Root bark extract of Calliandra portoricensis (Jacq.) Benth. chemoprevents N-methyl-N-nitrosourea-induced mammary gland toxicity in rats

Adefisan, Adedoyin O; Owumi, Solomon E.; Adaramoye, Oluwatosin A.

doi:10.1016/j.jep.2018.12.027

Cited by 12 publications

(14 citation statements)

References 46 publications

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“…41,42 In the light of these findings, rat ovarian and uterine LPO may have resulted from increased production of highly reactive ROS, that reacts with polyunsaturated fatty acids present in phospholipid constituents of cell membranes, naturally present in uterine and ovarian tissues of experimental rats, that may consequently lead to organ dysfunction. 24,41 Increases in MDA level, as well as a decrease in antioxidative defense enzymes, further corroborate previous reports on NMU-induced mammary carcinogenesis. 24 The GST and GPx activities were also drastically decreased after (NMU + BaP) intoxication.…”

Section: Discussionsupporting

confidence: 90%

“…injections for this study were based on previously published treatments in the literature. 21–23 CP was given orally thrice weekly, as previously described from published work from our laboratory, 24 while vincristine (VIN) was administered intraperitoneally twice weekly. At the end of the 10th week, records of experimental rat's final body weights were taken, before blood collection via retro-orbital venous puncture into plastic sample tubes (without heparin coat).…”

Section: Methodsmentioning

confidence: 99%

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Calliandra portoricensisameliorates ovarian and uterine oxido-inflammatory responses inN-methyl-N-nitrosourea and benzo[a]pyrene-treated rats

Adefisan

Madu

Owumi

et al. 2020

Exp Biol Med (Maywood)

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Reproductive dysfunction stemming from chemical agents may lead to infertility. We examined the protective effects of Calliandra portoricensis ( CP) extract on benzo[a]pyrene (BaP) and N-methyl- N-nitrosourea (NMU)-induced ovarian and uterine toxicity in rat, treated as follows: control (group 1), NMU + BaP (group 2), groups 3 and 4 received (NMU + BaP), and CP (50 and 100 mg/kg), respectively. Group 5: CP (100 mg/kg) alone, group 6: (NMU + BaP) and vincristine (VIN: 0.5 mg/kg) and group 7: VIN alone. Rats were injected at age 7, 10, and 13 weeks with single doses of NMU and BaP for 10 consecutive weeks. NMU + BaP significantly ( P < 0.05) increased ovarian and uterine weight, and decreased bodyweight, while the organo-somatic index (OSI) of uterus and ovary increased 2.3 and 1.4 folds, respectively. CP co-treatment ameliorated the observed weight changes. Lipid peroxidation increased by 58% in the ovary, accompanied by decreases in ovarian and uterine GST, GPx, catalase activities, and total sulfhydryl level in NMU + BaP-treated rats. Uterine and ovarian myeloperoxidase activities, as well as nitric oxide levels also increased. CP co-treatment ameliorated the observed changes in antioxidant enzymes and inflammatory biomarkers. Furthermore, histopathology revealed fibrotic ovarian stroma, while uterine endometrium was infiltrated with inflamed cells. Immunohistochemistry showed weak expression of FSH, LH, p53, caspase-3, and Bax, whereas progesterone, iNOS, and Bcl-2 were strongly expressed in NMU + BaP-treated rats. CP treatment restored the architecture of these tissues. Conclusively, the root bark fraction of CP decreases oxido-inflammatory damage in ovarian and uterine tissues of NMU- and BaP-treated rats. Impact statement Infertility resulting from reproductive impairment is traumatic in families. Exposure to chemicals may play insidious roles not easily connected to infertility. We examined benzo[a]pyrene (BaP), and N-methyl nitrosourea (NMU)-induced ovarian and uterine toxicity and the role of Calliandra portoricensis in mitigating toxicity. In a bid to illuminate folk medical claims cloaked in mystery, unearthing lost knowledge, advance natural chemopreventive agents, and report new evidence lacking in the literature attributed to CP. Although CP is known to exhibit anticonvulsant, antidiarrheal, antipyretic, antirheumatic, and analgesic effects in humans, its possible roles for mitigating toxicity stemming from inadvertent chemical exposures are reported here. Our findings affirm and further show that CP abates toxic response incumbent on oxidative damage and inflammatory responses associated with NMU and BaP exposure. Development of phytochemical derived from CP may serve as a potential natural therapy against chemical toxicities in individuals inadvertently exposed, and promote human health and reproductive satiety.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Calliandra portoricensisameliorates ovarian and uterine oxido-inflammatory responses inN-methyl-N-nitrosourea and benzo[a]pyrene-treated rats

Adefisan

Madu

Owumi

et al. 2020

Exp Biol Med (Maywood)

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“…This informed guess is consistent with the traditional application of the plant in the management of breast cancer in Southern Nigeria [58]. toxicity to tumour cell lines of the prostate [59] and chemo-prevents mammary gland toxicity in rodents [60]. All these reports provide an evidenced-based traditional application of the root of C. portoricensis in the management of various ailments including those caused by microbial pathogens or cancerrelated disease burdens.…”

Section: Discussionsupporting

confidence: 69%

Evaluation of peptide-rich root extracts of Calliandria portoriscensis (Jacq.) Benth (Mimosaceae) for in vitro antimicrobial activity and brine shrimp lethality

Ogbole

Ndabai

Akinleye

et al. 2020

BMC Complement Med Ther

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Background: Several Host defence peptides (HDPs) are low molecular weight (< 50 amino acids residues) peptides detected in several ethnomedicinal plants and have particularly gained research interest in recent times. Due to their wide range of bioactivity, occurrence, abundance and ability to induce very little resistance, they hold promising potentials in drug development. This study investigated the presence of bioactive peptides in the roots of Calliandra portoricensis (CPr) (Mimosaceae) and evaluated its antimicrobial activity against gram-negative and gram-positive bacteria.Methods: The crude peptide extract was obtained and pre-purified on pre-loaded tube of RP-C 18 solid phase cartridges (strata giga tube C18-E; 5 g, 20 mL, Phenomenex, Germany). Peptide enriched fraction was chemically analysed for arginine-rich/aromatic amino acid-rich peptides using a modified G-250 analytical stain and ninhydrin on thin layer chromatography (TLC) for a preliminary screening. Furthermore, MALDI TOF/TOF peptidomics was used to detect the presence and masses of the peptides. Extracts from CPr were used to test the ability to inhibit microbial growth using p-INT (Para-iodonitrotetrazolium violet) dye, with 0.1% gentamycin as positive control. The concentration that inhibits the growth of microorganisms by 50% (IC 50 ) were determined. Toxicity of the two extracts was accessed using freshly hatched nauplii of Artemia salina. Data analysis were evaluated using Microsoft excel and GraphPad Prism5.Results: Low molecular weight (LMW) peptides were detected in CPr using TLC and MALDI-TOF MS. Generally, the extracts exhibited good inhibition (70-95%) against the gram-negative and gram-positive bacteria, except MRSA6 typed strain. Enhanced activity was observed in the pre-purified peptide fraction than in the methanol crude, except on MRSA6. The greatest antimicrobial inhibition by pre-purified peptide fraction was against MRSA22 (IC 50 = 0.69 ± 0.33 μg/mL). The crude methanol extract (LC 50 = 5.13 μg/mL) was slightly more toxic than the peptide extract (LC 50 = 6.12 μg/mL).Conclusions: This is the first report on detection of bioactive LMW peptides in Mimosaceae family. These peptides appear to be rich in arginine and aromatic amino acids. The peptide extract, in its pre-purified form showed a lower Brine shrimp cytotoxicity and an enhanced antimicrobial activity against the tested gram-negative and grampositive bacteria.

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“…The chemoprotective effect of various polyphenolic compounds has been widely reported [ 29‐33 ] against cellular injury. Polyphenol confers protective benefits against chemical damage to cells, [ 34‐36 ] disease onsets such as cancer, [ 37,38 ] and neurological syndromes.…”

Section: Introductionmentioning

confidence: 99%

Rutin ameliorates copper sulfate‐induced brain damage via antioxidative and anti‐inflammatory activities in rats

Arowoogun

Akanni

Adefisan

et al. 2020

J Biochem & Molecular Tox

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Excessive exposure to Copper (Cu) may result in Cu toxicity and adversely affect health outcomes. We investigated the protective role of rutin on Cu‐induced brain damage. Experimental rats were treated as follows: group I: control; group II: Cu‐sulfate: 200 mg/kg; group III: Cu‐sulfate, and rutin 100 mg/kg; and group IV: rutin 100 mg/kg, for 7 weeks. Cu only treatment significantly decreased body weight gain, while rutin cotreatment reversed this decrease. Cu treatment increased malondialdehyde, nitric oxide level, and myeloperoxidase activity and decreased superoxide dismutase and catalase activities in rat brain. Immunohistochemistry showed that COX‐2, iNOS, and Bcl‐2 proteins were strongly expressed, while Bax was mildly expressed in the brain of Cu‐treated rats. Furthermore, brain histology revealed degenerated neurons, and perforated laminae of cerebral cortex in the Cu‐only treated rats. Interestingly, coadministration of Cu and rutin reduced the observed histological alteration, improved inflammatory and antioxidant biomarkers, thereby protecting against Cu‐induced brain damage via antioxidative and anti‐inflammatory mechanisms.

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Root bark extract of Calliandra portoricensis (Jacq.) Benth. chemoprevents N-methyl-N-nitrosourea-induced mammary gland toxicity in rats

Cited by 12 publications

References 46 publications

Calliandra portoricensisameliorates ovarian and uterine oxido-inflammatory responses inN-methyl-N-nitrosourea and benzo[a]pyrene-treated rats

Calliandra portoricensisameliorates ovarian and uterine oxido-inflammatory responses inN-methyl-N-nitrosourea and benzo[a]pyrene-treated rats

Evaluation of peptide-rich root extracts of Calliandria portoriscensis (Jacq.) Benth (Mimosaceae) for in vitro antimicrobial activity and brine shrimp lethality

Rutin ameliorates copper sulfate‐induced brain damage via antioxidative and anti‐inflammatory activities in rats

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