The Immobilized Lipases in Biodiesel Production

Stoytcheva, Margarita; Montero, Gisela; Toscano, Lydia; Gochev, Velizar; Valdez, Benjamín

doi:10.5772/25246

Cited by 28 publications

(31 citation statements)

References 74 publications

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“…The recombinant ROL used is a promising new biocatalyst for biodiesel production that showed a 44-fold higher specific activity compared to a commercially available lipase obtained directly from R. oryzae, and a higher specificity towards the p-nitrophenol ester of long chain length [10]. rROL was immobilized by adsorption, because among immobilization techniques, it is simple and allows retention of high catalytic activity for lipases [11]. Among different supports, octadecyl-Sepabeads were chosen because this support revealed a high stability of rROL in a previous study [12].…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Synthesis in a Solvent‐Free System by Recombinant Rhizopus oryzae Lipase. Study of the Catalytic Reaction Progress

Canet

Benaiges

Valero

2014

J Americ Oil Chem Soc

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The recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL) was used to synthesize biodiesel and monoacylglycerols simultaneously. The reaction was carried out in a solvent-free system with the enzyme immobilized on octadecyl-Sepabeads. Using response surface methodology, the methyl ester yield was optimized by means of the study of the effect of water, substrate molar ratio (methanol:olive oil) and methanol stepwise addition. It was concluded that in order to prevent enzyme inactivation by methanol, alcohol should be added slowly; otherwise a large amount of water would be present. Taking the best conditions, a 50.3 % yield was achieved in 3 h, which corresponds to 75.4 % of the acyl groups at the 1,3-position undergoing transesterification. It was also concluded that methyl esters result from the esterification of the free fatty acid hydrolyzed by the enzyme and also from a direct transesterification of oil. In addition, the fatty acid selectivity of rROL was found not to favor one fatty acid in olive oil over another.

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Section: Introductionmentioning

confidence: 99%

Biodiesel Synthesis in a Solvent‐Free System by Recombinant Rhizopus oryzae Lipase. Study of the Catalytic Reaction Progress

Canet

Benaiges

Valero

2014

J Americ Oil Chem Soc

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“…Based on previous reports [20][21][22]25], the proposed one-pot encapsulation technique was evaluated regarding enzyme content to obtain an optimum transesterification activity. [13,16,26]. Secondly, it is renewable since it is produced by fermentation and finally, the ethanol has lower flammability and toxicity than methanol.…”

Section: Synthesis and Evaluation Of Biosilicified Pseudomonas Fluorementioning

confidence: 99%

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Ferrero

Faba

Eimer

2020

Preprint

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Background: Nowadays, as an alternative to the production of fuels and chemicals from the fossil platform, renewable feedstocks are widely investigated. For biomass conversion, a new generation of catalysts with specific characteristics such as high activity and selectivity, easy recovery and reusability is necessary. The design of highly efficient and stable heterogeneous catalysts represents a challenge in this field, mainly to overcome current energy and environmental issues. The combination of enzymatic and heterogeneous inorganic catalysis generates an unprecedented platform that combines the advantages of both. Among the techniques for producing solid catalysts, enzymatic mineralization with an organic silicic precursor to obtain hybrid biocatalysts (biosilicification) is highlighted. This technique can provide exceptional stability to the biocatalyst in drastic conditions of use.Results: Then, under these criteria, this work presents the one-step synthesis of a solid enzymatic catalyst, denominated Low Ordered Biosilicified Enzyme (LOBE) due to their structural properties. Pseudomonas Fluorescens lipase forms aggregates that are contained in the heart of a silicon-covered micelle, providing active sites with the ability to process different raw materials (commercial sunflower and soybean oil, Jatropha excisa oil, waste frying oil, residual soybeans, and pork fat) to produce first and second generation biodiesel. Obtaining yields between 81 and 93% by weight depending on the used raw material.Conclusions: Therefore, refined, non-edible and residual oils (with high water and free fatty acid contents) can be transformed into biodiesel through LOBE catalysts with commercial ethanol as co-substrate.

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“…Initial essays were carried out employing the encapsulated lipases as biocatalysts, and sun ower oil and food-, pharmaceutical-, and cosmetic-grade ethanol (96 v/v % and available in most local grocery stores) as raw materials. The latter was chosen for three reasons: (1) the enzyme needs the water to be active (4% v/v H 2 O is adequate; the water acts as a lubricant allowing the enzyme active site to open and interact with the substrates) [22,25,36], (2) it is produced from renewable sources, and (3) ethanol has lower ammability and toxicity than methanol.…”

Section: Synthesis and Evaluation Of Biosilici Ed Pseudomonas Uorescementioning

confidence: 99%

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Ferrero

Faba

Eimer

2020

Preprint

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Background: Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, highly active, selective, and easy to recover and reuse catalysts for biomass conversions is urgently needed. The combination of enzymatic and inorganic heterogeneous catalysis generates an unprecedented platform that combines the advantages of both, the catalytic efficiency and selectivity of enzymes with the ordered structure, high porosity, mechanical, thermal and chemical resistance of mesoporous materials to obtain enzymatic heterogeneous catalysts. Enzymatic mineralization with an organic silicon precursor (biosilicification) is a promising and emerging approach for the generation of solid hybrid biocatalysts with exceptional stability under severe use conditions. Herein, we assessed the putative advantages of the biosilicification technology for developing an improved efficient and stable biocatalyst for sustainable biofuel production. Results: A series of solid enzymatic catalysts denominated LOBE (Low Ordered Biosilicified Enzyme) were synthesized from Pseudomonas fluorescens lipase and tetraethyl orthosilicate. The microscopic structure and physicochemical properties characterization revealed that the enzyme formed aggregates that were contained in the heart of silicon-covered micelles, providing active sites with the ability to process different raw materials (commercial sunflower and soybean oils, Jatropha excisa oil, waste frying oil, acid oil from soybean soapstock, and pork fat) to produce first and second generation biodiesel. Ester content ranged from 81 to 93% wt depending on the raw material used for biodiesel synthesis. Conclusions: A heterogeneous enzymatic biocatalyst, LOBE4, for efficient biodiesel production was successfully developed in a single step synthesis reaction using biosilicification technology. LOBE4 showed to be highly efficient in converting refined, non-edible and residual oils (with high water and free fatty acid contents) and ethanol into biodiesel. Thus, LOBE4 emerges as a promising tool to produce second-generation biofuels, with significant implications for establishing a circular economy and reducing the carbon footprint.

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The Immobilized Lipases in Biodiesel Production

Cited by 28 publications

References 74 publications

Biodiesel Synthesis in a Solvent‐Free System by Recombinant Rhizopus oryzae Lipase. Study of the Catalytic Reaction Progress

Biodiesel Synthesis in a Solvent‐Free System by Recombinant Rhizopus oryzae Lipase. Study of the Catalytic Reaction Progress

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

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