Roles of miR-31 and Endothelin-1 in Psoriasis Vulgaris: Pathophysiological Functions and Potential Biomarkers

Borská, Lenka; Andrýs, Ctirad; Chmelařová, Marcela; Kovaříková, Helena; Krejsek, Jan; Hamáková, Květoslava; Beránek, M.; Palička, Vladimír; Kremláček, Jan; Borský, Pavel; Fiala, Zdeněk

doi:10.33549/physiolres.933615

Cited by 21 publications

(24 citation statements)

References 32 publications

(29 reference statements)

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“…CircRNAs generally exhibit regulatory functions on gene expression through acting as microRNA sponge . miR‐31 are reported to present pro‐inflammatory or anti‐inflammatory activities under different condition . Besides, earlier literature reported that miR‐31 presented anti‐inflammatory activity on lung cystic fibrosis and its expression was reduced in lung cystic fibrosis airways .…”

Section: Discussionmentioning

confidence: 99%

Retracted: Circular RNA ANKRD36 attends to lipopolysaccharide‐aroused MRC‐5 cell injury via regulating microRNA‐31‐3p

2019

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Background Some circular RNAs (circRNAs) are reported to attend to the pathogenesis of pneumonia. This study tested the impact of circRNA ankyrin repeat domain 36 (circANKRD36) on human embryonic lung fibroblast MRC‐5 cell injury irritated by lipopolysaccharide (LPS). Methods After LPS irritation, viability, apoptosis, ROS, protein, and cytokines, along with circANKRD36 were tested by CCK‐8, Annexin V‐FITC, DCFH‐DA, and ELISA or Western blot. si‐circANKRD36 and microRNA‐31‐3p/5p (miR‐31‐3p/5p) inhibitor were applied to silence circANKRD36 and miR‐31‐3p/5p. miR‐31‐3p/5p mimic was utilized to upregulate miR‐31‐3p/5p. RT‐qPCR was used to detect miRNAs. The relationship between miRNAs and MyD88 or IL‐34 was analyzed by luciferase activity reporter assay. Results LPS aroused a decrease in viability, increases in apoptosis, ROS, and IL‐6, IL‐8, and TNF‐α, along with circANKRD36, and activation of NF‐κB pathway. Silencing circANKRD36 weakened the above‐mentioned influences of LPS. Moreover, silencing circANKRD36 hoisted miR‐31‐3p expression. Silencing miR‐31‐3p mitigated the impacts of circANKRD36 silence on LPS‐irritated MRC‐5 cells. Besides, MyD88 was a downstream target of miR‐31‐3p, and 3′UTR of IL‐34 mRNA was targeted by miR‐31‐5p. LPS induced the accumulation of MyD88. Silencing MyD88 was constructive to maintain cell viability, retard apoptosis and inhibit adverse oxidation and inflammation. Conclusion This research verified that silencing circANKRD36 could weaken LPS‐irritated MRC‐5 cell injury via regulating miR‐31/MyD88‐mediated repression of NF‐κB pathway.

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Section: Discussionmentioning

confidence: 99%

Retracted: Circular RNA ANKRD36 attends to lipopolysaccharide‐aroused MRC‐5 cell injury via regulating microRNA‐31‐3p

2019

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“…Thus, overexpressed miR-31 leads to keratinocyte differentiation, in part, via inhibiting the expression of FIH-1 proteins in human epidermal keratinocytes (HEKs) and human corneal epithelial keratinocytes (HCEKs) (18). In addition, Borska et al (19) identified a significant negative relationship between Endothelin-1 (ET-1) and miR-31 in psoriasis patients. According to their results, miR-31 may modulate apoptosis of psoriatic keratinocytes via inhibiting ET-1, which is produced by psoriatic keratinocytes to suppress keratinocyte apoptosis.…”

Section: Upregulated Mirnas In Psoriatic Keratinocytes Mir-31mentioning

confidence: 99%

miRNAs Flowing Up and Down: The Concerto of Psoriasis

Yang

Wang

2021

Front. Med.

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Psoriasis is a chronic immune-mediated skin disease, whose hallmarks include keratinocyte hyperproliferation and CD4+ T cell subsets imbalance. Dysregulated microRNAs (miRNAs) identified in psoriasis have been shown to affect keratinocyte and T cell functions, with studies on the molecular mechanisms and intrinsic relationships of the miRNAs on the way. Here, we focus on the dysregulated miRNAs that contribute to the two hallmarks of psoriasis with the miRNA target genes confirmed. We review a network, in which, upregulated miR-31/miR-203/miR-155/miR-21 and downregulated miR-99a/miR-125b facilitate the excessive proliferation and abnormal differentiation of psoriatic keratinocytes; upregulated miR-210 and downregulated miR-138 work in concert to distort CD4+ T cell subsets balance in psoriasis. The miRNAs exert their functions through regulating key psoriasis-associated transcription factors including NF-κB and STAT3. Whether flowing up or down, these miRNAs collaborate to promote the development and maintenance of psoriasis.

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“…The data show that, in addition to the hyperproliferation of keratinocytes, deceleration in keratinocyte apoptosis is also a significant pathological change observed in psoriasis [6–8]. Keratinocytes from the lesion of psoriasis are resistant to induction of apoptosis compared to keratinocytes from normal skin [9]. However, the current data on the apoptosis of psoriatic keratinocytes is limited.…”

Section: Introductionmentioning

confidence: 99%

Association Study of the Caspase Gene Family and Psoriasis Vulgaris Susceptibility in Northeastern China

Yao

Hao

Pei

2019

BioMed Research International

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Background. Abnormal apoptosis of keratinocytes is one of the pathological changes of psoriasis. Caspases (CASPs) are the central engines of apoptosis. Studies to date have shown that some SNPs alter the expression of related genes and lead to changes in disease risk. However, no studies have investigated the associations between gene polymorphisms and the risk of psoriasis in Han population in northeast China. Therefore, we conducted a case-control study to explore this question in Han population of northeastern China. Methods. 540 patients with PsV and 612 healthy age- and sex-matched controls were enrolled in this study. We determined the genotypes of 17 single nucleotide polymorphisms (SNPs) from 11 genes of caspase family by the improved multiplex ligation detection reaction (iMLDR) method. A model-based single SNP frequentist test and haplotype association studies were performed to evaluate the association between SNPs and PsV. Results. In the single SNP tests, rs6704688 in CASP8 was significantly associated with psoriasis vulgaris (PsV) in Han population of northeastern China (P = 0.0169, P’ = 0.0179 under the additive model; P = 0.0126, P’ = 0.0149 under the heterozygous model). In haplotype analyses, the CASP7 haplotype GC was found to be associated with PsV risk (case group versus control group, 47.2% versus 54.4%, respectively, p = 0.0149). Conclusions. Our study presented that the gene polymorphisms of CASP7 and CASP8 were significantly associated with PsV in Han population of northeastern China, which implied the functional relationship between PsV and caspase genes. CASP8 and CASP7 SNPs could be new potential biomarkers for risk stratification and prevention of PsV.

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Roles of miR-31 and Endothelin-1 in Psoriasis Vulgaris: Pathophysiological Functions and Potential Biomarkers

Cited by 21 publications

References 32 publications

Retracted: Circular RNA ANKRD36 attends to lipopolysaccharide‐aroused MRC‐5 cell injury via regulating microRNA‐31‐3p

Retracted: Circular RNA ANKRD36 attends to lipopolysaccharide‐aroused MRC‐5 cell injury via regulating microRNA‐31‐3p

miRNAs Flowing Up and Down: The Concerto of Psoriasis

Association Study of the Caspase Gene Family and Psoriasis Vulgaris Susceptibility in Northeastern China

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