study, we report the isolation and description of a novel strain, ZL136 T , belonging to the genus Roseivivax based on a polyphasic taxonomic approach.…”