Role or Synergistic Interaction of Adenosine and Vitamin D3 Alongside High-Intensity Interval Training and Isocaloric Moderate Intensity Training on Metabolic Parameters: Protocol for an Experimental Study

Abstract

Background Obesity is known as one of the major causes of epidemiologic diseases worldwide; therefore, the introduction of treatment strategies by medical professionals, such as the use of various medicines and exercise programs to reduce fat or prevent obesity, is on the rise. Recently, researchers have shown special interest in assessing the effect of lipolytic adenosine and vitamin D deficiency, as well as the effect of exercise, on decreasing body fat percentage. Objective This study has been designed to examine the effect of adenosine and vitamin D3 injections, in conjunction with high-intensity interval training and isocaloric moderate-intensity training, on the metabolic parameters of obesity induced by a high-fat diet. Methods This is an experimental study using 92 Wistar rats. At 6 weeks of age, the rats' weights will be recorded, after which they will have 1 week to adapt to their new environment before being divided into 12 groups. The rats will participate in a 2-stage experimental intervention, including a 13-week fattening diet phase followed by a 12-week exercise training phase consisting of an exercise program and the injection of adenosine and vitamin D3. Groups 1 and 2 will have a normal diet, and the other groups will have a diet of 40% fat, with free access to food and water up to the second half of the second stage of the study (end of the sixth week of training). After termination of the interventions, tissue collection and molecular assessments (blood for biochemical, tissues for gene expression analyses, and anthropometrical indexes) will be performed. Results The project was initiated in April 2017 and completed in December 2017. Data analysis is under way, and the first results are expected to be submitted for publication in November 2018. Conclusions We hypothesize that weight loss–induced molecular changes and upregulation will be observed in line with an increase in lipolysis and beta oxidation in muscle and fat tissue as a result of performing isocaloric training in drug-receiving rats and groups on a high-fat diet. International Registered Report Identifier (IRRID) RR1-10.2196/10753

Investigating Effects of Vitamin D Injection during a Course of Endurance Training On Anthropometrical Parameters of Wistar Rats with High-Fat Diet-Induced Obesity

“…The characteristic of animals is presented in table 1. The components of the high-fat-diet included 40 % fat, (20% soya bean oil, 20% animal fat), 14.1% protein, 36.58% carbohydrate, 8.4% mitral, and 0.72% vitamin (18). Statistical tests including analysis of variance repeated measure were employed to test the hypothesis of interest.…”

“…General characteristic of the rats has shown in table 1. The full details of the methodology of this study are presented in full (18). The exercise program was performed on a rodent treadmill.…”

High-intensity interval training and moderate intensity training with exogenous adenosine counteract development of obesity in rats

“…The EU Directive 2010/63/EU and the European Convention for Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Council of Europe No123, Strasbourg 1985) were followed. The complete study protocol has been previously published [18]. Seventy-seven male Wistar rats were purchased from the Shahid Mirghani Research institute (Golestan, Gorgan, Iran).…”

“…: intraperitoneal. The complete study protocol has been previously published [18]. minerals and 0.72% vitamins.…”