Role of the support surface on the loading and the activity of Pseudomonas fluorescens lipase used for biodiesel synthesis

The Immobilized Lipases in Biodiesel Production

“…On the most hydrophobic support tested, Accurel MP1001, no glycerol adsorption was observed (Séverac et al, 2011). It has been demonstrated (Salis et al, 2009) comparing the catalytic efficiencies (activity/loading) of eight lipases, that they show a different level of adaptation to the support. Immobilized Pseudomonas fluorescens lipase is the most active biocatalyst, followed by immobilized Pseudomonas cepacia lipase.…”

“…Other synthetic polymers used for lipases immobilization comprise: hydrophobic polystyrene macroporous resin (Li & Yan, 2010), electrospun polyacrylonitrile nanofibers www.intechopen.com with higher porosity and interconnectivity compared with other nanostructured carriers (Sakai et al, 2010), polymethacrylate (Salis et al, 2009), etc. The naturally occurring materials used as carriers for lipase immobilization include: activated carbon (MorenoParajàn & Giraldo, 2011;Naranjo et al, 2010) and carbon cloth (Naranjo et al, 2010), celite (Ji et al, 2010;Shah & Gupta, 2007); hydrotalcite (Yagiz et al, 2007;Zeng et al, 2009), zeolites (Yagiz et al, 2007), etc.…”

Production of Candida antarctica Lipase B Gene Open Reading Frame using Automated PCR Gene Assembly Protocol on Robotic Workcell and Expression in an Ethanologenic Yeast for use as Resin-Bound Biocatalyst in Biodiesel Production

“…Weaker interactions appear more likely to preserve the enzyme in its active conformation. 19 Optimization of lipases and development of strains to express large quantities of these biocatalysts are necessary for inexpensive production of biodiesel. The scripting of automated protocols and scheduling of PCR assembly steps on the robotic workcell developed in our laboratory have the potential to be used in an iterative fashion for production of any gene open reading frame (ORF) and are demonstrated in this work for the production of a lipase gene ORF.…”

“…In addition, purification of biodiesel can be accomplished on a resin to produce a high-quality product that satisfies ASTM D6751 specifications. 18,19 Although the cost of the enzymatic catalyst remains a hurdle compared with that of the less-expensive chemical catalysts, the use of recombinant DNA technology to produce large quantities of lipases and the use of immobilized lipases may lower the cost of biodiesel production, while reducing downstream processing problems. 2,20e22 Differences have been observed in the catalytic activity of a lipase enzyme immobilized on different supports having different functional groups on their surface, allowing either physical or chemical adsorption.…”

“…2,20e22 Differences have been observed in the catalytic activity of a lipase enzyme immobilized on different supports having different functional groups on their surface, allowing either physical or chemical adsorption. 2,19,23 Surface structure of the support can affect enzyme activity through direct effects (type, strength, and orientation of enzyme-support interactions) or indirect effects (substrate-product interaction with the support affecting substrate-product partitioning between medium and binding site). In one study, the lowest loading of enzyme on a support showed the highest catalytic efficiency (activity of loaded protein).…”