Role of Various Estrus Induction and Synchronization Protocols in Influencing Plasma Progesterone, Metabolic Profile and Fertility in Acyclic Buffaloes

Abstract

A study was carried out under field conditions on 50 acyclic/ anestrus buffaloes to evaluate the efficacy of four standard estrus synchronization protocols, viz., Doublesynch, Estradoublesynch, Ovsynch, and Ovsynch Plus (10 buffaloes in each protocol, and in untreated control group) in terms of estrus induction response, conception rates at induced estrus with FTAI and monitoring plasma progesterone, protein and cholesterol profile at different time intervals during treatment and day 12 post-AI. All the animals received pre-synchronization treatment, i.e., Inj. 100 mg ivermectin s/c, Inj. tono-vita 20 ml, and multi-minerals 1 bolus daily for 7 days. The conception rates obtained at induced estrus (FTAI) were 50.0, 40.0, 30.0 and 50.0 % with Doublesynch, Estradoublesynch, Ovsynch and Ovsynch Plus protocol, respectively. The rests were taken as non-conceived ones. The plasma progesterone concentrations monitored on day 0 (start of treatment), 7/9 (PGF2 α injection), 10/12 (FTAI) and on day 12 post-AI revealed significant (p Lass Than 0.01) effect of sampling days in all four protocols with higher values on day of PGF2 α injection and on day 12 post-AI compared to other days. Moreover, the plasma progesterone concentrations were significantly (p Lass Than 0.05) higher in conceiving than the non-conceiving buffaloes on day 12 post-AI in all 4 protocols. The mean plasma protein and cholesterol profile did not differ significantly between days in any of the protocols. The animals under Doublesynch protocol however had significantly higher protein values as compared to Ovsynch and Ovsynch Plus protocols. Moreover, the non-conceiving buffaloes under Ovsynch Plus protocol had significantly (p Lass Than 0.05) lower mean plasma protein (5.73±0.15 vs. 6.49±0.13 g/dl) and cholesterol (57.42±1.19 vs. 76.68±1.85 mg/dl) concentrations compared to their counterparts. It was concluded that all four hormonal protocols improved plasma progesterone profile and conception rates in acyclic buffaloes without altering plasma protein and cholesterol profile. The maximal benefit was with Doublesynch and Ovsynch plus protocols, hence these can be practiced under field conditions to manage acyclic buffaloes.

Changes in ovarian structures, blood biochemical and hormonal profile in double Ovsynch treated conceived and non-conceived Jaffarabadi buffaloes

“…In the present study, plasma progesterone levels, which were lowest during estrus, increased after 1 st GnRH and 3 rd GnRH injections on day 7 and day 24, respectively, and also increased levels were noticed on days 12, 21 and 35 post-AI in both conceived and non-conceived animals suggesting ovulatory estrus in both the subgroups, but perhaps with higher luteal insufficiency and/or early embryonic mortality in non-conceived group. These results are in agreement with earlier observations in buffaloes (Campanile et al 2007, Prajapati et al 2018.…”

Evaluation of Double Ovsynch Protocol in Acyclic Jaffarabadi Heifers and Buffaloes with Respect to Ovarian Dynamics, Hormonal Profile and Fertility

“…The mean values of plasma total protein increased with advancing age and differed significantly (p<0.001) at most of the periods, the values were lower in group I heifers and higher in postpartum buffaloes of group III (Table 7). These findings with respect to values coincided well with the previous reports of Singh et al (2004), Jerome et al (2016) and Prajapati et al (2018). However, other reserchers (Parmar et al, 2012;Chaudhary et al, 2018) reported higher values of total protein than the present findings.…”

“…The levels of plasma total cholesterol in animals under study varied from 57.40±5.44 to 96.60±7.55 mg/dl across different periods of treatment and groups without significant differences ( Table 7). The present value of plasma total cholesterol was in accordance with the report of Sharma et al (1998) and Prajapati et al (2018), but almost one half to those reported by Singh et al (2004), Parmar et al (2012) and Selvaraju et al (2017). Henricks et al (1971) were of the opinion that the highest adrenal cholesterol concentration occurs at estrus, when the females are under estrogen dominance eventually facing a decline later, when the progesterone phase sets in.…”