Role of Type X Collagen on Experimental Mineralization of Eggshell Membranes

Arias, José L.; Nakamura, Osamu; Fernández, María Soledad; Wu, Jiann Jiu; Knigge, P.; Eyre, David R.; Caplan, Arnold I.

doi:10.3109/03008209709160211

Cited by 76 publications

(44 citation statements)

References 73 publications

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“…This correlated with increased expression of Col10a1, a marker of chondrocyte maturation [47], in transplanted mice compared to nontransplanted oim, (1.0 -0.2$2 -DCt vs. 0.2 -0.1$2 -DCt respectively, P < 0.01).…”

Section: Fig 5 Transplanted Oim Have Increased Expression Of Genes mentioning

confidence: 76%

Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta

Jones

Moschidou

Abdulrazzak

et al. 2014

Stem Cells and Development

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Osteogenesis imperfecta (OI) is a genetic bone pathology with prenatal onset, characterized by brittle bones in response to abnormal collagen composition. There is presently no cure for OI. We previously showed that human first trimester fetal blood mesenchymal stem cells (MSCs) transplanted into a murine OI model (oim mice) improved the phenotype. However, the clinical use of fetal MSC is constrained by their limited number and low availability. In contrast, human fetal early chorionic stem cells (e-CSC) can be used without ethical restrictions and isolated in high numbers from the placenta during ongoing pregnancy. Here, we show that intraperitoneal injection of e-CSC in oim neonates reduced fractures, increased bone ductility and bone volume (BV), increased the numbers of hypertrophic chondrocytes, and upregulated endogenous genes involved in endochondral and intramembranous ossification. Exogenous cells preferentially homed to long bone epiphyses, expressed osteoblast genes, and produced collagen COL1A2. Together, our data suggest that exogenous cells decrease bone brittleness and BV by directly differentiating to osteoblasts and indirectly stimulating host chondrogenesis and osteogenesis. In conclusion, the placenta is a practical source of stem cells for the treatment of OI.

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Section: Fig 5 Transplanted Oim Have Increased Expression Of Genes mentioning

confidence: 76%

Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta

Jones

Moschidou

Abdulrazzak

et al. 2014

Stem Cells and Development

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“…Two studies showed no association between type X collagen and focal calcification sites (36,37). Furthermore, previous findings revealed that the non-helical domain of type X collagen must be removed to facilitate cell-mediated mineralization of the egg shell membrane, suggesting that type X collagen functions as an inhibitor of mineralization (38). In contrast, based on the findings that type X collagen expression precedes mineral formation (39), and its capacity to bind Ca 2ϩ and MV (16,40), it was proposed that type X collagen might prepare the matrix for subsequent mineralization.…”

Section: Fig 6 Camentioning

confidence: 98%

The Roles of Annexins and Types II and X Collagen in Matrix Vesicle-mediated Mineralization of Growth Plate Cartilage

Kirsch

Harrison

Golub

et al. 2000

Journal of Biological Chemistry

191

187

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Annexins II, V, and VI are major components of matrix vesicles (MV), i.e. particles that have the critical role of initiating the mineralization process in skeletal tissues. Furthermore, types II and X collagen are associated with MV, and these interactions mediated by annexin V stimulate Ca 2؉ uptake and mineralization of MV. However, the exact roles of annexin II, V, and VI and the interaction between annexin V and types II and X collagen in MV function and initiation of mineralization are not well understood. Annexins are a family of proteins that has in common the ability to bind to acidic phospholipids in the presence of Ca 2ϩ (1, 2). The family consists of at least 12 members, and three of them, annexins II, V, and VI, are highly expressed in calcifying cartilage and bone (3, 4). Annexin II and V each contain four 70 -80 amino acids repeats with an annexin consensus sequence. Annexin VI contains eight such repeats. These four or eight repeats form the conserved core region, which is responsible for the Ca 2ϩ -dependent binding of the proteins to phospholipids. In contrast, the N-terminal regions of the annexins are highly variable and may contribute to the specific functions of the various annexins (1, 2).Annexins II, V, and VI are major components of matrix vesicles (MV), 1 which are particles that, after being released from the plasma membrane of hypertrophic chondrocytes or osteoblasts, have the critical role of initiating the mineralization process in cartilage and perhaps in bone (3, 5). Three independent lines of evidence indicate that annexin II, V, and VI exhibit distinct Ca 2ϩ ion channel properties. First, when inserted into artificial phosphatidylserine bilayers they form voltage-dependent Ca 2ϩ ion channels (6 -8). Second, the crystal structures of these annexins are largely ␣-helical with parallel barrels of ␣-helical domains forming a hydrophilic, charged pore through the center of the protein (6, 8, 9). Third, annexin II, V, and VI are able to mediate Ca 2ϩ influx into artificial liposomes (10;11). It was shown that annexin-mediated Ca 2ϩ influx into liposomes is rapid during the first 20 min and then reaches a plateau after 20 min (10, 11).The initial phase of MV-mediated mineralization is characterized by the uptake of mineral ions by these particles and the formation and growth of the first mineral phase inside the vesicles (5). Because MV are enclosed by a membrane, channel proteins are required to mediate the influx of mineral ions into these particles. Previous findings from our and other laboratories, showed that chymotrypsin treatment, which removes most of the annexins from MV, and zinc treatment, which inhibits annexin-mediated Ca 2ϩ influx into phosphatidylserine (PS)-enriched liposomes, diminished MV Ca 2ϩ uptake (12-15), suggesting that annexins II, V, and VI serve as ion channels in MV, enabling Ca 2ϩ loading of the vesicles during the initial phase of mineralization.Previous studies have revealed that collagen types II and X are associated with the outer surface of MV (16). We...

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“…Directly above the basal lamina of the thin layer lies the ISM, an approximately 20 m thick, acellular, calcium-rich membrane containing peptidoglycans and collagen types I, V, and X. 15,18 The thin layer's basal lamina contains extensive microvilli that penetrate into the ISM 12 and contain calcium transporters that transfer calcium stored in the ISM to the developing embryo via the extraembryonic tissue. 19 -21 …”

mentioning

confidence: 99%

The Chick Chorioallantoic Membrane as a Model Tissue for Surgical Retinal Research and Simulation

Leng

Miller

Bilbao

et al. 2004

Retina

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Purpose:We describe the use of chick chorioallantoic membrane (CAM) as a model system for the study of the precision and safety of vitreoretinal microsurgical instruments and techniques.Methods: The CAM was prepared for experimentation with and without its inner shell membrane (ISM) attached for in vivo and in vitro experiments that simulated medical and surgical interventions on the retina.Results: The CAM's ease of use, low cost, and anatomic structure make it a convenient model for surgical retinal and retinal vascular modeling.Conclusion: While CAM has been used extensively in the past for ocular angiogenesis studies, we describe the tissue as a useful tool for a variety of other applications, including (1) testing of novel surgical tools and techniques for cutting and coagulating retina and its vasculature, (2) testing vessel cannulation and injection techniques, (3) angiographic studies, and (4) endoscopic surgery.

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Role of Type X Collagen on Experimental Mineralization of Eggshell Membranes

Cited by 76 publications

References 73 publications

Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta

Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta

The Roles of Annexins and Types II and X Collagen in Matrix Vesicle-mediated Mineralization of Growth Plate Cartilage

The Chick Chorioallantoic Membrane as a Model Tissue for Surgical Retinal Research and Simulation

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