Roots of the wetland plants Typha latifolia and Phragmites australis are inhabited by methanotrophic bacteria in biofilms

Faußer, Anna; Hoppert, Michael; Walther, Paul; Kazda, Marian

doi:10.1016/j.flora.2012.09.002

Cited by 35 publications

(25 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our metagenomic evidence that methanotrophs occur in the microbiotas of aquatic streptophyte algae in addition to those of nonstreptophyte periphytic freshwater algae (Zulkifly et al 2012;Graham et al 2014a) is important because freshwaters are known hot spots of methane production (Bastviken et al 2011). Widespread algal-methanotroph associations should be expected to occur because, like peat mosses and other wetland plants (Fausser et al 2012), photosynthetic algae provide a reliable supply of oxygen needed for methanotrophy and an extensive area of stable attachment surface in methane-rich habitats. The results we report here and elsewhere (Zulkifly et al 2012;Graham et al 2014a) suggest that, as in the case of peat moss-methanotroph associations, algal-associated methanotrophy may help to control the accumulation of methane in Earth's atmosphere today and also has done so in the remote past.…”

Section: Biogeochemical Significance Of Vitamin B 12 Biosynthesis Andmentioning

confidence: 99%

Microbiomes of Streptophyte Algae and Bryophytes Suggest That a Functional Suite of Microbiota Fostered Plant Colonization of Land

Knack¹,

Wilcox²,

Delaux³

et al. 2015

International Journal of Plant Sciences

100

View full text Add to dashboard Cite

Premise of research. The origin of land plants catalyzed key changes in Earth's atmosphere and biota. Microbial associations likely nurtured earliest plants and influenced their biogeochemical roles. Because angiosperm and animal microbiomes-bacteria, archaea, microbial eukaryotes, and genes that promote host survival-are known to display lineage effects, we hypothesized that microbiomes of early-diverging modern bryophytes and phylogenetically closely related green algae might likewise reveal commonalities reflecting ancestral traits.Methodology. New metagenomic sequence data were obtained for the late-diverging streptophyte algae Chaetosphaeridium globosum and Coleochaete pulvinata and the liverwort Conocephalum conicum, representing early-diverging land plants. New 16S rDNA amplicon sequences were acquired for the charalean Nitella tenuissima. Sequence data were used to infer bacterial genera and fungi for comparisons among streptophyte microbiota and with our published microbiome data for the outgroup chlorophyte Cladophora. To enhance evolutionary signal, taxa were sampled in the same time frame and from geographically close locales. Streptophyte metagenomic data were also probed for protein markers of significant physiological and biogeochemical functions: NifH indicating nitrogen fixation, particulate MMo indicating methane oxidation, and vitamin B 12 (cobalamin) indicating biosynthetic pathway enzymes.Pivotal results. Microbiota of studied streptophytes consistently included diverse N-fixing cyanobacteria and/or Rhizobiales, as well as methanotrophs and early-diverging fungi, and were more similar to each other than to Cladophora microbiota. Streptophyte metagenomic data indicated diverse nifH (nitrogen fixation) and pMMo (methane oxidation) marker sequences and vitamin B 12 pathway genes. Glomalean fungi occurred with Conocephalum, consistent with field studies of modern liverworts and microfossil evidence for cooccurrence of glomaleans and early land plants. Conclusions.A suite of N fixers, methanotrophs, cobalamin producers, and early-diverging fungi was consistently associated with modern streptophyte algae and bryophytes studied, suggesting features of early land plants that have played significant, previously unrecognized roles in global nitrogen and carbon cycling for hundreds of millions of years.

show abstract

Section: Biogeochemical Significance Of Vitamin B 12 Biosynthesis Andmentioning

confidence: 99%

Microbiomes of Streptophyte Algae and Bryophytes Suggest That a Functional Suite of Microbiota Fostered Plant Colonization of Land

Knack¹,

Wilcox²,

Delaux³

et al. 2015

International Journal of Plant Sciences

100

View full text Add to dashboard Cite

show abstract

“…Through a 16S rRNA gene Illumina MiSeq sequencing, Pietrangelo et al (2018) reported the bacterial community structure on the root surface of P. australis was indeed different from that of T. latifolia. In addition, Fausser et al (2012) have suggested that methylotrophic bacteria live in the root zones of P. australis and T. latifolia. However, the community structure of rootassociated methanotrophs and the relationship between the root-associated methanotrophs (functional bacteria) and plant species remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

et al. 2020

View full text Add to dashboard Cite

Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmoA gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmoA based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter. In T. angustifolia, these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.

show abstract

“…The root zone (root and rhizosphere sediment) of wetland plants is under aerobic conditions (Armstrong et al, 2000) and allows the growth of aerobic methanotrophs (Type I and Type II) that utilize methane and methanol as their sole carbon and energy sources (Hanson and Hanson, 1996). Previous studies suggested that methylotrophic bacteria and other bacterial groups live in the root zones of Phragmites australis and Typha latifolia (Chen et al, 2012;Fausser et al, 2012); however, it currently remains unclear whether differences exist in the community structure of root-associated methanotrophs among plant species, and how these methanotrophs are distributed in the root tissues of macrophytes has not yet been clarified.…”

mentioning

confidence: 99%

Enrichment of Type I Methanotrophs with <i>nirS</i> Genes of Three Emergent Macrophytes in a Eutrophic Wetland in China

et al. 2020

View full text Add to dashboard Cite

The pmoA gene, encoding particulate methane monooxygenase in methanotrophs, and nirS and nirK genes, encoding bacterial nitrite reductases, were examined in the root and rhizosphere sediment of three common emergent macrophytes (Phragmites australis, Typha angustifolia, and Scirpus triqueter) and unvegetated sediment from eutrophic Wuliangsuhai Lake in China. Sequencing analyses indicated that 334 out of 351 cloned pmoA sequences were phylogenetically the most closely related to type I methanotrophs (Gammaproteobacteria), and Methylomonas denitrificans-like organisms accounted for 44.4% of the total community. In addition, 244 out of 250 cloned nirS gene sequences belonged to type I methanotrophs, and 31.2% of nirS genes were the most closely related to paddy rice soil clone SP-2-12 in Methylomonas of the total community. Three genera of type I methanotrophs, Methylomonas, Methylobacter, and Methylovulum, were common in both pmoA and nirS clone libraries in each sample. A quantitative PCR (qPCR) analysis demonstrated that the copy numbers of the nirS and nirK genes were significantly higher in rhizosphere sediments than in unvegetated sediments in P. australis and T. angustifolia plants. In the same sample, the nirS gene copy number was significantly higher than that of nirK. Furthermore, type I methanotrophs were localized in the root tissues according to catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Thus, nirS-carrying type I methanotrophs were enriched in macrophyte root and rhizosphere sediment and are expected to play important roles in carbon/nitrogen cycles in a eutrophic wetland.

show abstract

Roots of the wetland plants Typha latifolia and Phragmites australis are inhabited by methanotrophic bacteria in biofilms

Cited by 35 publications

References 54 publications

Microbiomes of Streptophyte Algae and Bryophytes Suggest That a Functional Suite of Microbiota Fostered Plant Colonization of Land

Microbiomes of Streptophyte Algae and Bryophytes Suggest That a Functional Suite of Microbiota Fostered Plant Colonization of Land

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Enrichment of Type I Methanotrophs with <i>nirS</i> Genes of Three Emergent Macrophytes in a Eutrophic Wetland in China

Contact Info

Product

Resources

About