Roles of peripheral B1 cells in the individualized treatment of adult idiopathic thrombocytopenic purpura

Abstract

ABSTRACT. We aimed to explore the changes of peripheral B1 cells before and after treatment of adult idiopathic thrombocytopenic purpura (ITP) and to investigate the association of these changes with the disease condition and prognosis. Ninety-seven ITP patients were divided into the effective or ineffective groups, based on their response to hormone therapy. Forty healthy volunteers were enrolled into the control group (HC). The percentages of CD19 + cells, B1 cells, and platelet-associated immunoglobulin (PAIg) in peripheral blood from healthy volunteers and ITP patients before and after treatment were evaluated, and blood platelet (PLT) counts were determined. The percentages of CD19 + cells [(21 ± 10.0) vs (11.2 ± 7.1)%], B1 cells [(8.85 ± 5.23) vs (2.2 ± 1.3)%], and PAIg [(28 ± 19) vs (11.7 ± 8)%] in whole blood from ITP patients before treatment were significantly higher than those in whole blood from healthy controls (P < 0.05). Before treatment, the percentage of B1 cells and PAIg in ITP patients was negatively correlated with the PLT level (r = -0.89, P < 0.05 and r = -0.814, P < 0.05, respectively). Further, the B1 cell percentage was positively associated with the PAIg percentage in ITP patients before treatment. In the effective group, the B1 cell percentage was reduced sharply at 1 month after treatment [(2.45 ± 1.75) vs (8.74 ± 5.04)%, P < 0.05)], so as at 3 and 6 months. However, in the ineffective group, there was no difference in the B1 cell percentage before and after treatment [(7.9 ± 5.6) vs (8.76 ± 5.26)%]. This obvious association of changes in peripheral B1 cells with disease condition and prognosis in ITP patients may be of certain clinical significance for guiding the individualized treatment of ITP.

Research progress on immune aging and its mechanisms affecting geriatric diseases

“…B1 cells play an important role in autoimmune diseases mediated by antibodies (such as SLE, rheumatoid arthritis, and Graves' disease). 47 The shift of B‐cell subsets to CD5+ B1 in aging individuals may be one of the reasons why the elderly are prone to autoimmune diseases. 41…”

Expression of CD markers' in immune thrombocytopenic purpura: prognostic approaches

“…Although the correlation between the number of CD5 + B1 cells with platelets is not clear in ITP patients, increased number of B1 cells in PB and spleen of these patients has been reported, which is involved in the production of Auto-Abs against platelets in chronic ITP (58). The percentage of B1 cells in PB of ITP patients is directly and inversely associated with Auto-Abs against platelets and platelet number, respectively (57)(58)(59). CD5 + B1 cells have the ability to produce anti-platelet antibodies, a finding indicating that the overexpression of CD5 can be related to disease progression, which is subsequently associated with a poor prognosis in ITP patients.…”