Root-applied brassinolide can alleviate the NaCl injuries on cotton

Abstract

Brassinolide (BL) is a plant growth regulator. This study analyzes the effects of BL on cotton growth, Na ? accumulation, proline content, MDA content, antioxidant enzyme activities, and differentially expressed genes (DEGs) of cotton roots under NaCl stress. We grew two cotton cultivars, Sumian 12 (salt sensitive) and Sumian 22 (salt tolerant), in continuously aerated Hoagland's nutrient solution supplemented with 200 mM NaCl. NaCl stress increased the Na ? , proline, and MDA content and decreased root activity and protein content in the roots of Sumian 12 and 22, and the change rang of Sumian 12 was higher than that of Sumian 22. The application of BL counteracted the NaCl stress-induced growth inhibition in the two tested cotton cultivars. It reduced the accumulation of Na ? , enhanced proline content, and resulted in an overall change in the activities of antioxidant enzymes causing a decrease in the MDA content of NaCl-stressed roots, and the influence of BL on salt-stressed Sumian 12 plants was more pronounced than that on Sumian 22. The digital gene expression analysis in Sumian 12 indicated that BL application significantly influenced the gene expression in NaCl-stressed roots. The majority of 7659 (3661 up-regulated/3998 down-regulated) DEGs of NaCl/ CK in roots of Sumian 12 were regulated by BL, and the gene expression pattern as a result of the root-applied BL on NaCl-stressed cotton treatment (BL ? NaCl) was similar to the control. Our results indicate that the rootapplied brassinolide alleviates NaCl stress on cotton through improving root activity, physiology, and gene expression.

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

“…In this manner, DPI, EBL and Pro, particularly Pro, lower the ROS in As-stressed tissues. This is in accordance with the reports of Ye et al (2012), Shu et al (2015), Singh et al (2015) and Chandrakar et al (2017a) under different abiotic stresses. Arsenic-impelled alterations in lipid moieties of Glycine max L. were analyzed by measuring contents of both MDA and HNE.…”

“…It has been suggested that DPI directly scavenges ROS or decelerates the ROS generation process via inhibiting O 2˙ˉ synthase enzyme (Ye et al 2012). Similarly, EBL and Pro provide tolerance to oxidative stress either by accumulating endogenous osmolytes or activating antioxidant defense mechanism or both in the stressed tissues (Agami 2014, Shu et al 2015. In this manner, DPI, EBL and Pro, particularly Pro, lower the ROS in As-stressed tissues.…”

Brassinolide Increases Potato Root GrowthIn Vitroin a Dose-Dependent Way and Alleviates Salinity Stress

“…These effects are mediated by BR-induced genes, including defense genes and genes that can detoxify ROS produced in plants experiencing abiotic stress [ 15 ]. Exogenous BR application enhanced salinity stress tolerance enhancement in a variety of crop species [ 14 , 20 ]. Treatment with 24-epibrassinolide (EBL) reduced the salt-stress-induced inhibition of seed germination in Arabidopsis thaliana , Brassica napus , and Cucumis sativus [ 21 , 22 ].…”

“…Since in vitro culture systems have been successfully applied to produce virus-free seed potatoes, they could also be used to screen for salt tolerance. Although many studies demonstrated a positive effect of BRs application on plant tolerance to salt stresses [ 20 , 28 , 29 ], BRs stimulate root growth at low concentrations but are inhibitory at higher concentrations [ 30 – 32 ]. The roles of BRs, however, in adventitious root growth are rarely understood in tissue culture systems.…”

Abiotic Stress Alleviation with Brassinosteroids in Plant Roots

“…Morphological, biochemical, and molecular analyses have been performed to analyse the effects of BRs. However, detailed analyses should be performed to investigate the relationship between abiotic stresses and BRs, especially gene expression studies will provide knowledge about interaction at molecular level in plants [152]. We tried to cite as many papers as possible.…”