Updating the Impact of Drought on Root Exudation: A Strigolactones Perspective

Singh, N. P.; Chattopadhyay, Debasis; Gupta, Santosh Kumar

doi:10.1007/s00344-023-11061-5

Cited by 2 publications

(1 citation statement)

References 181 publications

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“…Flavonoids constitute a broad class of specialized polyphenols that share the C 6 -C 3 -C 6 basic structure and undergo a series of secondary modifications (e.g., glycosylation, methylation, acylation) that enable their classification into various classes like flavones, isoflavonoids, flavonols, and anthocyanins (Yonekura-Sakakibara et al, 2019). Flavonoids are among the most abundant compounds released by rhizodeposition (Chaparro et al, 2013;Wang et al, 2022): they represented 37% of total secondary metabolites released by Arabidopsis thaliana (Narasimhan et al, 2003) and reached a higher abundance compared with exuded primary metabolites in Quercus ilex upon water shortage stress (Singh et al, 2023). A metabolome investigation of the root exudate composition of Panax notoginseng, Zea mays, Nicotiana tabacum, and Perilla frutescens showed a high chemical diversity, with flavonoids being among the dominating compounds in terms of abundance and with differential patterns in terms of composition, contributing to the uniqueness of the exudation profile in the different plants (Shi et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Flavonoids influence key rhizocompetence traits for early root colonization and PCB degradation potential of Paraburkholderia xenovorans LB400

Ghitti,

Rolli,

Vergani

et al. 2024

Front. Plant Sci.

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IntroductionFlavonoids are among the main plant root exudation components, and, in addition to their role in symbiosis, they can broadly affect the functionality of plant-associated microbes: in polluted environments, for instance, flavonoids can induce the expression of the enzymatic degradative machinery to clean-up soils from xenobiotics like polychlorinated biphenyls (PCBs). However, their involvement in root community recruitment and assembly involving non-symbiotic beneficial interactions remains understudied and may be crucial to sustain the holobiont fitness under PCB stress.MethodsBy using a set of model pure flavonoid molecules and a natural blend of root exudates (REs) with altered flavonoid composition produced by Arabidopsis mutant lines affected in flavonoid biosynthesis and abundance (null mutant tt4, flavonoid aglycones hyperproducer tt8, and flavonoid conjugates hyperaccumulator ttg), we investigated flavonoid contribution in stimulating rhizocompetence traits and the catabolic potential of the model bacterial strain for PCB degradation Paraburkholderia xenovorans LB400.ResultsFlavonoids influenced the traits involved in bacterial recruitment in the rhizoplane by improving chemotaxis and motility responses, by increasing biofilm formation and by promoting the growth and activation of the PCB-degradative pathway of strain LB400, being thus potentially exploited as carbon sources, stimulating factors and chemoattractant molecules. Indeed, early rhizoplane colonization was favored in plantlets of the tt8 Arabidopsis mutant and reduced in the ttg line. Bacterial growth was promoted by the REs of mutant lines tt4 and tt8 under control conditions and reduced upon PCB-18 stress, showing no significant differences compared with the WT and ttg, indicating that unidentified plant metabolites could be involved. PCB stress presumably altered the Arabidopsis root exudation profile, although a sudden “cry-for-help” response to recruit strain LB400 was excluded and flavonoids appeared not to be the main determinants. In the in vitro plant–microbe interaction assays, plant growth promotion and PCB resistance promoted by strain LB400 seemed to act through flavonoid-independent mechanisms without altering bacterial colonization efficiency and root adhesion pattern.DiscussionsThis study further contributes to elucidate the vast array of functions provided by flavonoids in orchestrating the early events of PCB-degrading strain LB400 recruitment in the rhizosphere and to support the holobiont fitness by stimulating the catabolic machinery involved in xenobiotics decomposition and removal.

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