Control of reactive oxygen species (ROS) production through histidine kinases in <i>Aspergillus nidulans</i> under different growth conditions

Hayashi, Saki; Yoshioka, Megumi; Matsui, Tetsuji; Kojima, Kensuke; Kato, Masashi; Kanamaru, Kyoko; Kobayashi, Tetsuo

doi:10.1016/j.fob.2014.01.003

Cited by 15 publications

(12 citation statements)

References 15 publications

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“…Among the above-mentioned proteins, the involvement of MpkC in the cell wall and osmotic stress responses of A. fumigatus ( Pereira Silva et al. 2017 ) and the contribution of NikA to the regulation of oxidative stress response in A. nidulans ( Hayashi et al. 2014 ) have been experimentally verified.…”

Section: Resultsmentioning

confidence: 99%

Duplications and losses of genes encoding known elements of the stress defence system of the Aspergilli contribute to the evolution of these filamentous fungi but do not directly influence their environmental stress tolerance

Emri¹,

Antal²,

Riley³

et al. 2018

Studies in Mycology

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The contribution of stress protein duplication and deletion events to the evolution of the Aspergilli was studied. We performed a large-scale homology analysis of stress proteins and generated and analysed three stress defence system models based on Saccharomyces cerevisiae, Schizosaccharomyces pombe and Aspergillus nidulans. Although both yeast-based and A. nidulans-based models were suitable to trace evolutionary changes, the A. nidulans-based model performed better in mapping stress protein radiations. The strong Mantel correlation found between the positions of species in the phylogenetic tree on the one hand and either in the A. nidulans-based or S. cerevisiae-based models on the other hand demonstrated that stress protein expansions and reductions contributed significantly to the evolution of the Aspergilli. Interestingly, stress tolerance attributes correlated well with the number of orthologs only for a few stress proteins. Notable examples are Ftr1 iron permease and Fet3 ferro-O2-oxidoreductase, elements of the reductive iron assimilation pathway, in the S. cerevisiae-based model, as well as MpkC, a HogA-like mitogen activated protein kinase in the A. nidulans-based model. In the case of the iron assimilation proteins, the number of orthologs showed a positive correlation with H2O2-induced stress tolerance while the number of MpkC orthologs correlated positively with Congo Red induced cell wall stress, sorbitol induced osmotic stress and H2O2 induced oxidative stress tolerances. For most stress proteins, changes in the number of orthologs did not correlate well with any stress tolerance attributes. As a consequence, stress tolerance patterns of the studied Aspergilli did not correlate with either the sets of stress response proteins in general or with the phylogeny of the species studied. These observations suggest that stress protein duplication and deletion events significantly contributed to the evolution of stress tolerance attributes of Aspergilli. In contrast, there are other processes, which may counterbalance the effects of stress gene duplications or deletions including (i) alterations in the structures of stress proteins leading to changes in their biological activities, (ii) varying biosynthesis of stress proteins, (iii) rewiring stress response regulatory networks or even (iv) acquiring new stress response genes by horizontal gene transfer. All these multilevel changes are indispensable for the successful adaptation of filamentous fungi to altering environmental conditions, especially when these organisms are entering new ecological niches.

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Section: Resultsmentioning

confidence: 99%

Duplications and losses of genes encoding known elements of the stress defence system of the Aspergilli contribute to the evolution of these filamentous fungi but do not directly influence their environmental stress tolerance

Emri¹,

Antal²,

Riley³

et al. 2018

Studies in Mycology

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“…The hysA deletion strain (ΔhysA) was previously constructed by replacing the hysA gene of the ABPU1 strain with the wild-type argB gene (Hayashi et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work, all deletion strains of HKs were studied by staining with nitroblue tetrazolium (NBT), to identify signal transduction systems controlling ROS production (Hayashi et al, 2014). In this report, one of these HKs, HysA (AN4113), which showed intense staining with NBT in the deletion strain, was characterized.…”

Section: Introductionmentioning

confidence: 99%

Generation of mitochondrial reactive oxygen species through a histidine kinase, HysA in <i>Aspergillus nidulans</i>

Kanamaru

Izuhara

Kimura

et al. 2022

J. Gen. Appl. Microbiol.

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The His-Asp phosphorelay signal transduction from histidine kinase (HK) to the response regulator (RR) is an important mechanism for adaptation to environmental changes. Aspergillus nidulans expresses 15 different HKs, which may be involved in different types of adaptations. As reactive oxygen species (ROS) are a key signal of environmental changes, some HKs might be involved in ROS generation through transcriptional regulation. Previously, we identified 3 HK (NikA, FphA, and HysA) deletion strains that showed increased ROS production during growth. We also showed that the phosphorylation function of HysA is involved in ROS generation. Here, we investigated the role of HysA in ROS production in A. nidulans cells. HysA protein was detected in both the cytosol and mitochondria by biological fractionation of the vegetative cell lysate of A. nidulans. The subcellular localization analysis by expressing the the HysA-GFP fusion protein along with MitoTracker Red staining did not clearly reveal mitochondrial localization of HysA at the conidiophore during asexual development. However, mitochondrial ROS in hysA mutant strains were detected by MitoSOX Red staining, and their excess levels possibly caused morphological changes during asexual development.

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“…33) However, the growth of the deletion mutants of phkA or phkB genes under high osmolarity stress conditions was comparable to that of the wild-type (WT) A. fumigatus strain. 33) In both A. fumigatus and A. nidulans, 60) the phkA and phkB deletion mutants did not show reduced growth under oxidative stress conditions, compared with that of the parental strains. The physiological roles of the HKs in Aspergillus species remain unknown.…”

Section: III Tcs Systemmentioning

confidence: 96%

“…This was partly proven by comprehensive transcriptional analyses of an array of HKs expressed at various developmental stages and during stress responses in both A. nidulans and A. fumgiatus. For instance, A. nidulans nikA was upregulated during the asexual stage and preferentially expressed in conidial heads and stalks 60,73) ; hysA was upregulated in response to osmotic shock and fludioxonil treatment and was expressed in conidia 73,74) ; A. fumigatus fos-1, phkA, phkB, and fhk6 were upregulated in response to osmotic shock 33) ; A. fumigatus fhk1 was upregulated during the asexual stage 37) ; and A. fumigatus fhk5 was upregulated under oxygen-limited conditions. 75) The HPt is found in all fungal species, which highlights its importance in the TCS system.…”

Section: III Tcs Systemmentioning

confidence: 99%

Signaling pathways for stress responses and adaptation in Aspergillus species: stress biology in the post-genomic era

Hagiwara

Sakamoto

Abe

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Aspergillus species are among the most important filamentous fungi in terms of industrial use and because of their pathogenic or toxin-producing features. The genomes of several Aspergillus species have become publicly available in this decade, and genomic analyses have contributed to an integrated understanding of fungal biology. Stress responses and adaptation mechanisms have been intensively investigated using the accessible genome infrastructure. Mitogen-activated protein kinase (MAPK) cascades have been highlighted as being fundamentally important in fungal adaptation to a wide range of stress conditions. Reverse genetics analyses have uncovered the roles of MAPK pathways in osmotic stress, cell wall stress, development, secondary metabolite production, and conidia stress resistance. This review summarizes the current knowledge on the stress biology of Aspergillus species, illuminating what we have learned from the genomic data in this "post-genomic era."

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Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

Cited by 15 publications

References 15 publications

Duplications and losses of genes encoding known elements of the stress defence system of the Aspergilli contribute to the evolution of these filamentous fungi but do not directly influence their environmental stress tolerance

Duplications and losses of genes encoding known elements of the stress defence system of the Aspergilli contribute to the evolution of these filamentous fungi but do not directly influence their environmental stress tolerance

Generation of mitochondrial reactive oxygen species through a histidine kinase, HysA in <i>Aspergillus nidulans</i>

Signaling pathways for stress responses and adaptation in Aspergillus species: stress biology in the post-genomic era

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