Association Study of the Caspase Gene Family and Psoriasis Vulgaris Susceptibility in Northeastern China

Abstract

Background. Abnormal apoptosis of keratinocytes is one of the pathological changes of psoriasis. Caspases (CASPs) are the central engines of apoptosis. Studies to date have shown that some SNPs alter the expression of related genes and lead to changes in disease risk. However, no studies have investigated the associations between gene polymorphisms and the risk of psoriasis in Han population in northeast China. Therefore, we conducted a case-control study to explore this question in Han population of northeastern China. Methods. 540 patients with PsV and 612 healthy age- and sex-matched controls were enrolled in this study. We determined the genotypes of 17 single nucleotide polymorphisms (SNPs) from 11 genes of caspase family by the improved multiplex ligation detection reaction (iMLDR) method. A model-based single SNP frequentist test and haplotype association studies were performed to evaluate the association between SNPs and PsV. Results. In the single SNP tests, rs6704688 in CASP8 was significantly associated with psoriasis vulgaris (PsV) in Han population of northeastern China (P = 0.0169, P’ = 0.0179 under the additive model; P = 0.0126, P’ = 0.0149 under the heterozygous model). In haplotype analyses, the CASP7 haplotype GC was found to be associated with PsV risk (case group versus control group, 47.2% versus 54.4%, respectively, p = 0.0149). Conclusions. Our study presented that the gene polymorphisms of CASP7 and CASP8 were significantly associated with PsV in Han population of northeastern China, which implied the functional relationship between PsV and caspase genes. CASP8 and CASP7 SNPs could be new potential biomarkers for risk stratification and prevention of PsV.

Protective Action of SIRT1 Activator Aptamer in Human Skin Cell Line

“…Caspases are activated in a specific order during apoptosis (30). The results revealed that SNP at a high concentration (3mM) promotes cell apoptosis, consistent with earlier studies (23,31,32). Figure 4 shown gradual decrease in SYTO 16 fluorescent in dose dependent manner, when SIRT1 aptamer at dose (10 µM) green fluorescent almost disappeared and shown maximum fluorescent at dose (1.25 µM) that may explained as increased in SYTO16 fluorescence during apoptosis is caspase-dependent and the decrease of SYTO16 fluorescence was attributed by pharmacological inhibition of Caspases (33).…”

The Effect of Caspase 8, 9 Gene Polymorphisms on Non-Hodgkin Lymphoma Susceptibility and Clinical/Pathological Features

“…Their data showed that CC genotype (OR: 1.667, 95% CI: 0.967-2.876) and CT genotype (OR:1.435, 95% CI: 0.998-2.063) of rs4233532 was correlated with significantly increase risk of colorectal cancer (Liu et al, 2013). Moreover, Yao et al observed that heterozygous statistical model (T/C) of rs4233532 was associated with psoriasis susceptibility (Yao et al, 2019). However, we did not detect significant association between CASP9-rs4233532 polymorphism and NHL risk, in consistent to previous studies, we observed that C/T carriers of rs4333532 SNP had marginally significant association with risk of NHL.…”

Research Progress of Genomic Variation in Psoriasis

“…30 Yao et al . 31 found that SNP rs6704688 in the CASP8 family was significantly correlated with psoriasis vulgaris in the Northeast Han population of China. Chang et al .…”

“…29 The Affiliated Hospital of Guangdong Medical College team found that SNP rs3804099 located in TLR2 was significantly associated with psoriasis vulgaris in the population of southern China. 30 Yao et al 31 found that SNP rs6704688 in the CASP8 family was significantly correlated with psoriasis vulgaris in the Northeast Han population of China. Chang et al 32 found that the HCR gene, SNP n.7*A, SNP n.9*C, and Cw*0602 were the major susceptibility markers for patients with psoriasis in Taiwan, China.…”