Role of the Oral Microflora in Health

Marsh, Philip

doi:10.1080/089106000750051800

Cited by 102 publications

(65 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These microorganisms usually play a role in protection against pathogenic microbial invasion and the maintenance of oral health 3,4) . However, once microorganisms begin to form biofilm on the oral surfaces, e.g., teeth, oral mucosa, as well as on the surface of dental materials, they are capable of initiating and promoting oral diseases such as dental caries and periodontal diseases.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of Ti-Ag alloy on artificial biofilm formation

et al. 2014

View full text Add to dashboard Cite

Titanium-silver (Ti-Ag) alloy has been improved for machinability and mechanical properties, but its anti-biofilm properties have not been elucidated yet. Thus, this study aimed to evaluate the effects of Ti-Ag alloy on biofilm formation and bacterial viability in comparison with pure Ti, pure Ag and silver-palladium (Ag-Pd) alloy. Biofilm formation on the metal plates was evaluated by growing Streptococcus mutans and Streptococcus sobrinus in the presence of metal plates. Bactericidal activity was evaluated using a film contact method. There were no significant differences in biofilm formation between pure Ti, pure Ag and Ag-Pd alloy, while biofilm amounts on Ti-20% Ag and Ti-25% Ag alloys were significantly lower (p<0.05). In addition, Ti-Ag alloys and pure Ti were not bactericidal, although pure Ag and Ag-Pd alloy killed bacteria. These results suggest that Ti-20% Ag and Ti-25% Ag alloys are suitable for dental material that suppresses biofilm formation without disturbing healthy oral microflora.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of Ti-Ag alloy on artificial biofilm formation

et al. 2014

View full text Add to dashboard Cite

show abstract

“…4 It is considered a biofilm-dependent disease, because the pathogenic microbiota, which colonize and proliferate in dental biofilms, are the most important factor, along with a high-carbohydrate diet, for the onset and progression of the disease. [5][6][7][8] Virulence factors, including the adhesin antigens I/II (Ag I/II), glucosyltransferases, and glucan-binding protein, improve the ability of S. mutans to adhere and accumulate in the dental biofilm.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of p1025-loaded bioadhesive liquid-crystalline system for the prevention of <em>Streptococcus mutans </em>biofilms

Calixto¹,

Duque²,

Aida³

et al. 2017

IJN

View full text Add to dashboard Cite

Formation of a dental biofilm by Streptococcus mutans can cause dental caries, and remains a costly health problem worldwide. Recently, there has been a growing interest in the use of peptidic drugs, such as peptide p1025, analogous to the fragments 1025–1044 of S. mutans cellular adhesin, responsible for the adhesion and formation of dental biofilm. However, peptides have physicochemical characteristics that may affect their biological action, limiting their clinical performance. Therefore, drug-delivery systems, such as a bioadhesive liquid-crystalline system (LCS), may be attractive strategies for peptide delivery. Potentiation of the action of LCS can be achieved with the use of bioadhesive polymers to prolong their residence on the teeth. In line with this, three formulations – polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, oleic acid, and Carbopol C974P in different combinations (F1C, F2C, and F3C) were developed to observe the influence of water in the LCS, with the aim of achieving in situ gelling in the oral environment. These formulations were assessed by polarized light microscopy, small-angle X-ray scattering, rheological analysis, and in vitro bioadhesion analysis. Then, p1025 and a control (chlorhexidine) were incorporated into the aqueous phase of the formulation (F + p1025 and F + chlorhexidine), to determine their antibiofilm effect and toxicity on epithelial cells. Polarized light microscopy and small-angle X-ray scattering showed that F1C and F2C were LCS, whereas F3C was a microemulsion. F1C and F2C showed pseudoplastic behavior and F3C Newtonian behavior. F1C showed the highest elastic and bioadhesive characteristics compared to other formulations. Antibiofilm effects were observed for F + p1025 when applied in the surface-bound salivary phase. The p1025-loaded nanostructured LCS presented limited cytotoxicity and effectively reduced S. mutans biofilm formation, and could be a promising p1025-delivery strategy to prevent the formation of S. mutans dental biofilm.

show abstract

“…2,5 The mouth is similar to other sites in the body in that it has a natural microflora with a characteristic composition that confers benefit (see later). 6 However, on occasions, this beneficial relationship can break down and disease can occur (for example, dental caries, periodontal diseases), while halitosis can also be a consequence.…”

Section: Introductionmentioning

confidence: 99%