Microbes Living Together: Exploiting the Art for Making Biosurfactants and Biofilms

Quadriya, Humera; Ali, Saleh; Jakinala, Parameshwar; Manasa, M; Khan, Mahejibin; Hameeda, Bee

doi:10.1007/978-981-13-2429-1_10

Cited by 7 publications

(9 citation statements)

References 93 publications

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“…Rhamonolipids reduce the lipopolysaccharide content in membranes, increase cell hydrophobicity, alter membrane proteins and disturb surface morphology. Additionally, biosurfactants aid in the detachment of microbial cells from surfaces through sloughing, erosion and abrasion [ 45 ]. Furthermore, biosurfactants regulate the quorum sensing signaling (intercellular and intracellular communication) and quorum sensing-dependent activities, such as biofilm formation, motility and pathogenicity, are influenced by this saignaling.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Bacterial Adhesion and Antibiofilm Activities of a Glycolipid Biosurfactant from Lactobacillus rhamnosus with Its Physicochemical and Functional Properties

et al. 2021

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Biosurfactants derived from different microbes are an alternative to chemical surfactants, which have broad applications in food, oil, biodegradation, cosmetic, agriculture, pesticide and medicine/pharmaceutical industries. This is due to their environmentally friendly, biocompatible, biodegradable, effectiveness to work under various environmental conditions and non-toxic nature. Lactic acid bacteria (LAB)-derived glycolipid biosurfactants can play a major role in preventing bacterial attachment, biofilm eradication and related infections in various clinical settings and industries. Hence, it is important to explore and identify the novel molecule/method for the treatment of biofilms of pathogenic bacteria. In the present study, a probiotic Lactobacillus rhamnosus (L. rhamnosus) strain was isolated from human breast milk. Firstly, its ability to produce biosurfactants, and its physicochemical and functional properties (critical micelle concentration (CMC), reduction in surface tension, emulsification index (% EI24), etc.) were evaluated. Secondly, inhibition of bacterial adhesion and biofilm eradication by cell-bound biosurfactants from L. rhamnosus was performed against various biofilm-forming pathogens (B. subtilis, P. aeruginosa, S. aureus and E. coli). Finally, bacterial cell damage, viability of cells within the biofilm, exopolysaccharide (EPS) production and identification of the structural analogues of the crude biosurfactant via gas chromatography–mass spectrometry (GC–MS) analysis were also evaluated. As a result, L. rhamnosus was found to produce 4.32 ± 0.19 g/L biosurfactant that displayed a CMC of 3.0 g/L and reduced the surface tension from 71.12 ± 0.73 mN/m to 41.76 ± 0.60 mN/m. L. rhamnosus cell-bound crude biosurfactant was found to be effective against all the tested bacterial pathogens. It displayed potent anti-adhesion and antibiofilm ability by inhibiting the bacterial attachment to surfaces, leading to the disruption of biofilm formation by altering the integrity and viability of bacterial cells within biofilms. Our results also confirm the ability of the L. rhamnosus cell-bound-derived biosurfactant to damage the architecture of the biofilm matrix, as a result of the reduced total EPS content. Our findings may be further explored as a green alternative/approach to chemically synthesized toxic antibiofilm agents for controlling bacterial adhesion and biofilm eradication.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Bacterial Adhesion and Antibiofilm Activities of a Glycolipid Biosurfactant from Lactobacillus rhamnosus with Its Physicochemical and Functional Properties

et al. 2021

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“…For interspecies communication, C4-HSL, N-butanoylhomoserine lactone, 3-oxo-C12-HSL, peptide lactones, and peptide thiolactones signaling molecules are used (Ng and Bassler, 2009;Quadriya et al, 2018).…”

Section: Biosurfactants and Quorum Sensingmentioning

confidence: 99%

“…Similarly, gamma-butyrolactones, and cyclic peptides are used for communication in case of Gram-positive bacteria. For interspecies communication, C4-HSL, N-butanoylhomoserine lactone, 3-oxo-C12-HSL, peptide lactones, and peptide thiolactones signaling molecules are used ( Ng and Bassler, 2009 ; Quadriya et al, 2018 ).…”

Section: Biosurfactants and Quorum Sensingmentioning

confidence: 99%

“…Biofilms are advantageous in some applications such as bioremediation and waste-water treatment. Biosurfactants help in conditioning of the film and modification of the bacterial surface hydrophobicity, and therefore help in bacterial adhesion to surfaces for biofilm formation ( Quadriya et al, 2018 ). For example, a study demonstrated the influence of lipopeptide biosynthesis on the decreasing cell surface hydrophobicity and on the adhesion of Bacillus spp.…”

Section: Biosurfactant Role In Biofilm Formation and Removalmentioning

confidence: 99%

“…Another study showed the essential role of biosurfactants in bacterial adsorption and biofilm formation at the ƞ-decane–water interface by P. aeruginosa, S. aureus, and S. epidermidis ( Subbiahdoss and Reimhult, 2020 ). Biosurfactants also help in the detachment of microbial cells from the surfaces by erosion, sloughing, and abrasion ( Quadriya et al, 2018 ). Because of this property, they can be used as a strong anti-adhesive agent to prevent microbial infections ( Brindhadevi et al, 2020 ).…”

Section: Biosurfactant Role In Biofilm Formation and Removalmentioning

confidence: 99%

See 2 more Smart Citations

Biosurfactants: Potential Agents for Controlling Cellular Communication, Motility, and Antagonism

Sharma

Sundar

Srivastava

2021

Front. Mol. Biosci.

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Biosurfactants are surface-active molecules produced by microorganisms, either on the cell surface or secreted extracellularly. They form a thin film on the surface of microorganisms and help in their detachment or attachment to other cell surfaces. They are involved in regulating the motility of bacteria and quorum sensing. Here, we describe the various types of biosurfactants produced by microorganisms and their role in controlling motility, antagonism, virulence, and cellular communication.

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Biosurfactants and biomass treatment technologies for heavy metals in wastewater

Mishra,

Kulkarni,

Abrar

et al. 2024

Bio-Organic Amendments for Heavy Metal Remediation

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Microbes Living Together: Exploiting the Art for Making Biosurfactants and Biofilms

Cited by 7 publications

References 93 publications

Inhibition of Bacterial Adhesion and Antibiofilm Activities of a Glycolipid Biosurfactant from Lactobacillus rhamnosus with Its Physicochemical and Functional Properties

Inhibition of Bacterial Adhesion and Antibiofilm Activities of a Glycolipid Biosurfactant from Lactobacillus rhamnosus with Its Physicochemical and Functional Properties

Biosurfactants: Potential Agents for Controlling Cellular Communication, Motility, and Antagonism

Biosurfactants and biomass treatment technologies for heavy metals in wastewater

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