The extent and duration of serum lipid and lipoprotein changes were examined in 12 mildly obese women who walked 45 minutes at 60% VO2max in a laboratory setting. A two-factor, 2 x 6 design with repeated measures on both factors was utilized. The first factor was condition (exercise and rest) and the second factor was time (six times of measurement over a 24-hour period) with treatment counterbalanced. The patterns of change in total high-density lipoprotein-cholesterol (HDL-C) [F(5,55) = 5.75, p less than 0.001] and HDL3-C [F(5,55) = 2.62, p = 0.034], but not HDL2-C [F(5,55) = 1.15, p = 0.346], were significantly different between conditions. Relative to baseline and the rest condition, total HDL-C tended to rise due to a significant 11.6% increase in HDL3-C immediately post-exercise, with values returning to baseline 1.5 hours post-exercise. The interaction statistic for triglycerides was significant with trends for a decrease in triglycerides at 1.5 and 23 hours post-exercise relative to baseline and the rest condition. No significant differences were seen between the exercise and rest conditions for total cholesterol, low-density lipoprotein cholesterol, glucose, or plasma volume. These data indicate that an exercise intensity achievable by brisk walking (7.4 kph) is sufficient to evoke significant but short-term changes in serum HDL3-C concentrations in women.

The relative efficacy of different treatment modalities was evaluated for induction of estrus in 32 post-pubertal true anestrus Jafarabadi buffalo heifers randomly divided into four equal groups. Animals of Group I received intravaginal CIDR and i/m injection of 1.0 mg estradiol valerate on day 0, i/m injection of 500 μg PGF2α on day 7 while removing CIDR, and 0.75 mg estradiol valerate on day 8. Fix timed insemination (FTAI) was performed at 48 and 72 h following PGF2α injection. Animals of Group II received standard Ovsynch protocol with FTAI. In Group III, Prajana HS 3 caps/ day for 3 days along with i/m injection of Vitamin AD3E (5 ml) and Tonophosphan (15 ml) on first day were administered. Group IV animals received no treatment and served as control. Among all inseminated heifers, in non-return cases pregnancy was confirmed per rectum 60 days postbreeding. The estrus induction response in Group I and II animals was 100 %, whereas in Group III and IV it was 37.5 % and 12.5%, respectively. In Group I, one animal conceived at induced estrus and another four at second service while in Group II, five animals conceived at second service giving overall conception rate (CR) of 62.5% in each group within 77 days of treatment. In Group III, three animals conceived by 70 days and in Group IV, only one animal (12.5%) showed estrus and conceived by 50 days of treatment with overall CR of 37.5% and 12.5%, respectively. Mean plasma progesterone concentration was significantly (p less than 0.05) higher on day 7 as compared to day 0, at estrus and on day 20 post-AI in all the animals. It was concluded that both CIDR and Ovsynch protocols are better than herbal heat inducer + supplements in inducing fertile estrus in anestrus buffalo heifers.

Jaffrabadi buffalo is one of the heaviest buffalo breeds of the world (800 kg) and is known for poor reproductive efficiency. There is a scarcity of normal physiological data regarding reproductive parameters of Jaffrabadi buffalo. The primary objective of this study was to elucidate ovarian follicular dynamics, hormonal and biochemical profiles in 10 post-pubertal heifers (n=5 each of age 42±2 and 48±2 months) and five >90 days postpartum lactating buffaloes. Ultrasound monitoring of ovaries of animals was done at weekly interval and all follicles with diameter less than 4 mm (small follicles), 4-8 mm (medium follicles) and greater than 8 mm (large follicles) as well as CLs identified were recorded. Blood samples were also collected soon after each USG from all animals for assay of hormonal and biochemical profiles. The findings revealed that the average number of small, medium and large follicles, total number of follicles per ovary as well as diameters of largest and subordinate follicles varied greatly among periods between three groups. The size of corpus luteum ranged from 13.7 to 21.2 mm, 13.7 to 23.9 mm and 15.3 to 22.7 mm, respectively, in Group-I, II and III. In the first week of study, significantly higher (p less than 0.05) average numbers of small follicles in Group-I and III, and medium follicles and total numbers of follicles in Group-III were observed as compared to other groups. The diameter of largest follicles and subordinate follicles were non-significantly different among all groups at all periods of the study. Plasma estrogen levels were significantly higher (p less than 0.05) in Group-I as compared to Group-III and Group-II on first and second week, however, it increased significantly (p less than 0.05) in Group-II and I on fourth week of study period. Plasma insulin concentrations were significantly (p less than 0.01) lower in buffaloes (Group-III) than in heifers (Group-I and II) throughout the study period, while plasma total cholesterol and total protein levels were higher in Group-III on 3rd to 5th weeks of study. Further, there were no significant differences in the plasma FSH, LH, progesterone and blood glucose levels in animals of three groups between periods at any of the week. The ultrasonographic and endocrine profile suggested establishment of cyclicity with silent ovulation. However, the behavioural estrus was still not manifested by these post-pubertal heifers and postpartum lactating buffaloes. These results are helpful to diagnose anovulatory conditions and/or true anestrus and initiate estrus synchronization protocols for early conception in Jaffrabadi buffaloes.

In the polytocus small animals there may be a complete or a partial failure of the uterus to start contracting. In partial failure the uterus may bring the first fetus to the pelvic inlet from where it is delivered by abdominal straining. No further fetuses were present and uterine contraction ceases. An idiopathic type of primary uterine inertia has been described, in which delivery starts normally and several members of the litter are delivered normally. There is no evidence of obstruction to birth through maternal or fetal cause. The uterus stops contracting and does not resume unless ecbolic therapy is given. It could be argued that this could be classified as a partial rather than a complete primary uterine inertia (Peter Jackson, 2004).Uterine inertia that is lack of normal physiologic uterine contraction during or after parturition, is a common condition encountered in bovine. However, it is comparatively less reported in canine and feline.

A successful management of dystocia due to Hydramnios coupled with Bull dog calf in a Jaffarabadi buffalo was reported.

Primary or secondary uterine inertia is due to lack of normal physiological uterine contraction during or after parturition. Secondary uterine inertia is commonly observed in bovine, however, it is comparatively less reported in canine. In certain breeds of dog such as the Scotch terrier or Dachshund secondary uterine inertia may occur spontaneously early in the second stage of parturition. Usually, one or two fetuses are expelled normally and then labor ceases even though more fetuses are present in the uterus (Roberts, 1982). The present report places on record the difficult whelping due to secondary uterine inertia and its radiographic diagnosis and successful surgical management in 2 bitches.

Reproductive aspect of Jenny is very similar to that of Mare being that they both belong to the same genus Equus. The average length of gestation in donkeys varies between 10½ to 14½ months (Kirby, 1989). Dystocia is defined as any birth that reduces neonatal viability, causes maternal injury or requires assistance (Purohit and Honnappagole, 2009). Dystocia is much more common in primipara than pluripara (Roberts, 1986). Dystocia in Jenny can be caused by abnormalities of either maternal or foetal origin. In view of above facts a case of dystocia in Jenny due to right lateral deviation of fetal head with flexed left shoulder and its delivery by mutation is placed on record.

Advanced pregnant Jaffarabadi buffaloes (n=40) of 2-4 parity selected on an organized farm were divided equally into control (routine farm feeding-RFF) and treatment/nutrients supplementation (RFF + bypass fat @ 150-200 g/h/d and ASMM @ 50 g/h/d) groups and were studied from 6 wks prepartum to 8 wks postpartum for plasma profile of steroid hormones and PGF2a metabolites on days -45, -30, -7, 0, +7, +15, +30, +45 and +60 peripartum as well as for puerperal events and postpartum fertility. Half of the buffaloes in both the groups also received parenteral microminerals (Inj. Stimvet 5 ml i/m) twice, 45 days before and on the day of calving. Again half of them were treated with ecbolic (Exapar) 2 boli bid for first 4 days postpartum. The mean plasma progesterone values were maximum (>4 ng/ml) on day 45 prepartum, which declined significantly (p less than 0.01) on day 7 prepartum reached to the basal levels (1 ng/ml) on the day of calving, remained basal till day 15, and thereafter showed a rising trend on days 30, 45 and 60 postpartum. The oestradiol-17b values were at its peak on the day of calving (p less tahn 0.01), showed a rapid fall by day 7 postpartum and remained low till recrudesce of follicular activity around day 45 and 60 postpartum. The levels of cortisol were significantly higher on the day of parturition as compared to values on day 7 pre- and post-partum. The plasma concentration of PGFM was low on day 45 prepartum, which increased gradually and significantly by almost 10-folds to reach peak values on the day of calving in both control and treatment groups and then declined gradually and significantly till day 45 postpartum. The rise was little more in nutrients supplemented group with higher mean values at most intervals peripartum than in control group. The periods for uterine involution, first postpartum estrus, and days open were significantly shorter with higher conception rate in nutrient supplemented group. It is concluded that the peripartum nutrient supplementation in Jaffarabadi buffaloes is beneficial and has positive effect on the postpartum fertility and plasma progesterone and PGFM profile.