Roles of the His-Asp Phosphorelay Signal Transduction System in Controlling Cell Growth and Development in<i>Aspergillus nidulans</i>

Kanamaru, Kyoko

doi:10.1271/bbb.100551

Cited by 9 publications

(8 citation statements)

References 34 publications

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“…2 A) as reported previously [9]. PhkA and PhkB are thought to play roles in oxidative stress responses in A. nidulans because they have functional domains similar to those of Phk1–3, which are histidine kinases that function in response to oxidative stresses in Schizosaccharomyces pombe [1]. However, both ΔphkA and ΔphkB exhibited the same sensitivity to oxidative stress as the wild‐type (Fig.…”

Section: Resultssupporting

confidence: 75%

“…(E) Strains were cultivated as in (A) and (B), except the restricted oxygen condition, in which oxygen restricted by taping around the plates. ΔphkB Tip -T i p Oxidative stress response [ 1 ] ΔnikA Hypha Hypha Hypha Fungicide sensitivity [ 4 , 5 ] ΔhysA Hypha Hypha Conidia Tip Hypoxia response [ 1 ] ΔnapA Hypha -H y p h a Transcription factor of oxidative stress response [ 11 ] most HKs were expressed with the progression of asexual development, even though their overall expression levels were low ( Fig. 1 B).…”

Section: Expression Levels Of Hk Proteins In a Nidulansmentioning

confidence: 99%

“…Finally, response regulators (RRs) receive the phosphate signals on their aspartic acid residues and regulate gene expressions directly or by controlling the downstream signal transduction pathways. Aspergillus nidulans is a model filamentous fungus that contains 15 HKs, 1 HPt, and 4 RRs [ 1 ]. Several HKs have been studied in attempts to characterize the roles of His-Asp phosphorelay systems in A. nidulans .…”

Section: Introductionmentioning

confidence: 99%

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Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

Hayashi¹,

Yoshioka²,

Matsui³

et al. 2014

FEBS Open Bio

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Sensor histidine kinases (HKs) are important factors that control cellular growth in response to environmental conditions. The expression of 15 HKs from Aspergillus nidulans was analyzed by quantitative real-time PCR under vegetative, asexual, and sexual growth conditions. Most HKs were highly expressed during asexual growth. All HK gene-disrupted strains produced reactive oxygen species (ROS). Three HKs are involved in the control of ROS: HysA was the most abundant under the restricted oxygen condition, NikA is involved in fungicide sensing, and FphA inhibits sexual development in response to red light. Phosphotransfer signal transduction via HysA is essential for ROS production control.

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Section: Resultssupporting

confidence: 75%

Section: Expression Levels Of Hk Proteins In a Nidulansmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

Hayashi¹,

Yoshioka²,

Matsui³

et al. 2014

FEBS Open Bio

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“…3). Fifteen genes encoding receptor histidine kinases are present in the A. nidulans genome (Kanamaru, 2011) and four of them (phkB, hk-8-1, hk-8-2 and hk-8-3) are highly induced in all three 9 mutants, and three others (tcsB, hk2, and hk-8-5) in the sconC mutant only. Because the gene coding for the single histidine-containing phosphotransfer protein YpdA is also up-regulated (Supplementary Table 2), this indicates that the whole two-component signal transduction pathway is stimulated in the mutants.…”

Section: Genes Involved In Signal Transductionmentioning

confidence: 99%

Regulatory mutations affecting sulfur metabolism induce environmental stress response in Aspergillus nidulans

Sieńko¹,

Natorff²,

Skoneczny³

et al. 2014

Fungal Genetics and Biology

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Mutations in the cysB, sconB and sconC genes affect sulfur metabolism in Aspergillus nidulans in different ways. The cysB mutation blocks synthesis of cysteine by the main pathway and leads to a shortage of this amino acid. The sconB and sconC mutations affect subunits of the SCF ubiquitin ligase complex, which inactivates the MetR transcription factor in the presence of an excess of cysteine. In effect, both cysB and scon mutations lead to permanent derepression of MetR-dependent genes. We compared transcriptomes of these three mutants with that of a wild type strain finding altered expression of a few hundred genes belonging to various functional categories. Besides those involved in sulfur metabolism, many up-regulated genes are related to stress responses including heat shock and osmotic stress.However, only the scon strains are more resistant to exogenous stress agents than the wild type strain while cysB is more sensitive. The two-component signal transduction system is a functional category, which is most enriched among genes up-regulated in the cysB, sconB and sconC mutants. A large group of up-regulated genes are involved in carbohydrate and energy metabolism, including genes coding for enzymes of trehalose and glycerol synthesis. The altered expression of these genes is accompanied by changes in sugar and polyol accumulation in conidia of the mutants. Genes encoding enzymes of the glyoxylate bypass and the GABA shunt are also up-regulated along with genes coding for enzymes of alcohol fermentation. Among the down-regulated genes the most numerous are those encoding membrane proteins and enzymes involved in secondary metabolism, including the penicillin biosynthesis cluster.

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“…This raises the question of whether as many as 21 signals sensed by the 21 HHKs, which is the case of Cochliobolus heterostrophus, are integrated into a single output response, or whether sensing of a signal by a specific HHK triggers a specific output response. In this respect it is important to mention that the thus far studied fungal RRs and HHKs not only present phenotypes related to osmolarity and oxidative stress resistance (Hagiwara et al, 2009;Motoyama et al, 2005;Noguchi et al, 2007;Schumacher et al, 1997;Vargas-P erez et al, 2007;Yoshimi et al, 2005), similar to those of S. cerevisiae, but have also been shown to be involved in other physiological processes such as sexual or asexual reproduction, pigmentation, virulence, fungicide resistance, normal sporulation, and conidiospore viability (Barba-Ostria et al, 2011;Boyce et al, 2011;Hagiwara et al, 2013;Kanamaru, 2011;Oide et al, 2010;Vargas-P erez et al, 2007;Zhang et al, 2010). Thus, some signal/output specificity should be present in these systems.…”

Section: Obligatory Branched Pathways In Fungimentioning

confidence: 99%

Organization and mode of action of two component system signaling circuits from the various kingdoms of life

Álvarez

Barba-Ostria

Silva-Jiménez

et al. 2016

Environmental Microbiology

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Two-component system (TCS) signaling circuits regulate numerous cellular processes in response to environmental cues in both prokaryotic and eukaryotic cells. These signaling circuits are all based on phosphoryl-group transfers between histidine and aspartate containing modules of sensor kinase and response regulator proteins. Curiously, the architecture and organization of prokaryotic and eukaryotic two-component systems reveal notable variations, raising the question of whether the input-response specificity that governs the majority of prokaryotic TCSs also governs the eukaryotic ones. In this review, we contrast the TCS architecture and signaling circuits of prokaryotes and eukaryotes, and discuss their possible consequences on signaling specificity.

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Roles of the His-Asp Phosphorelay Signal Transduction System in Controlling Cell Growth and Development inAspergillus nidulans

Cited by 9 publications

References 34 publications

Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

Regulatory mutations affecting sulfur metabolism induce environmental stress response in Aspergillus nidulans

Organization and mode of action of two component system signaling circuits from the various kingdoms of life

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