Root inoculation of strawberry with the entomopathogenic fungi Metarhizium robertsii and Beauveria bassiana reduces incidence of the twospotted spider mite and selected insect pests and plant diseases in the field

Canassa, Fernanda; Esteca, Fernanda de Cássia Neves; Moral, Rafael de Andrade; Meyling, Nicolai V.; Klingen, Ingeborg; Delalibera, Ítalo

doi:10.1007/s10340-019-01147-z

Cited by 42 publications

(38 citation statements)

References 79 publications

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“…The present results demonstrate the persistence of isolates of Metarhizium spp., B. bassiana and C. fumosorosea 6 months post inoculations under greenhouse conditions. In a field study in Brazil, Canassa et al . reported the recovery of M. robertsii ESALQ 1622 and B. bassiana ESALQ 3375 from strawberry leaves and soil adjacent to the roots 180 days after root inoculation.…”

Section: Discussionmentioning

confidence: 99%

“…73 The present results demonstrate the persistence of isolates of Metarhizium spp., B. bassiana and C. fumosorosea 6 months post inoculations under greenhouse conditions. In a field study in Brazil, Canassa et al 74 reported the recovery of M. robertsii ESALQ 1622 and B. bassiana ESALQ 3375 from strawberry leaves and soil adjacent to the roots 180 days after root inoculation. In addition, reduced numbers of T. urticae adults were observed on strawberry plants inoculated with these isolates of M. robertsii and B. bassiana compared to non-inoculated control plants under commercial cultivation regimes, indicating the promising field application perspectives of this biocontrol strategy.…”

Section: Discussionmentioning

confidence: 99%

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Fungal isolate and crop cultivar influence the beneficial effects of root inoculation with entomopathogenic fungi in strawberry

Canassa

D’Alessandro

Sousa

et al. 2019

Pest Management Science

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BACKGROUND Root inoculations of crop plants with beneficial fungi constitute a promising strategy for growth promotion and control of above‐ground pests and diseases. Here, strawberry roots (cultivar ‘Albion’ and ‘Pircinque’) were inoculated with 25 different Brazilian entomopathogenic fungal isolates of three genera and the effects on Tetranychus urticae oviposition and plant growth were evaluated in greenhouse experiments. RESULTS Reductions in the number of T. urticae eggs compared to control treatments were observed on both cultivars inoculated with almost all isolates. For the cultivar ‘Albion’, Metarhizium anisopliae (ESALQ 1604, ESALQ 1669), M. robertsii (ESALQ 1622, ESALQ 1635), Metarhizium sp. Indet. (ESALQ 1684) and Beauveria bassiana (ESALQ 3323) increased dry weight of roots and leaves, and fruit yield, while M. robertsii (ESALQ 1634), Metarhizium sp. Indet. (ESALQ 1637) and (ESALQ 1636) enhanced fruit yield and dry weight of leaves, respectively. For the cultivar ‘Pircinque’, M. anisopliae (ESALQ 1669) was the only isolate observed to increase dry weight of roots. CONCLUSION The results suggest that inoculation of strawberry roots with entomopathogenic fungi may be an innovative strategy for pest management above ground. Furthermore, these inoculations may also stimulate plant growth and strawberry production, but the effects depend on fungal strains and crop cultivar. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fungal isolate and crop cultivar influence the beneficial effects of root inoculation with entomopathogenic fungi in strawberry

Canassa

D’Alessandro

Sousa

et al. 2019

Pest Management Science

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“…There is now a plethora of evidence that some endophytic entomopathogenic fungi, particularly Beauveria bassiana and Lecanicillium lecanii (formerly Verticillium lecanii) may also exhibit antagonistic activity against plant pathogens besides their well-known biological control potential (Ownley et al 2004;Ownley et al 2008;Vega et al 2009 Canassa et al 2020). For example, B. bassiana strain 11-98, when applied as a seed treatment, was found to suppress damping-off disease caused by Rhizoctonia solani and Pythium myriotylum in tomato (Ownley et al 2004) and cotton seedlings (Ownley et al 2008).…”

Section: Endophytic Insect Pathogenic Fungi As Plant Disease Antagonistsmentioning

confidence: 99%

Entomopathogenic Fungi as Endophyte: An Emerging Tool for Biological Pest Control

Dash¹,

Bamisile²,

Nondi³

et al. 2020

JBAH

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Biological control using fungal entomopathogen is an effective and sustainable alternative to chemical control of insect pests. However, despite enormous potential, they have not been fully utilized due to their susceptibility to unfavorable abiotic factors such as ultraviolet (UV) light and low humidity. An increasing number of studies in the last decade demonstrates that entomopathogenic fungi have wider ecology than previously thought. They have been found to colonize various plant naturally as endophyte as well as they have been successfully established in plants with various inoculation methods. In planta presence of these fungi can overcome the impediments arise from inundative application of fungal suspension as they have been found not only protect the host plant from pest and disease but also act as a plant growth promoter. In this review, we discussed the multiple benefits provided by fungal entomopathogens following their association with the host plant as an endophyte and highlights the prospects of incorporating fungal endophytes as an integral part of pest management programs towards sustainable crop production.

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“…This genus has a worldwide distribution and the different species posess a versatile lifestyle allowing them to 1) inhabit the soil Kepler et al, 2015;Rezende et al, 2015;Iwanicki et al, 2019, 2) infect insects and mites (Vega et al, 2009;Zimmermann, 2007). 3) live in association with roots or as plant endophytes (Wyrebek et al, 2011;Sasan and Bidochka, 2013;Liao et al, 2014;Steinwender et al, 2015;Canassa et al, 2019aCanassa et al, , 2019b); 4) and act as antagonist of plant pathogens .…”

Section: The Genus Metarhiziummentioning

confidence: 99%

“…are known mainly as biological control agents against insects, their entomopatogenicity characteristic arises later in the evolutionary history of Metarhizium in relation to its life-style associated with plants (Gao et al, 2011). Nonetheless, many investigations have focussed on the ability of these fungi to act as biostimulants of commercial plants (Sansan and Bidochka, .2013Golo et al, 2014;Jaber and Enkerli, 2017;Canassa et al, 2019aCanassa et al, , 2019b in addition to its effects in controlling insect and mites.…”

Section: Rileyi (Formely Nomureae Rileyi)mentioning

confidence: 99%

Advances in >i<Metarhizium>/i< blastospores production and formulation and transcriptome studies of the yeast and filamentous growth

Iwanicki¹

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Advances in Metarhizium blastospores production and formulation and transcriptome studies of the yeast and filamentous growth Biological control of pests is a growing market in the world. It is expected that the use entomopathogenic fungi to control pests will take an important share of this market. Most fungal products in the world are based on aerial conidia produced by solid fermentation using cereal grains. An alternative for aerial conidia is the use of blastospores, yeast-like hydrophilic cells that can be produced in large amounts by liquid fermentation in a short time (<4 days), in a small space and with low hand labor compared to the solid fermentation method. Therefore, the main objectives of the present studies were first to optimize a liquid culture medium for low cost production of Metarhizium blastopores; second: to assess the bioactivity of air-dried blastospores against the cattle-tick Rhipicephalus microplus; third: to develop an airdried and spray-dried Metarhizium blastospore formulation with bioactivity against the corn-leafhopper Dalbulus maidis; fourth: to improve the shelf-life of the best air-dried and spray-dried formulations stored in refrigerated (± 4°C) and in ambient conditions (28°C) using oxygen and moistures absorbrs or vacuum and fifth: to use comparative genome-wide transcriptome analyses to determine changes in gene expression between the filamentous and blastospore growth phases in vitro to characterize physiological changes and metabolic signatures associated with M. anisopliae and M. rileyi dimorphism. We showed that blastospore production of Metarhizium is isolate-and species-dependent. Glucose-enriched cultures inoculated with pre-cultures improved yields reaching optimal growth for Metarhizium robertsii ESALQ1426 (5.9 × 10 8 blastospores/mL) within 2 d. Resultant air-dried blastospores of ESALQ1426 were proved to quickly kill R. micropulus larvae with an efficiency comparable to that of conidia. We argue that both osmotic pressure, induced by high glucose titers, and isolate selection are critical to produce high yields of blastospores that hold promise to control R. microplus larvae. Fermentation experiments based on growth kinetics defined a low-cost medium using 80 g/L corn steep liquor as the most suitable nitrogen source for inducing blastospore growth in M. robertsii (4.7 × 10 8 cells/mL) in only 2 days of cultivation at a total cost of $0.30 USD per L. The dried blastospores were as high virulence as fresh cells to D. maidis adults fed on maize plants. Co-formulants were selected to compose formulations that allowed to keep M. roberstii blastospore viability after drying. The most promising resulting spray-dried and air-dried were as infective as fresh blastopores to the corn leafhopper inducing mortality rates ranging from 60.3 to 78.2% after spraying with 5 × 10 7 blastospores/mL. The LC 50 was significantly higher for spray-dried formulation (2.42 × 10 7) than for the air-dried formulation (4.65 × 10 6), suggesting a possible detrimental effect of the former tech...

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Root inoculation of strawberry with the entomopathogenic fungi Metarhizium robertsii and Beauveria bassiana reduces incidence of the twospotted spider mite and selected insect pests and plant diseases in the field

Cited by 42 publications

References 79 publications

Fungal isolate and crop cultivar influence the beneficial effects of root inoculation with entomopathogenic fungi in strawberry

Fungal isolate and crop cultivar influence the beneficial effects of root inoculation with entomopathogenic fungi in strawberry

Entomopathogenic Fungi as Endophyte: An Emerging Tool for Biological Pest Control

Advances in >i<Metarhizium>/i< blastospores production and formulation and transcriptome studies of the yeast and filamentous growth

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