Roles of matrix metalloproteinase-26 in the growth, invasion and angiogenesis of breast cancer

Abstract

Abstract. Matrix metalloproteinase-26 (MMP-26) is a novel member of the MMP family and plays a significant role in the progression of estrogen-dependent malignancies. The present study aimed to investigate the roles of MMP-26 in the growth, invasion and angiogenesis of breast cancer. pcDNA3.1(+)-neo expression plasmids carrying the proMMP-26 coding sequence were used to transfect a breast cancer cell line (MCF-7 cells). The mRNA and protein expression of MMP-26 was determined by RT-PCR, immunofluorescence analysis and flow cytometry. The morphology of transfected cells was observed under an electron microscope. An adherence and spreading assay, Boyden chamber assay, in vivo tumorigenicity assay and in vivo angiogenesis were further modeled to elucidate the roles of MMP-26 in the invasion and angiogenesis of breast cancer. Using electron microscopy, the MMP-26-transfected cells demonstrated increased atypia, including unusual mitotic figures, glucogen pools and special lysosomes in the cytoplasm. The adherence and spreading ability of MMP-26-transfected cells were increased significantly compared with cells in the control group. The Boyden chamber assay demonstrated that the migration and invasion ability of MMP-26-transfected cells was dramatically accelerated compared with the control group, but markedly reduced in the presence of anti-MMP-26 antibody. MMP-26 also increased the malignant phenotype in vivo. The number of vessel branches and the total length of vessels induced by MMP-26-transfected cells were significantly increased compared to those induced by non-transfected cells. The plasmid carrying the proMMP-26 gene was successfully transfected into breast cancer cells. Our results demonstrate that MMP-26 overexpression promotes the growth and invasion of breast cancer cells and induces angiogenesis.

Plasma Concentrations of Matrilysins MMP-7 and MMP-26 as Diagnostic Biomarkers in Breast Cancer

“…It is known that MMP-26 is involved in the development of oestrogen-dependent cancers, including breast cancer [ 42 , 43 ]. BC cells expressing MMP-26 are characterised by an increased number of mitotic figures, atypia, presence of glycogen fields and atypical lysosomes in the cytoplasm.…”

Plasma Matrilysins MMP-7 and MMP-26 Concentrations as Diagnostic Biomarkers for Breast Cancer Patients

“…The number and length of blood vessels produced as a result of induction by cells expressing MMP-26 was higher than those induced by tumour cells not expressing MMP-26. Expression of MMP-26 increased the malignant phenotype of these cells in vivo (20). However, literature lack data regarding plasma level of MMP-26 in BC patients.…”

“…It is known that MMP-26 is involved in the development of estrogen-dependent cancers, including breast cancer (19,20). BC cells expressing MMP-26 were characterized by an increase in the number of mitotic gures, atypia, the presence of glycogen elds and atypical lysosomes in the cytoplasm.…”

High MMP‑26 expression in glioma is correlated with poor clinical outcome of patients

“…However, no significant relationships with other clinicopathological parameters were identified. Previous studies have indicated the possible participation of MMP-26 in tumor invasion and metastasis ( 17 – 19 ). Hu et al identified that the expression of MMP-26 was correlated with cancer invasion and metastasis and served an important role in tumor progression in colorectal cancer ( 15 ).…”