ROS generation and oxidative stress induced by hexachlorobenzene in human intestinal caco-2 cells

Chalouati, Hela; Boussoufa, Dhouha; Payrastre, Laurence

doi:10.1016/j.toxlet.2018.06.876

Cited by 4 publications

(9 citation statements)

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“…one order of magnitude lower than our lowest concentration, with a dose-dependent increase in the observed effect up to the maximum tested concentration of 570 mg L À1 , but this effect was only observed aer 14 days of exposure. 27 Membrane integrity in our study appeared to be unaffected by HCB, with the exception of a transient increase in CFDA-AM uorescence in cells exposed in suspension (Fig. 2b).…”

Section: Characterisation Of the Passive Dosing Setup For Hcbmentioning

confidence: 42%

“…The ndings vary for different cell types and concentration ranges, with some studies having dosed HCB at concentrations several orders of magnitude above its maximum water solubility. 27,28 Resulting concentration instability may be one of the reasons for divergence in reported concentrationresponses as outlined below.…”

Section: Characterisation Of the Passive Dosing Setup For Hcbmentioning

confidence: 99%

“…Finally, a signicant increase in DNA breaks was also found in human colon carcinoma cells exposed to 0.1 and 114 mg L À1 HCB for 14 days. 27 It is difficult to compare the results of different genotoxicity studies not only due to different species and endpoints assessed, but also due to the different exposure setups and vast differences in HCB concentrations applied. Nonetheless, there is previous evidence in the literature that HCB has the potential to cause DNA damage.…”

Section: Characterisation Of the Passive Dosing Setup For Hcbmentioning

confidence: 99%

“…Most effect evaluation studies to date have been conducted by dissolving HCB in an organic solvent. [27][28][29][30] However, stable exposure concentrations cannot be reached in this way. [31][32][33] It was, for example, shown that concentrations of a PAH and a chlorobenzene decrease dramatically over time in a solvent-spiked setup: concentrations of benzo(a)pyrene and 1,2,4-trichlorobenzene were reduced to 64% and 11% of the original concentrations, respectively, 48 hours aer direct spiking.…”

Section: Introductionmentioning

confidence: 99%

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Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions

et al. 2019

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Section: Characterisation Of the Passive Dosing Setup For Hcbmentioning

confidence: 42%

Section: Characterisation Of the Passive Dosing Setup For Hcbmentioning

confidence: 99%

Section: Characterisation Of the Passive Dosing Setup For Hcbmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions

et al. 2019

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“…In another study, the researcher observed that HCB imposed dose‐dependent cytotoxicity on colon cancer Caco‐2 cells. HCB led to DNA damage and the destruction of cell integrity, promoted the production of H 2 O 2 and increased the activities of numerous antioxidant enzymes, including SOD, CAT and glutathione peroxidase (GPx) (Chalouati et al., 2015). In Cyprinus Carpio , HCB affected the antioxidant status of the liver and brain.…”

Section: Discussionmentioning

confidence: 99%

The endocrine disruptor hexachlorobenzene can cause oxidative damage in the testis of mice

Dou

Mou

et al. 2021

Andrologia

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Hexachlorobenzene (HCB) was originally used as a common pesticide but was subsequently identified as an endocrine-disrupting chemical (Chiappini et al., 2019). Consequently, the use of HCB as a pesticide was terminated in the mid-1970s and is only used in laboratory scenarios at present (Iatrou et al., 2019;Yu et al., 2019). However, since HCB degrades slowly in natural environments and can accumulate in organisms, HCB residues have been detected in soil, water, humans, animals and plants in many regions and countries across the world. As a consequence, HCB remains one of the largest environmental pol-

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Valorization of Onion Waste by Obtaining Extracts Rich in Phenolic Compounds and Feasibility of Its Therapeutic Use on Colon Cancer

et al. 2022

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In this study, the total phenolic content, the antioxidant and antiproliferative activities of onion waste extracts were characterized. Some phenolic compounds present in the extracts were also identified and quantified by HPLC-DAD. Additionally, an in-silico analysis was performed to identify the phenolic compounds with the highest intestinal absorption and Caco-2 permeability. The onion extract possessed a high amount of phenolic compounds (177 ± 9 mg/g extract) and had an effective antioxidant capacity measured by ABTS, FRAP and DPPH assays. Regarding the antiproliferative activity, the onion extracts produced cell cycle arrest in the S phase with p53 activation, intrinsic apoptosis (mitochondrial membrane potential modification) and caspase 3 activation. Likewise, onion waste increased intracellular ROS with possible NF-kB activation causing a proteasome down regulation. In addition, the extracts protected the intestine against oxidative stress induced by H2O2. According to the in-silico analysis, these results could be related to the higher Caco-2 permeability to protocatechuic acid. Therefore, this study provides new insights regarding the potential use of these types of extract as functional ingredients with antioxidant and antiproliferative properties and as medicinal agents in diseases related to oxidative stress, such as cancer. In addition, its valorization would contribute to the circular economy.

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ROS generation and oxidative stress induced by hexachlorobenzene in human intestinal caco-2 cells

Cited by 4 publications

References 0 publications

Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions

Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions

The endocrine disruptor hexachlorobenzene can cause oxidative damage in the testis of mice

Valorization of Onion Waste by Obtaining Extracts Rich in Phenolic Compounds and Feasibility of Its Therapeutic Use on Colon Cancer

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