Roles of Chitosan in Green Synthesis of Metal Nanoparticles for Biomedical Applications

Phan, Thi Tuong Vy; Phan, Duc Tri; Cao, Xuan Thang; Huynh, Thanh‐Canh; Oh, Junghwan

doi:10.3390/nano11020273

Cited by 63 publications

(37 citation statements)

References 103 publications

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“…Furthermore, it was possible to observe that the antimicrobial activity of functionalized surfaces increased with the decrease of CS molecular weight, as demonstrated by biofilm cell composition and structure. In fact, results point to the CS2- and CS3-PLA as the most promising surfaces in reducing the number of biofilm cells, biovolume and biofilm thickness, possibly because low Mw chitosan penetrates the bacterial cell wall more easily due to their reduced size [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

et al. 2021

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Implantable medical devices (IMDs) are susceptible to microbial adhesion and biofilm formation, which lead to several clinical complications, including the occurrence of implant-associated infections. Polylactic acid (PLA) and its composites are currently used for the construction of IMDs. In addition, chitosan (CS) is a natural polymer that has been widely used in the medical field due to its antimicrobial and antibiofilm properties, which can be dependent on molecular weight (Mw). The present study aims to evaluate the performance of CS-based surfaces of different Mw to inhibit bacterial biofilm formation. For this purpose, CS-based surfaces were produced by dip-coating and the presence of CS and its derivatives onto PLA films, as well surface homogeneity were confirmed by contact angle measurements, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The antimicrobial activity of the functionalized surfaces was evaluated against single- and dual-species biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Chitosan-based surfaces were able to inhibit the development of single- and dual-species biofilms by reducing the number of total, viable, culturable, and viable but nonculturable cells up to 79%, 90%, 81%, and 96%, respectively, being their activity dependent on chitosan Mw. The effect of CS-based surfaces on the inhibition of biofilm formation was corroborated by biofilm structure analysis using confocal laser scanning microscopy (CLSM), which revealed a decrease in the biovolume and thickness of the biofilm formed on CS-based surfaces compared to PLA. Overall, these results support the potential of low Mw CS for coating polymeric devices such as IMDs where the two bacteria tested are common colonizers and reduce their biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

et al. 2021

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“…Over the last decade, other antimicrobial hybrid derivatives, such as nanocellulose/metal and nanocellulose/oxide metal, have attracted the attention of several researchers and industries due to their environmentally friendly status and low cost compared to synthetic polymer- (e.g., polylactic- co -glycolic acid (PLGA), polyvinylalcohol (PVA), poly(ethylene glycol) methyl ether- block -poly(lactide- co -glycolide) (mPEG-PLGA), chitosan, gelatin) encapsulated MNPs [ 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 ]. Cellulose is a ubiquitous natural polymer which can be produced from a broad range of biomass.…”

Section: General Background On Antimicrobial and Anti-biofilm Metalli...mentioning

confidence: 99%

A Critical Review of the Antimicrobial and Antibiofilm Activities of Green-Synthesized Plant-Based Metallic Nanoparticles

et al. 2022

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Metallic nanoparticles (MNPs) produced by green synthesis using plant extracts have attracted huge interest in the scientific community due to their excellent antibacterial, antifungal and antibiofilm activities. To evaluate these pharmacological properties, several methods or protocols have been successfully developed and implemented. Although these protocols were mostly inspired by the guidelines from national and international regulatory bodies, they suffer from a glaring absence of standardization of the experimental conditions. This situation leads to a lack of reproducibility and comparability of data from different study settings. To minimize these problems, guidelines for the antimicrobial and antibiofilm evaluation of MNPs should be developed by specialists in the field. Being aware of the immensity of the workload and the efforts required to achieve this, we set out to undertake a meticulous literature review of different experimental protocols and laboratory conditions used for the antimicrobial and antibiofilm evaluation of MNPs that could be used as a basis for future guidelines. This review also brings together all the discrepancies resulting from the different experimental designs and emphasizes their impact on the biological activities as well as their interpretation. Finally, the paper proposes a general overview that requires extensive experimental investigations to set the stage for the future development of effective antimicrobial MNPs using green synthesis.

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“…A variety of research groups have used them in imaging-guided PTT for in vivo cancer treatment 27 , 28 . Similarly, marine biopolymers such as chitosan (CS) have attracted considerable attention in recent years for their application in the cosmetics, nutraceutical, and pharmaceutical industries 29 , 30 . Manivasagan et al reported the combination of CS and PPy to be a strong NIR absorbance PTT agent for cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence/photoacoustic imaging-guided nanomaterials for highly efficient cancer theragnostic agent

Doan

Nguyen

et al. 2021

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Imaging modalities combined with a multimodal nanocomposite contrast agent hold great potential for significant contributions in the biomedical field. Among modern imaging techniques, photoacoustic (PA) and fluorescence (FL) imaging gained much attention due to their non-invasive feature and the mutually supportive characteristic in terms of spatial resolution, penetration depth, imaging sensitivity, and speed. In this present study, we synthesized IR783 conjugated chitosan–polypyrrole nanocomposites (IR-CS–PPy NCs) as a theragnostic agent used for FL/PA dual-modal imaging. A customized FL and photoacoustic imaging system was constructed to perform required imaging experiments and create high-contrast images. The proposed nanocomposites were confirmed to have great biosafety, essentially a near-infrared (NIR) absorbance property with enhanced photostability. The in vitro photothermal results indicate the high-efficiency MDA-MB-231 breast cancer cell ablation ability of IR-CS–PPy NCs under 808 nm NIR laser irradiation. The in vivo PTT study revealed the complete destruction of the tumor tissues with IR-CS–PPy NCs without further recurrence. The in vitro and in vivo results suggest that the demonstrated nanocomposites, together with the proposed imaging systems could be an effective theragnostic agent for imaging-guided cancer treatment.

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Roles of Chitosan in Green Synthesis of Metal Nanoparticles for Biomedical Applications

Cited by 63 publications

References 103 publications

Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

A Critical Review of the Antimicrobial and Antibiofilm Activities of Green-Synthesized Plant-Based Metallic Nanoparticles

Fluorescence/photoacoustic imaging-guided nanomaterials for highly efficient cancer theragnostic agent

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