Roseivivax roseus sp. nov., an alphaproteobacterium isolated from a solar saltern soil sample

Abstract

A pink, Gram-stain-negative, motile, halotolerant bacterium with subpolar flagellum, designated strain BH87090T, was isolated from a saline soil sample collected from the south-west coastal area of South Korea (125° 58′ 58.08″ E 34° 45′ 37.32″ N). The isolate formed opaque pink to red colonies on marine agar plates at 30 °C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylcholine and one unidentified phospholipid. The sole respiratory quinone was ubiquinone-10 (Q-10). The major cellular fatty acids were C18 : 1ω7c, C19 : 0 cyclo ω8c, C16 : 0 and 11-methyl C18 : 1ω7c. The genomic DNA G+C content was 61.8 mol%. These chemotaxonomic characteristics were all consistent with specific properties of the genus Roseivivax . Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate affiliated to the cluster with members of the genus Roseivivax in the Roseobacter clade, which suggested that the strain belonged to the genus Roseivivax . However, the low 16S rRNA gene similarities (93.5–95.3 %) of strain BH87090T with all the members of the genus Roseivivax indicated that it represented a novel species of the genus Roseivivax . On the basis of phenotypic and genotypic data, strain BH87090T should be classified as a novel species of the genus Roseivivax . The name Roseivivax roseus sp. nov. is proposed, with strain BH87090T ( = DSM 23042T = KCTC 22650T) as the type strain.

Análisis comparativo de la expresión de proteinas de Tislia consotesis en respuesta a cambios de la salinidad externa

“…Igualmente, la clase α-Proteobacteria contiene tanto halotolerantes como halófilos extremos y moderados. Los microorganismos halotolerantes aislados de ambientes marinos, minas salinas, suelos y lagos salinos, han sido reportados en los géneros Pelagibacterium (Xu et al 2011), Hoeflea (Jung et al 2013), Salinarimonas (Liu et al 2010a), Martelella (Rivas et al 2005), Roseivivax (Zhang et al 2014), Methyloceanibacter (Takeuchi et al 2014), Thalasoccus (Lee et al 2007) y Sneathiella (Jordan et al 2007).…”

“…El análisis shotgun proteómics proporciona una medida indirecta de las proteínas a través de los péptidos derivados de la digestión proteolítica de las proteínas intactas. La identificación de péptidos se realiza a partir de la comparación de los espectros de masa derivados de la fragmentación de los péptidos con los espectros de masa generados a partir de digestión in silico de una base de datos de proteínas (Zhang et al 2013).…”