Roles of matrix metalloproteinases in development, immunology, and ovulation in fruit Fly (<i>Drosophila</i>)

Bindhani, Banani; Maity, Sulagna; Chakrabarti, Ipsit; Saha, Samir K.

doi:10.1002/arch.21849

Cited by 5 publications

(5 citation statements)

References 52 publications

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“…The importance of MMPs in protein degradation can be evidenced by their wide distribution in different groups of vertebrates [44], including mammals [6][7][8][9], birds [45], reptiles [46,47], amphibians [48] and fish [10], as well as insects [49] and echinoderms [50].…”

Section: Discussionmentioning

confidence: 99%

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

de Oliveira,

Kotowski,

Sampaio-Filho

et al. 2023

Biomedicines

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In dentistry, various animal models are used to evaluate adhesive systems, dental caries and periodontal diseases. Metalloproteinases (MMPs) are enzymes that degrade collagen in the dentin matrix and are categorized in over 20 different classes. Collagenases and gelatinases are intrinsic constituents of the human dentin organic matrix fibrillar network and are the most abundant MMPs in this tissue. Understanding such enzymes’ action on dentin is important in the development of approaches that could reduce dentin degradation and provide restorative procedures with extended longevity. This in silico study is based on dentistry’s most used animal models and intends to search for the most suitable, evolutionarily close to Homo sapiens. We were able to retrieve 176,077 mammalian MMP sequences from the UniProt database. These sequences were manually curated through a three-step process. After such, the remaining 3178 sequences were aligned in a multifasta file and phylogenetically reconstructed using the maximum likelihood method. Our study inferred that the animal models most evolutionarily related to Homo sapiens were Orcytolagus cuniculus (MMP-1 and MMP-8), Canis lupus (MMP-13), Rattus norvegicus (MMP-2) and Orcytolagus cuniculus (MMP-9). Further research will be needed for the biological validation of our findings.

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Section: Discussionmentioning

confidence: 99%

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

de Oliveira,

Kotowski,

Sampaio-Filho

et al. 2023

Biomedicines

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“…Further, in the last few decades it has been reported that metastatic behavior of Drosophila tumors depends on Matrix metalloproteinases (MMPs) to support the microenvironment of cancer cells [5]. Matrix metalloproteinases (MMPs), one of the metastasis biomarker, are extracellular calcium-dependent zinc-containing proteases, which regulate many signaling pathways involved in cell differentiation, proliferation, and metastasis [6]. In humans there are 24 MMPs, of which, 7 MMPs are GPI (Glycosylphoshatidylinositol) anchored and 17 MMPs are soluble secreted proteinases [7].…”

Section: Introductionmentioning

confidence: 99%

Scribbleknockdown induced metastasis tracking, identification of its associated novel molecular candidates through Proteome andin silicostudies

Singh,

Srikrishna

2024

Preprint

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Metastasis is the primary cause of cancer associated death globally. Loss of function of Scribble, a cell polarity regulator/tumor suppressor gene, is associated with many forms of human cancer but its role in cell proliferation and metastasis remains unknown. We generated metastatic cancer in Drosophila using UAS-GAL4 system, through knockdown of Scribble in the wing imaginal discs and tracked metastasis events from 0hr early pupae to 84hrs late pupae using fluorescence microscope. Here, we report, for the first time, that knockdown of Scribble alone could lead to the development of primary tumor in the wing imaginal discs, which is capable of establishing metastasis, leading to secondary tumor formation, eventually resulting in absolute pupal lethality. Further, we checked a metastasis biomarker, MMP1 levels during pre-and post-metastatic phases in Drosophila pupae using qRT-PCR and Western blot analysis. In addition, we analyzed the proteome of Scribble knockdown induced tumor-bearing pupae by 2-D gel electrophoresis followed by MALDI-TOF MS to identify novel proteins involved in the process of tumorigenesis and metastasis. We identified six differentially expressed proteins, Obp 99b, Fer2LCH,CG13492, Hsp23, Ubiquitin and Colt in Scrib knockdown pupae compared to wild-type and validated their expression at the transcriptional level using qRT-PCR. In-silico studies show these novel protein interaction with Scrib. Thus, our results suggested that loss of Scrib alone causes metastasis, without the need for cooperative interaction with oncogenic Ras. The newly identified Fer2LCH (ferritin) and colt proteins could be important candidates for therapeutic target against Scrib associated cell proliferation and metastasis.

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“…MMPs are classified into 3 groups: MMP1, MMP2, and MMP3 in insects (Altincicek & Vilcinskas, 2008;Knorr et al, 2009;Kantor et al, 2017;Liu et al, 2022). In Drosophila, MMP1 is involved in trachea development and nerve development of embryos, fat body dissociation, while MMP2 mutations result in defective histolysis, but both MMP1 and MMP2 are required for tissue remodeling and wound healing (Bindhani et al, 2022). Furthermore, prior studies demonstrated that c-Jun N-terminal kinase stimulates MMP1 production, which is vital for metastasis in hindgut cells (Uhlirova & Bohmann, 2006;Weston & Davis, 2007;Bindhani et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, prior studies demonstrated that c‐Jun N‐terminal kinase stimulates MMP1 production, which is vital for metastasis in hindgut cells (Uhlirova & Bohmann, 2006; Weston & Davis, 2007; Bindhani et al. , 2022). In agreement with the findings in Drosophila , functional assays showed that the functions of MMPs are conserved in Bombyx mori , in which 3 MMP members have demonstrated their pleiotropic effects in immunity and metamorphosis (Liu et al.…”

Section: Introductionmentioning

confidence: 99%

“…In Drosophila, MMP1 is involved in trachea development and nerve development of embryos, fat body dissociation, while MMP2 mutations result in defective histolysis, but both MMP1 and MMP2 are required for tissue remodeling and wound healing (Bindhani et al, 2022). Furthermore, prior studies demonstrated that c-Jun N-terminal kinase stimulates MMP1 production, which is vital for metastasis in hindgut cells (Uhlirova & Bohmann, 2006;Weston & Davis, 2007;Bindhani et al, 2022). In agreement with the findings in Drosophila, functional assays showed that the functions of MMPs are conserved in Bombyx mori, in which 3 MMP members have demonstrated their pleiotropic effects in immunity and metamorphosis (Liu et al, 2022), as well as in Tribolium castaneum (Knorr et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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The selective regulation of immune responses by matrix metalloproteinase MMP14 in Ostrinia furnacalis

Chen

Song

et al. 2023

Insect Science

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Matrix metalloproteinases (MMPs) are crucial for tissue remodeling and immune responses in insects, yet it remains unclear how MMPs affect the various immune processes against pathogenic infections and whether the responses vary among insects. In this study, we used the lepidopteran pest Ostrinia furnacalis larvae to address these questions by examining the changes of immune‐related gene expression and antimicrobial activity after the knockdown of MMP14 and bacterial infections. We identified MMP14 in O. furnacalis using the rapid amplification of complementary DNA ends (RACE), and found that it was conserved and belonged to the MMP1 subfamily. Our functional investigations revealed that MMP14 is an infection‐responsive gene, and its knockdown reduces phenoloxidase (PO) activity and Cecropin expression, while the expressions of Lysozyme, Attacin, Gloverin, and Moricin are enhanced after MMP14 knockdown. Further PO and lysozyme activity determinations showed consistent results with gene expression of these immune‐related genes. Finally, the knockdown of MMP14 decreased larvae survival to bacterial infections. Taken together, our data indicate that MMP14 selectively regulates the immune responses, and is required to defend against bacterial infections in O. furnacalis larvae. Conserved MMPs may serve as a potential target for pest control using a combination of double‐stranded RNA and bacterial infection.

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Roles of matrix metalloproteinases in development, immunology, and ovulation in fruit Fly (Drosophila)

Cited by 5 publications

References 52 publications

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

Scribbleknockdown induced metastasis tracking, identification of its associated novel molecular candidates through Proteome andin silicostudies

The selective regulation of immune responses by matrix metalloproteinase MMP14 in Ostrinia furnacalis

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