Roles of focal adhesion proteins in skeleton and diseases

Chen, Sheng; He, Tailin; Zhong, Yiming; Chen, Mingjue; Yao, Qing; Chen, Di; Shao, Zengwu; Xiao, Guozhi

doi:10.1016/j.apsb.2022.09.020

Cited by 27 publications

(12 citation statements)

References 159 publications

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“…Another important pathway revealed by RNA-seq was the focal adhesions (FAs) pathway. Integrin mediated FAs activation with enhanced cell migration and motility 25 can orchestrate the interplay between stromal cells and immune cells. Finally, the oxidative phosphorylation and universal metabolic suppression in NXP2 + DM reflect the downstream effects of tissue necrosis.…”

Section: Discussionmentioning

confidence: 99%

Severe gastrointestinal involvements in patients with adult dermatomyositis with anti-NXP2 antibody

Fu,

Gu,

Chen

et al. 2024

RMD Open

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ObjectiveGastrointestinal (GI) involvements were scarcely reported in adult anti-nuclear matrix protein 2 (NXP2) dermatomyositis (NXP2+DM). In this study, we investigated the clinical, pathological and molecular features as well as treatment options of this rare yet life-threatening disease.MethodsWe retrospectively collected the data of the cohort of NXP2+DM from 2012 to 2022 in our hospital. RNA sequencing was performed in intestinal samples of perforated patients compared with healthy controls data set.ResultsA total of 56 patients with adult NXP2+DM were collected including 10 cases with GI involvements. Abdominal pain and melena were the initial manifestations for GI involvements with a median 10-month time lag after the diagnosis of NXP2+DM when myositis largely subsided. Within weeks, GI perforation occurred in 8 of 10 patients, while five patients underwent eight surgical interventions subsequently. The short-term mortality was observed in four patients. NXP2+DM with GI involvements presented with more extramuscular systemic manifestations such as interstitial lung disease and subcutaneous calcinosis. The GI pathological features encompassed vasculitis/vasculopathy with high MxA expression, intestinal smooth muscle necrosis and serosal calcinosis. Gene expression profile validated the type-I interferon activation and revealed that epithelial mesenchymal transition and focal adhesion pathway may also contribute. Finally, vedolizumab, an anti-α4β7-integrin monoclonal antibody, exhibited promising therapeutic signals which should be further investigated.ConclusionsGI involvement is a unique complication in patients with adult NXP2+DM. Timely recognition and targeted therapy may turn out to be lifesaving.

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Section: Discussionmentioning

confidence: 99%

Severe gastrointestinal involvements in patients with adult dermatomyositis with anti-NXP2 antibody

Fu,

Gu,

Chen

et al. 2024

RMD Open

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“…The molecular function integrin binding was an enriched GO term in both 50 nm and 500 nm PS exposure, while the canonical pathway integrin signaling was activated in 500 nm only. In astrocytes, integrin-mediated signaling is known to govern cell spreading, morphology, migration and proliferation, all hallmarks of astrogliosis [ 91 , 92 ].…”

Section: Discussionmentioning

confidence: 99%

Polystyrene Nano- and Microplastic Particles Induce an Inflammatory Gene Expression Profile in Rat Neural Stem Cell-Derived Astrocytes In Vitro

Marcellus,

Bugiel,

Nunnikhoven

et al. 2024

Nanomaterials

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Microplastics are considered an emerging environmental pollutant due to their ubiquitous presence in the environment. However, the potential impact of microplastics on human health warrants further research. Recent studies have reported neurobehavioral and neurotoxic effects in marine and rodent models; however, their impact on the underlying cellular physiology in mammals remains unclear. Herein, we exposed neural stem cells and neural stem cell-derived astrocytes, oligodendrocytes, and neurons to various sizes and concentrations of polystyrene nano- and microplastics. We investigated their cellular uptake, impact on cytotoxicity, and alteration of gene expression through transcriptome profiling. The cell type most affected by decreased viability were astrocytes after 7 days of repeated exposure. Transcriptional analysis showed that 1274 genes were differentially expressed in astrocytes exposed to 500 nm microplastics, but only 531 genes were altered in astrocytes exposed to 50 nm nanoplastics. Both canonical pathway and Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated pathways were involved in neuroinflammation, innate and adaptive immunity, cell migration, proliferation, extracellular matrix remodeling, and cytoskeleton structures. The downregulated pathways were involved in lipid metabolism, specifically fatty acid oxidation and cholesterol metabolism. Our results show that neural stem cell-derived astrocytes repeatedly exposed to nano- and microplastics for 7 days undergo changes that are hallmarks of astrogliosis.

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“…Taken together, it is likely that LIPUS may directly and indirectly inhibit osteoclasts through Ano1 and osteoblast PGE 2 production, respectively, that will be evaluated in future studies. In addition, there is great interest in whether the beneficial treatment effect of LIPUS in cartilage tissue was triggered upon focal adhesion kinase and kindlin-2 as the key cartilage integrity proteins [ 32 , 33 ]. The micro-CT analyses conveyed the result of the suppression of the catabolic activity, indicating that LIPUS acted as a preventative measure of high bone resorption in OA possibly suppressing the formation of bone marrow lesions.…”

Section: Discussionmentioning

confidence: 99%

Daily low-intensity pulsed ultrasound stimulation mitigates joint degradation and pain in a post-traumatic osteoarthritis rat model

Lee,

Georgas,

Komatsu

et al. 2024

Journal of Orthopaedic Translation

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Roles of focal adhesion proteins in skeleton and diseases

Cited by 27 publications

References 159 publications

Severe gastrointestinal involvements in patients with adult dermatomyositis with anti-NXP2 antibody

Severe gastrointestinal involvements in patients with adult dermatomyositis with anti-NXP2 antibody

Polystyrene Nano- and Microplastic Particles Induce an Inflammatory Gene Expression Profile in Rat Neural Stem Cell-Derived Astrocytes In Vitro

Daily low-intensity pulsed ultrasound stimulation mitigates joint degradation and pain in a post-traumatic osteoarthritis rat model

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