2010
DOI: 10.1016/j.lfs.2009.11.015
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Role of the membrane in the formation of heme degradation products in red blood cells

Abstract: Aims-Red blood cells (RBCs) have an extensive antioxidant system designed to eliminate the formation of reactive oxygen species (ROS). Nevertheless, RBC oxidant stress has been demonstrated by the formation of a fluorescent heme degradation product (ex.321 nm, em 465 nm) both in vitro and in vivo. We investigated the possibility that the observed heme degradation results from ROS generated on the membrane surface that are relatively inaccessible to the cellular antioxidants.Main Methods-Membrane and cytosol we… Show more

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Cited by 66 publications
(57 citation statements)
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“…The oxidative stress of erythrocytes accompanies many pathological conditions, such as sickle cell and iron deficiency anemias and thalassemia [8,29,30], as well as aging [17,31], hypoxia [9] and obesity [32]. An intensive search has been going on for natural antioxidants to treat diseases induced by RONS.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The oxidative stress of erythrocytes accompanies many pathological conditions, such as sickle cell and iron deficiency anemias and thalassemia [8,29,30], as well as aging [17,31], hypoxia [9] and obesity [32]. An intensive search has been going on for natural antioxidants to treat diseases induced by RONS.…”
Section: Discussionmentioning
confidence: 99%
“…The damage of erythrocytes, for example by thermal injury-induced hemolysis, can also be accomplished by the activation of neutrophils [6]. On the other hand, in different pathological situations, erythrocytes are a potential source of RONS due to autoxidation of hemoglobin which leads to formation of superoxide anions and hydroperoxides [7][8][9]. Oxidative stress induces the loss of cell plasticity with impaired deformability, associated with changes in the cytoskeleton network and also suicidal death of erythrocytes, known as eryptosis [10][11][12], which leads to the elimination of erythrocytes and finally to anemia.…”
Section: Introductionmentioning
confidence: 99%
“…The hypoxiainduced p65 translocation was inhibited in mice overexpressing the H 2 O 2 inhibitor catalase (28) ( Figure 6C, lanes 2 and 4), indicating that H 2 O 2 played a critical role in the response. In mice expressing endothelial-specific heterozygous mutations on Ser 32 and Ser 36 of the negative regulator of NF-kBa (IkBa 1/2 ) that block NF-kB activation (30), hypoxia did not cause nuclear p65 translocation ( Figure 6C, lanes 7 and 8), affirming that NFkB was activated in hypoxia. Further, in FLECs derived from IkBa 1/2 mice, HIF1a stabilization in hypoxia was absent ( Figure 6D), and the hypoxia-induced increase of E-selectin expression was markedly blunted ( Figures 6E, 6F, E6A, and E6B).…”
Section: Erythrocytes Activate Transcription Factorsmentioning
confidence: 77%
“…A mechanistic understanding of the erythrocyte effect was developed through a consideration of the role of Hb binding to erythrocyte membrane protein, band 3 (32). Although band 3 has multiple erythrocyte functions, including bicarbonatechloride exchange and cytoskeletal stability (33), the N-terminal cytoplasmic region of band 3 is the site for the competitive binding of deoxy-Hb and several glycolytic enzymes (22,31,34).…”
Section: Discussionmentioning
confidence: 99%
“…The metaanalysis, which states that 1% increase of RDW is associated with the 14% mortality increase in patients with Diabetes Mellitus, is the leading motive behind the research of these parameter (8). The reason of the increase of DM can not be enlightened completely, however it can be associated with oxidative stress and rapid erythrocyte elimination (9). Any significant relation in respect of RDW between patients with GDM and healthy pregnant women has not been found in the study of Erdogan et al (10).…”
Section: Discussionmentioning
confidence: 99%