Role of the nucleoside-metabolizing enzymes on pain responses in zebrafish larvae

Gusso, Darlan; Wiprich, Melissa Talita; Altenhofen, Stefani; Bonan, Carla Denise

doi:10.1016/j.ntt.2022.107109

Cited by 3 publications

(10 citation statements)

References 53 publications

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“…The nociceptive impact of a 0.05% AA solution on zebrafish larvae is unmistakable, evidenced by a reduction in the normal exploratory behaviour across all specimens. Typically, when introduced in novel conditions, larvae tend to fiercely investigate their surroundings until they acclimate, which is reflected in their locomotor activity [ 26 ]. However, immersion in AA alters this consistent behavioural response, strongly suggesting the induction of a painful stimulus and corroborating previous research [ 27 , 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…In situations of repeated stress, the activity of ectonucleotidases is often altered, consequently influencing AMP, ADP, and ATP levels and antinociceptive mechanics [ 21 , 59 , 62 ]. Many studies suggest that an increase in AMP hydrolysis, and consequent adenosine generation, is often essential to how AA modulates ectonucleotidase activities [ 26 , 60 , 63 , 64 ]. In situations of repeated stress, there is evidence of a decreased AMP hydrolysis, which influences adenosine formation and justifies hypernociception [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…In situations of repeated stress, there is evidence of a decreased AMP hydrolysis, which influences adenosine formation and justifies hypernociception [ 64 ]. Adenosine synthesis in adequate quantities mainly occurs when both ATP and ADP levels are decreased because these nucleotides inhibit CD73, the 5′-ectonucleotidase responsible for AMP conversion [ 26 , 64 , 65 ]. Still, AMP hydrolysis is a complex process that might involve more than one enzymatic agent; in CD73 knockout mice, AMP hydrolysis still occurs, and it is less evident in lower pH environments [ 61 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

“…The zebrafish ( Danio rerio ) larval model has been established as an excellent alternative for studying pain perception due to its proven similarity with mammals’ biochemical and physiological processes integrated into this species’ behavioural responses [ 6 , 23 , 24 , 25 ]. Moreover, the nociceptive pain responses were confirmed in zebrafish larvae through exposure to nociceptive agents, such as acetic acid (AA), resulting in behavioural and biochemical alterations [ 26 , 27 ]. Therefore, this study evaluated the analgesic properties of active monoterpenes, such as eugenol, menthol, carvacrol, and thymol, assessing biochemical and behavioural endpoints.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Rocha,

Félix,

Monteiro

et al. 2024

Pharmaceuticals

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In the last decade, a considerable number of studies have broadened our knowledge of the nociceptive mechanisms of pain, a global health problem in both humans and animals. The use of herbal compounds such as eugenol, menthol, thymol, and carvacrol as analgesic agents has accompanied the growing interest in this area, offering a possible solution for this complex problem. Here, we aimed to explore how these natural substances—at three different concentrations (2, 5 and 10 mg/L)—affect the pain responses in zebrafish (Danio rerio) larvae exposed to 0.05% acetic acid (AA) for 1 min. By analysing the activity of acetylcholinesterase (AChE), 5′-ectonucleotidase and NTPDases, as well as aversion and exploratory behaviours, it was observed that that although all substances were effective in counteracting the pain stimulus, the concentration range within which they do so might be very limited. Eugenol, despite its acknowledged properties in fish anaesthesia, failed to alleviate the pain stimulus at low concentrations. Contrastingly, menthol exhibited the most promising results at the lowest concentrations tested. Overall, it is concluded that menthol might be a good analgesic for this species, qualifying it as a substance of interest for prospective studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Rocha,

Félix,

Monteiro

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…One of the major advantages of using zebrafish is that they feel pain with an indication of diving, erratic movements, and rubbing the injured area just such as we do [ 6 ]. In addition, they also respond to external stimuli such as thermal, pH changes, chemical irritants, and mechanical stimuli [ 8 ]. The progression of neuropathic pain is produced by potential cellular oxidative stress [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Reversal of Neuralgia Effect of Beta Carotene in Streptozotocin-Associated Diabetic Neuropathic Pain in Female Zebrafish via Matrix Metalloprotease-13 Inhibition

et al. 2023

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Beta carotene is a natural anti-oxidant agent, and it inhibits the matrix metalloprotease (MMP) activity. Diabetic neuropathic pain (DNP) is produced by cellular oxidative stress. The role of the beta carotene effect in diabetic neuropathic pain is not explored yet. The present study is designed for the evaluation of the palm oil mill effluent-derived beta carotene (PBC) effect in DNP in zebrafish. The DNP was induced by the intraperitoneal administration of streptozotocin (STZ). Blood glucose levels of above 15 mM were considered to be diabetic conditions. The zebrafish were exposed to test compound PBC (25, 50, and 100 µM), pregabalin (PG: 10 μM), and an MMP-13 inhibitor (CL-82198; 10 μM) for 10 consecutive days from day 11. The neuralgic behavioral parameters, i.e., temperature test, acetic acid test, and fin clip test were assessed on day 0 and the 7th, 14th, and 21st days. On the 22nd day, the blood glucose and MMP-13 levels and brain thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and MMP-13 activity levels were estimated. The treatment of PBC ameliorated the DNP-associated behavioral and biochemical changes. The results are similar to those of PG and CL-82198 treatments. Hence, the PBC possesses a potentially ameliorative effect against DNP due to its potential anti-oxidant, anti-lipid peroxidation, and MMP-13 inhibitory actions.

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Hypoxia-Induced Cardiac Arrest Alters Central Nervous System Concentrations of the GLYT2 Glycine Transporter in Zebrafish (Danio rerio)

Auzenne

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Hypoxia as a stressor has physiological implications that have been a focal point for many physiological studies in recent years. In some studies, hypoxia had large effects on the organ tissue degeneration, which ultimately effects multiple ecological processes. These organ tissue studies played a part in the development of new fields like neurocardiology, a specialty that studied the relationship between the brain and the heart. This thesis focuses on how hypoxia-induced cardiac arrest alters the amounts of GLYT2, a glycine reuptake transporter, in the central nervous system of zebrafish, Danio rerio. At 7 days post-fertilization (dpf), zebrafish were exposed to acute, severe hypoxia until they lost equilibrium, and minutes later, subsequent cardiac arrest occurred. Zebrafish were then placed into recovery groups to measure the GLYT2 levels at multiple points in zebrafish recovery. Fish were then sacrificed, and their brains dissected. Using immunofluorescence, the outer left optic tectum of the zebrafish was imaged, and mean image pixel fluorescent intensity was taken. There were significant changes (one-way ANOVA) in the levels of GLYT2 compared to that of the control groups during the course of recovery. GLYT2 levels continued to rise through the 24-hour recovery mark but did not show significant difference after 3 hours of recovery. This suggest that GLYT2 levels increased rapidly in the first 3 hours of recovery and continued to increase through 24 hours at a slower rate. Changes in GLYT2 levels may affect motor and sensory information, movement, visualization, and audition in these zebrafish. Further research should be conducted to determine how long it takes for GLYT2 levels to return to baseline, as well as behavioral measurements through each recovery period as it relates to glycine function.

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Role of the nucleoside-metabolizing enzymes on pain responses in zebrafish larvae

Cited by 3 publications

References 53 publications

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Reversal of Neuralgia Effect of Beta Carotene in Streptozotocin-Associated Diabetic Neuropathic Pain in Female Zebrafish via Matrix Metalloprotease-13 Inhibition

Hypoxia-Induced Cardiac Arrest Alters Central Nervous System Concentrations of the GLYT2 Glycine Transporter in Zebrafish (Danio rerio)

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