Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Tzima, Aliki; Paplomatas, Epaminondas J.; Rauyaree, Payungsak; Kang, Seogchan

doi:10.1016/j.fgb.2010.01.007

Cited by 90 publications

(93 citation statements)

References 59 publications

(108 reference statements)

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“…The occlusion can be due to a physical blockage of the xylem by the pathogen or due to host defense responses that result in vessel plugging (Fradin and Thomma 2006). In recent years, some genes related to V. dahliae pathogenicity have been identified, such as a mitogen-activated protein kinase (VMK1) (Rauyaree et al 2005), a cAMP-dependent protein kinase A (VdPKAC1) (Tzima et al 2010), a glutamic acid-rich protein (VdGARP1) (Gao et al 2010) and a sucrose nonfermenting 1 gene (VdSNF1) (Tzima et al 2011). Proteins that can induce plant cell death have also been isolated from a culture supernatant of V. dahliae such as VdNEP (Wang et al 2004); however, there is still doubt whether any of these play key role in V. dahliae pathogenicity (Zhou et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

Xie

Wang

et al. 2013

J Gen Plant Pathol

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Verticillium dahliae is one of the most destructive soil-borne fungal pathogens that cause vascular wilt diseases in a wide range of important crop plants, including cotton. However, the mechanisms used by this pathogen to infect cotton have not been fully elucidated. In the present study, we first investigated changes in protein abundance during the initial interaction between cotton roots and V. dahliae. Among the proteins that were upregulated upon infection, some were related to reactive oxygen species (ROS); among those downregulated upon infection were proteins involved in normal metabolism or cell structure. Further experiments confirmed that a sudden release of ROS and cell death accompany V. dahliae infection in the cotton vasculature. Further analysis indicated that a culture supernatant of V. dahliae induced lesion formation in tobacco leaves; de novo protein synthesis not active gene expression was required for this induction. Lesion formation was dependent on the age of leaves, but neither the known ROS burst nor the ubiquitin/ 26S proteasome system are prerequisites.

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Section: Introductionmentioning

confidence: 99%

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

Xie

Wang

et al. 2013

J Gen Plant Pathol

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“…Although microsclerotium development is not always coupled with virulence (Klimes and Dobinson 2006;Tzima et al 2010), some studies have uncovered evidence that a decrease in microsclerotia and pigmentation reduces survival and pathogenicity of V. dahliae isolates (Hawke and Lazarovits 1994;Coley-Smith and Cooke 1971). Germination of microsclerotia is the first step in host invasion and initiation of wilt disease.…”

Section: Discussionmentioning

confidence: 99%

Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton

Zhang

Feng

et al. 2015

Curr Genet

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The fungal plant pathogen Verticillium dahliae is the causal agent of vascular wilt, a disease that can seriously diminish cotton fiber yield. The pathogenicity mechanism and the identity of the genes that interact with cotton during the infection process still remain unclear. In this study, we investigated the low-pathogenic, non-microsclerotium-producing mutant vdpr3 obtained in a previous study from the screening of a T-DNA insertional library of the highly virulent isolate Vd080; the pathogenicity-related gene (VdPR3) in wild-type strain Vd080 was cloned. Knockout mutants (ΔVdPR3) showed lower mycelium growth and obvious reduction in sporulation ability without microsclerotium formation. An evaluation of carbon utilization in mutants and wild-type isolate Vd080 demonstrated that mutants-lacking VdPR3 exhibited decreased cellulase and amylase activities, which was restored in the complementary mutants (ΔVdPR3-C) to levels similar to those of Vd080. ΔVdPR3 postponed infectious events in cotton and showed a significant reduction in pathogenicity. Reintroduction of a functional VdPR3 copy into ΔVdPR3-C restored the ability to infect cotton plants. These results suggest that VdPR3 is a multifunctional gene involved in growth development, extracellular enzyme activity, and virulence of V. dahliae on cotton.

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“…Agrobacterium tumefaciens mediated transformation (ATMT) of 70wt-r1 using AGL1 carrying pGGbGen was performed as described previously (Mullins et al, 2001;Tzima et al, 2010Tzima et al, , 2011. For complementation, a 2186 bp DNA fragment (submitted in GenBank with Accession No.…”

Section: Isolation Of the Vgb Gene Vector Construction And Fungal Trmentioning

confidence: 99%

“…Ethylene production by 70DGb15, 70DGbPKAC1 and 70wt-r1 was measured as described previously (Tzima et al, 2010).…”

Section: Ethylene Productionmentioning

confidence: 99%

“…Gene specific primers used were: VGB (Vgb_q-up: 5 0 -CGG TGC CTG CGA TGC TTT-3 0 , Vgb_q-dn: 5 0 -GGA GGT GAT GCC ACA GAG GA-3 0 ), VMK1 (VMK1_q-up: 5 0 -CGC AGC AAC GCC CCT AAT C-3 0 , VMK1_q-dn: 5 0 -GGC AGT GGT CAT CGG AGA GGT-3) and ACS1 (ACS1_D-TN_q-up: 5 0 -GGG GGC GCT CAT AAC CAA GAA T-3 0 , ACS1_D-TN_q-dn: 5 0 -CCC CCG GAT GCA AGA AAA CC-3 0 ). Transcripts of VDH1 (Klimes and Dobinson, 2006) and VdPKAC1, VdPKAC2 (Tzima et al, 2010) were quantified using primers previously described. PCR reactions were performed in duplicates and for data analysis, average threshold cycle (Ct) values were calculated for each gene of interest on the basis of four independent biological samples (Pfaffl, 2001).…”

Section: Quantitative Real-time Rt-pcr Reactionmentioning

confidence: 99%

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The G protein β subunit controls virulence and multiple growth- and development-related traits in Verticillium dahliae

Tzima

Paplomatas

Tsitsigiannis

et al. 2012

Fungal Genetics and Biology

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Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Cited by 90 publications

References 59 publications

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton

The G protein β subunit controls virulence and multiple growth- and development-related traits in Verticillium dahliae

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