Roles of quorum sensing molecules from Rhizobium etli RT1 in bacterial motility and biofilm formation

Dixit, S. N.; Dubey, R. C.; Maheshwari, Dinesh Kumar; Seth, Prahlad K.; Bajpai, Vivek K.

doi:10.1016/j.bjm.2016.08.005

Cited by 12 publications

(2 citation statements)

References 39 publications

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“…Mangwani, Kumari, and Das () reported that 1–2 μg/mL of C4‐HSL and C8‐HSL could enhance the biofilm growth and swarming motility of Pseudomonas pseudoalcaligenes . Dixit, Dubey, Maheshwari, Seth, and Bajpai () also reported that bacterial motility and biofilm formation were affected or modified on increasing concentrations of 3‐oxo‐C8‐HSL.…”

Section: Resultsmentioning

confidence: 95%

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

et al. 2017

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This study characterized the spoilage potential of the N-acyl homoserine lactone (AHL)-producing strain, Aeromonas veronii bv. veronii, isolated from fermented surimi, and investigated the effects of exogenous AHLs on its spoilage characteristics. The strain showed a strong ability of producing total volatile base nitrogen (TVB-N) and putrescine, and four types of AHLs, including N-butanoyl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-heptanoyl-Lhomoserine lactone (C7-HSL), and N-octanoyl-L-homoserine lactone (C8-HSL), were identified from the supernatant by LC-MS/MS. Concentrations of AHLs reached the maximum value as the cells entered stationary phase contents and then decreased rapidly with the increasing pH. C8-HSL was found to be dominant with contents of 1,536.33 ng/mL and exogenous C8-HSL was most effective in promoting bacterial growth, biofilm formation, swimming motility, production of TVB-N and putrescine, and relative genes expression. The results indicated the involvement of AHL-based quorum sensing system in the regulation of spoilage potential of Aeromonas spp. Practical applicationsThe results of the present study found that Aeromonas veronii bv. veronii was capable of producing four types of AHLs, which were considered to be involved in the regulation of spoilage potential of the strain. This would enrich the understanding of quorum sensing system in Aeromonas and give clue for new strategy of controlling food spoilage in food industry. K E Y W O R D SAeromonas veronii bv. veronii, homoserine lactone, regulation, quorum sensing, surimi, spoilage potential

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Section: Resultsmentioning

confidence: 95%

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

et al. 2017

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“…Marine bacterial strains ST2 from Scrippsiella trochoidea and RT1 from the root nodules of Lens culinaris , have been found to produce multiple AHL compounds, including C4-AHL, C8-AHL, and C10-AHL, and 3-oxo-C8-HSL and 3-OH-C10-HSL, respectively [ 75 ]. Furthermore, 3-oxo-C8-HSL, produced by RT1, has also been found to influence bacterial motility and biofilm formation [ 82 ]. Additionally, the bacterium Anemonia viridis , isolated from cnidarian species, has been reported to produce five AHLs: C6-HSL, C8-HSL, 3-OH-C6-HSL, 3-OH-C8-HSL, and 3-OH-C10-HSL [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in Porphyra haitanensis-Associated Pseudoalteromonas galatheae

Aslam,

Pei,

et al. 2023

Microorganisms

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N-acyl homoserine lactones (AHLs) are small, diffusible chemical signal molecules that serve as social interaction tools for bacteria, enabling them to synchronize their collective actions in a density-dependent manner through quorum sensing (QS). The QS activity from epiphytic bacteria of the red macroalgae Porphyra haitanensis, along with its involvement in biofilm formation and regulation, remains unexplored in prior scientific inquiries. Therefore, this study explores the AHL signal molecules produced by epiphytic bacteria. The bacterium isolated from the surface of P. haitanensis was identified as Pseudoalteromonas galatheae by 16s rRNA gene sequencing and screened for AHLs using two AHL reporter strains, Agrobacterium tumefaciens A136 and Chromobacterium violaceum CV026. The crystal violet assay was used for the biofilm-forming phenotype. The inferences revealed that P. galatheae produces four different types of AHL molecules, i.e., C4-HSL, C8-HSL, C18-HSL, and 3-oxo-C16-HSL, and it was observed that its biofilm formation phenotype is regulated by QS molecules. This is the first study providing insights into the QS activity, diverse AHL profile, and regulatory mechanisms that govern the biofilm formation phenotype of P. galatheae. These findings offer valuable insights for future investigations exploring the role of AHL producing epiphytes and biofilms in the life cycle of P. haitanensis.

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Microbes Living Together: Exploiting the Art for Making Biosurfactants and Biofilms

Quadriya

Ali

Jakinala

et al. 2018

Implication of Quorum Sensing System in Biofilm Formation and Virulence

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Roles of quorum sensing molecules from Rhizobium etli RT1 in bacterial motility and biofilm formation

Cited by 12 publications

References 39 publications

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in Porphyra haitanensis-Associated Pseudoalteromonas galatheae

Microbes Living Together: Exploiting the Art for Making Biosurfactants and Biofilms

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