ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation

Abstract

Main conclusion Reactive oxygen species (ROS)-induced oxidative stress results in low success or even total failure of cryopreservation. Better understanding of how the plant establishes resistance/tolerance to ROS-induced oxidative stress facilitates developments of robust cryopreservation procedures. Abstract Cryopreservation provides a safe and efficient strategy for long-term preservation of plant genetic resources. ROS-induced oxidative stress caused damage to cells and reduced the ability of the plant to survive following cryopreservation, eventually resulting in low success or even total failure. This paper provides updated and comprehensive information obtained in the past decade, including the following: (1) ROS generations and adaptive responses of antioxidant systems during cryopreservation; (2) expressions of oxidative stress-associated genes and proteins during cryopreservation; (3) ROS-triggered programmed cell death (PCD) during cryopreservation; and (4) exogenous applications of enzymatic and non-enzymatic antioxidants in improving success of cryopreservation. Prospects for further studies are proposed. The goal of the present study was to facilitate better understanding of the mechanisms by which the plant establishes resistance/tolerance to oxidative stress during cryopreservation and promote further studies toward the developments of robust cryopreservation procedures and wider application of plant cryobiotechnology.

Effect of Cryopreservation Method Supported with Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia discolor

“…Abiotic stressors during cryopreservation (explant excision, osmotic injury, tissue dehydration, and temperature changes) promote an overproduction of reactive oxygen species (ROS) that causes oxidative damage [ 29 , 62 ]. Moderate ROS production is necessary for secondary signalling molecules [ 63 ].…”

“…Scavenging these surplus radicals may achieve tolerance to such harmful effects [ 65 ]. Published reports have employed antioxidants such as ascorbic acid, glutathione, and melatonin to directly remove the ROS and protect cellular functions, thus improving the chances of survival [ 35 , 36 , 62 , 66 , 67 , 68 ]. Studies describing the protective role of melatonin and enhancement in the recovery of cryopreserved shoot tips of American elm and yam were documented [ 66 , 69 ].…”

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

“…As cryopreservation procedures also resulted in an outburst of reactive oxygen species in plant tissues (Chen et al 2015;Ren et al 2021), one of the reasons for lowered tolerance in cells of AD might be the lack of functional chloroplasts, which regulated the plant responses to abiotic and biotic stress conditions (Mamaeva et al 2020). Nevertheless, in addition to the free water content which is distinguishable by the vacuole size that affected the 'live or die' of cells after cryopreservation, the ability of cells in response to osmotic stress should also be considered and investigated for better insight of shoot tip recovery after cryopreservation.…”

Shoot tip cryotherapy for plant pathogen eradication

“…The shoot tip excision, preculture, osmoprotection and cryoprotection, dehydration, freeze–thaw cycle and unloading treatments in cryotherapy procedures can all induce reactive oxygen species generation (Ren et al, 2021). Reactive oxygen species‐induced oxidative stresses have been documented to induce epigenetic and somatic variations (Wang, Bi, et al, 2021).…”