Rolling circle amplification and graphene-based sensor-on-a-chip for sensitive detection of serum circulating miRNAs

Treerattrakoon, Kiatnida; Jiemsakul, Thanakorn; Tansarawiput, Chookiat; Pinpradup, Preedee; Iempridee, Tawin; Luksirikul, Patraporn; Khoothiam, Krissana; Dharakul, Tararaj; Japrung, Deanpen

doi:10.1016/j.ab.2019.04.016

Cited by 25 publications

(15 citation statements)

References 29 publications

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“…This result can be used to explain why the optimum concentrations of GRA and DNA probes must be obtained before the actual experiment can be performed in many previous studies. 27,37 The presence of multiple miR-29a chains covering a GRA nanosheet allows more miR−DNA interactions. Both DNA1 and DNA2 in 4R4D form a permanent interaction network with miR-29a, but DNA2 shows tighter binding (Figure 4B).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Therefore, in this work, the addition of complementary DNA probes into the miR–GQD solution is studied to explore the possibility of detecting GRA-adhered miR-29a by probes. DNA probes in this study were successfully employed to detect miR-29a in a previous experimental work (Figure A). The folded DNA is used here to mimic the behavior of native DNA in a miR–GQD solution.…”

Section: Introductionmentioning

confidence: 99%

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What Happens When a Complementary DNA Meets miR-29a Cancer Biomarker in Complex with a Graphene Quantum Dot

Kuntip

Japrung

Pongprayoon

2021

ACS Appl. Bio Mater.

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MicroRNAs (miRNAs), short single-stranded noncoding RNA molecules, serve as potential cancer biomarkers due to their involvement in cancer development. One of the strategies to extract miRNAs is to perform the miRNA adsorption on nanomaterials and dissociation by a complementary DNA strand (DNA probe). Recently, graphene quantum dots (GQDs) were found to show a good ability to absorb miRNAs. Thus, in this work, the mechanism of the GQD-adhered miRNA capture by its complementary DNA is revealed using molecular dynamics simulations. miR-29a, a potential cancer biomarker, is used as a miRNA model. Three systems containing one and four chains of miR-29a in addition to one and four complementary DNA probes (1R1D, 1R4D, and 4R4D) were studied. GQDs are the prime targets of a DNA attack. The full coverage of GQDs is required to protect the adsorption of DNA probes on the GQD face. The nucleobase−backbone interactions are the main contributors to miR−DNA interactions in this work. The interbase paring becomes small because most nucleobases of miR-29a and their probe are stacked to maintain their secondary structures, and some are absorbed on the GQD surface. Apparently, weakening of the nucleobase−GQD π−π stacking and the intrabase-pairing strength is needed for extracting miR-29a by a probe. Although no GQD-absorbed miR-29a desorption is found here, the basic principles obtained can be useful for further utilization of GQDs and their derivatives for miRNA extraction and detection.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

What Happens When a Complementary DNA Meets miR-29a Cancer Biomarker in Complex with a Graphene Quantum Dot

Kuntip

Japrung

Pongprayoon

2021

ACS Appl. Bio Mater.

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“…Two fluorescence-labelled circular ssDNA tags were added to the system for the detection of miR-29a and miR-144, which are particularly important since they are highly expressed in some patients with cancer. The resulting platform showed high sensitivity and selectivity, with a LOD of 0.05 pmol, and was able to detect multiplex miRNAs from both human serum and HeLa cells [ 33 ].…”

Section: Outcomementioning

confidence: 99%

Role of Nano-miRNAs in Diagnostics and Therapeutics

Coradduzza

Bellu

Congiargiu

et al. 2022

IJMS

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MicroRNAs (miRNA) are key regulators of gene expression, controlling different biological processes such as cellular development, differentiation, proliferation, metabolism, and apoptosis. The relationships between miRNA expression and the onset and progression of different diseases, such as tumours, cardiovascular and rheumatic diseases, and neurological disorders, are well known. A nanotechnology-based approach could match miRNA delivery and detection to move beyond the proof-of-concept stage. Different kinds of nanotechnologies can have a major impact on the diagnosis and treatment of miRNA-related diseases such as cancer. Developing novel methodologies aimed at clinical practice represents a big challenge for the early diagnosis of specific diseases. Within this context, nanotechnology represents a wide emerging area at the forefront of research over the last two decades, whose potential has yet to be fully attained. Nanomedicine, derived from nanotechnology, can exploit the unique properties of nanometer-sized particles for diagnostic and therapeutic purposes. Through nanomedicine, specific treatment to counteract only cancer-cell proliferation will be improved, while leaving healthy cells intact. In this review, we dissect the properties of different nanocarriers and their roles in the early detection and treatment of cancer.

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“…As shown in Table S2 (ESI †), the detection efficiency, detection range, LOD, and other performance metrics displayed in our approach were better than others. [18][19][20][21][22][23][24][25][26][27] Without a doubt, this ultrasensitive detection threshold can be ascribed to following reasons: (i) the adoption of the self-quenching fluorescence probes makes detective signal-tobackground ratio greatly improved. Thus, the targets at lower concentrations can be easily detected; (ii) only two kinds of probes are employed in the system, the occurrence of non-specific amplification is avoided, and the exponential growth of products and signals is also guaranteed.…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 99%

A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs

Zhao

et al. 2021

Chem. Commun.

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Rolling circle amplification and graphene-based sensor-on-a-chip for sensitive detection of serum circulating miRNAs

Cited by 25 publications

References 29 publications

What Happens When a Complementary DNA Meets miR-29a Cancer Biomarker in Complex with a Graphene Quantum Dot

What Happens When a Complementary DNA Meets miR-29a Cancer Biomarker in Complex with a Graphene Quantum Dot

Role of Nano-miRNAs in Diagnostics and Therapeutics

A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs

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