Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms Between Resistant and Susceptible Tobacco Cultivars Against Black Shank Disease

Zhang, Chengsheng; Feng, Chao; Zheng, Yanfen; Wang, Jing; Wang, Fenglong

doi:10.3389/fpls.2020.559775

Cited by 33 publications

(32 citation statements)

References 64 publications

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“…Effect of VB1 at different concentrations on mycelial growth and sporangium production of P. nicotianaein vitro VB1 stock solution was prepared in sterile distilled water, and the solution was ltered through a micro ltration membrane. The effect of VB1 on mycelial growth of P.nicotianae was evaluated on PDA plates according to the method of Zhang et al 18,19 . A 7-mm P. nicotianae agar disk from actively growing 15-day mycelium of the pathogen was transferred into a new PDA plate with different concentrations of VB1 (0, 1, 2, 5, 10, 20 and 50 mM).…”

Section: Plant Materials and Inoculum Preparationmentioning

confidence: 99%

Priming Effect of Thiamine on the Enhancement of Induced Resistance to the Plant Disease Phytophthora Nicotianae in Tobacco

Liu

Suohui

Chen

et al. 2021

Preprint

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Induced resistance by elicitors is considered to be an eco-friendly strategy to stimulate plant defense against pathogen attack. Thiamine (vitamin B1,VB1) can act as a plant defence trigger, or priming agent, leading to a rapid counterattack on pathogen invasion.To date, the mechanisms by which VB1 provides protection against plant disease have yet to be fully elucidated, expecially no reports about VB1 treatment influenced the development of Phytophthora nicotianae in plant. Tobacco black shank (TBS) caused by P. nicotianae is destructive to almost all tobacco cultivars and is widespread in many tobacco-growing countries. In the present study, the priming effect of VB1 on tobacco against the disease P. nicotianae and its biochemical and molecular impact on plant defense mechanisms were evaluated. Base on the effect of VB1 on mycelial growth and zoospore formation, the appropriate VB1 treatment was used in protecting tobacco against P. nicotianae. For VB1 pretreatment, tobacco exhibited a significant reduction in disease severity. Consistent with the occurrence of induced resistance, the pronounced increase in H2O2 level, phenylalanine ammonia lyase (PAL) and peroxidase (POD) activities were observed. For defense reactions, VB1 promoted the increases of H2O2, SA and lignin contents. Moreover, the expressions of PR1, PR5, NPR1, PAL, CM1, H1N1 and EFE26 were induced by VB1, which also involved in defense reactions. Our findings indicate that the priming effect of VB1 may partially depend on the production of the callose deposition, H2O2 accumulation, and hormone SA production.

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Section: Plant Materials and Inoculum Preparationmentioning

confidence: 99%

Priming Effect of Thiamine on the Enhancement of Induced Resistance to the Plant Disease Phytophthora Nicotianae in Tobacco

Liu

Suohui

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…P. nicotianae race 0 strain JM01 with high pathogenicity was obtained from the Integrated Pest Management Key Laboratory of China Tobacco, Qingdao, China [22]. P. nicotianae cultured on millet were used to field inoculation.…”

Section: Preparation Of Inoculum Of Phytophthora Nicotianaementioning

confidence: 99%

“…The zoospores of P. nicotianae were produced according to the method described in Zhang et al [22]. Briefly, P. nicotianae was cultured for 21 days on an oat medium (OA) plate.…”

Section: Preparation Of Inoculum Of Phytophthora Nicotianaementioning

confidence: 99%

Section: Effects Of Root Exudates On Phytophthora Nicotianaementioning

confidence: 99%

“…The effects of root exudates on P. nicotianae were evaluated by testing the effects of the exudates on the mycelium growth rate and spore germination [22]. P. nicotianae strain JM01 tested was preserved in our laboratory [22]. Root exudates were diluted to the final content 20 mg ml −1 , filtered with a 0.22 µm millipore filtration membrane, and blended to sterilized OA (cooled to 45 • C) with the volume ratio of 1:1, followed by pouring into Petri dishes.…”

Section: Effects Of Root Exudates On Phytophthora Nicotianaementioning

confidence: 99%

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Rootstock-Scion Interaction Affects the Composition and Pathogen Inhibitory Activity of Tobacco (Nicotiana tabacum L.) Root Exudates

Zhang

Zheng

Peng

et al. 2020

Plants

Self Cite

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The composition and allelopathy to Phytophthora nicotianae (the causal agent of tobacco black shank disease) of root exudates from a resistant tobacco (Nicotiana tabacum L.) cultivar Gexin 3, a susceptible cultivar Xiaohuangjin 1025 and their reciprocal grafts were investigated. Grafting with disease-resistant rootstock could improve resistance to black shank; this is closely related to the allelopathy of root exudates. The root exudates from the resistant cultivar inhibited the growth of P. nicotianae, while those from the susceptible cultivar promoted the growth; the grafting varieties had intermediate properties. The root exudate composition differed among cultivars. Gexin 3 was rich in esters and fatty acids, while Xiaohuangjin 1025 contained more hydrocarbons and phenolic acids. The composition of root exudates of grafted cultivars as well as their allelopathy to P. nicotianae were altered, and tended to be close to the composition of cultivar used as rootstock. Eugenol, 4-tert-butylphenol, mono (2-ethylhexyl) phthalate, 4-hydroxybenzoic acid, 2,6-di-tert-butylphenol, dipropyl phthalate, and methyl myristate were identified as the main compounds contributing to inhibitory properties of root exudates. Sorbitol was suggested to play a role in disease induction. Overall, rootstock–scion interaction affected the composition of tobacco root exudates, which may be attributed to the different disease resistance among grafted plants, rootstock and scion.

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Updating the Impact of Drought on Root Exudation: A Strigolactones Perspective

Singh

Chattopadhyay

Gupta

2023

J Plant Growth Regul

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Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms Between Resistant and Susceptible Tobacco Cultivars Against Black Shank Disease

Abstract: play a role in inducing plant defense responses, showed potential for disease control application. This study provides an insight into possible disease resistance mechanisms of root exudates, and attempts the beneficial utilization of these secondary metabolites of plants.

Cited by 33 publications

References 64 publications

Priming Effect of Thiamine on the Enhancement of Induced Resistance to the Plant Disease Phytophthora Nicotianae in Tobacco

Priming Effect of Thiamine on the Enhancement of Induced Resistance to the Plant Disease Phytophthora Nicotianae in Tobacco

Rootstock-Scion Interaction Affects the Composition and Pathogen Inhibitory Activity of Tobacco (Nicotiana tabacum L.) Root Exudates

Updating the Impact of Drought on Root Exudation: A Strigolactones Perspective

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