Roll, adhere, spread and contract: Structural mechanics of platelet function

Sorrentino, Simona; Studt, Jan‐Dirk; Medalia, Ohad; Sapra, K. Tanuj

doi:10.1016/j.ejcb.2015.01.001

Cited by 60 publications

(51 citation statements)

References 123 publications

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“…However, VWF is highly dynamic in the bloodstream, and at high shear rates (above a threshold of 5000 s −1 ), globular-shaped VWF rapidly unfolds and elongates into a long-chain conformation, changing its functional status from inactive to highly reactive [11]. VWF interacts with platelets via two receptors: glycoprotein (Gp) Ib-IX-V complex and integrin α IIb β 3 [12].…”

Section: Pathophysiology Of Vwf In the Bloodstreammentioning

confidence: 99%

The Role of von Willebrand Factor in Vascular Inflammation: From Pathogenesis to Targeted Therapy

Gragnano

Sperlongano

Golia

et al. 2017

Mediators of Inflammation

174

162

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Beyond its role in hemostasis, von Willebrand factor (VWF) is an emerging mediator of vascular inflammation. Recent studies highlight the involvement of VWF and its regulator, ADAMTS13, in mechanisms that underlie vascular inflammation and immunothrombosis, like leukocyte rolling, adhesion, and extravasation; vascular permeability; ischemia/reperfusion injury; complements activation; and NETosis. The VWF/ADAMTS13 axis is implicated in the pathogenesis of atherosclerosis, promoting plaque formation and inflammation through macrophage and neutrophil recruitment in inflamed lesions. Moreover, VWF and ADAMTS13 have been recently proposed as prognostic biomarkers in cardiovascular, metabolic, and inflammatory diseases, such as diabetes, stroke, myocardial infarction, and sepsis. All these features make VWF an attractive therapeutic target in thromboinflammation. Several lines of research have recently investigated “tailor-made” inhibitors of VWF. Results from animal models and clinical studies support the potent anti-inflammatory and antithrombotic effect of VWF antagonism, providing reassuring data on its safety profile. This review describes the role of VWF in vascular inflammation “from bench to bedside” and provides an updated overview of the drugs that can directly interfere with the VWF/ADAMTS13 axis.

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Section: Pathophysiology Of Vwf In the Bloodstreammentioning

confidence: 99%

The Role of von Willebrand Factor in Vascular Inflammation: From Pathogenesis to Targeted Therapy

Gragnano

Sperlongano

Golia

et al. 2017

Mediators of Inflammation

174

162

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“…Platelet biology is another field that is benefiting from structural studies using EM (reviewed in Sorrentino et al, 2015). Recently, Wang et al have illustrated how cryo-ET can be utilized as a noninvasive method to detect structural biomarkers of diseases that affect platelets (Wang et al, 2015).…”

Section: Cryo-et As a Medical Diagnostics Toolmentioning

confidence: 99%

Cellular structural biology as revealed by cryo-electron tomography

Irobalieva

Martins

Medalia

2016

Journal of Cell Science

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Understanding the function of cellular machines requires a thorough analysis of the structural elements that underline their function. Electron microscopy (EM) has been pivotal in providing information about cellular ultrastructure, as well as macromolecular organization. Biological materials can be physically fixed by vitrification and imaged with cryo-electron tomography (cryo-ET) in a close-to-native condition. Using this technique, one can acquire three-dimensional (3D) information about the macromolecular architecture of cells, depict unique cellular states and reconstruct molecular networks. Technical advances over the last few years, such as improved sample preparation and electron detection methods, have been instrumental in obtaining data with unprecedented structural details. This presents an exciting opportunity to explore the molecular architecture of both individual cells and multicellular organisms at nanometer to subnanometer resolution. In this Commentary, we focus on the recent developments and in situ applications of cryo-ET to cell and structural biology.

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“…They transmit signals to and from cells, thus sensing the environment and controlling cell shape and motility of cells (5). Specific integrins mediate the interaction of platelets with other platelets or other cell types, including endothelial cells and cancer cells.…”

Section: Direct Cell-cell Interaction and Autacoid Release In Health mentioning

confidence: 99%

“…Specific integrins mediate the interaction of platelets with other platelets or other cell types, including endothelial cells and cancer cells. Integrins mediate platelet adhesion and aggregation two phenomena involved in the maintenance of the integrity of the vasculature (5).…”

Section: Direct Cell-cell Interaction and Autacoid Release In Health mentioning

confidence: 99%