Role of the intraspecific competition in the regulation of Agrobacterium tumefaciens transconjugant population level in soil experiments

Richaume, Agnès

doi:10.1016/0378-1097(92)90796-q

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…In both experiments, the lysogens formed due to infections stabilized at much lower densities than those of the parent strains. Similar observations have been made in studies of transconjugants in soil [11,19], although the dynamics of a population of plasmid-recombinants may differ from those of a lysogenized population.…”

Section: Discussionsupporting

confidence: 72%

Survival of the temperate actinophage φC31 and Streptomyces lividans in soil and the effects of competition and selection on lysogens

Marsh¹

1993

FEMS Microbiology Ecology

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Lysogenic infections were demonstrated m microcosms of sterde soil Inoculated with Streptomyces hvtdans and the thC31 derwatwe, KC301, in free state or via lysogemzed hosts Intermittent SOil mixing caused liberation of KC301 due to lysis of germinating lysogemzed and uninfected spores The presence of lysogentzed hosts ensured that KC301 was maintained at a constant density The lysogen S hwdans TK24 (KC301) achieved a population density lower than that of tts non-lysogemzed counterpart. Thiostrepton in the sod did not select for the thlostrepton resistance gene of KC301 The long-term survwal tn sod of a temperate actmophage was demonstrated.

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Section: Discussionsupporting

confidence: 72%

Survival of the temperate actinophage φC31 and Streptomyces lividans in soil and the effects of competition and selection on lysogens

Marsh¹

1993

FEMS Microbiology Ecology

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“…Pre-colonising the soil with the parent strain prevented extensive growth of the second inoculated strain when no amendment was applied to the soil. Such an e¡ect has already been observed in irradiated soil and has been term the`pre-emptive e¡ect' [16,20,22]. Antibiotic-resistant and parent strains may share the same ecological niches in soil.…”

Section: Discussionmentioning

confidence: 92%

Effect of carbon source supply and its location on competition between inoculated and established bacterial strains in sterile soil microcosm

Duquenne

Chenu

Richard

et al. 1999

FEMS Microbiology Ecology

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The aim of this work was to study how the location of a carbon source and of bacterial cells in soil can enhance the growth of a bacterial inoculum. Studies were performed using irradiated soil and two pairs of strains of Bradyrhizobium japonicum and Pseudomonas fluorescens. For each species, an antibiotic‐resistant mutant was used as inoculant and introduced into a sterile soil pre‐colonised with its parent strain. The inocula and a glycerol amendment were introduced together or separately into soil using porous microgranules or by spraying a suspension onto the bulk soil. Competition was assessed by plate counts of both inoculant and established populations. Both B. japonicum and P. fluorescens parent strains established at a high density level (109 cells g−1 of dry soil) in the sterile soil. The effects of the amendment on the inoculant strain in soil were strongly dependent on the way the cells and the substrate were introduced into the soil. Joint introduction of cells and substrate into soil improved the growth of the inoculant strain, but the effect of the amendment was also shown to be dependent on the inoculant carrier. Location of glycerol and the inoculant strain in microgranules gave a competitive advantage to the inoculated bacterium for a part of the amendment. We suggest that diffusion of glycerol from microgranules to the soil limits the competitive advantage of such location effects.

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Genetic transfer of the mcd gene in soil

et al. 2003

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Aims: To investigate the role of horizontal gene transfer of mcd (methylcarbamate-degrading) gene in high genetic diversity of carbofuran-degrading bacteria. Methods and Results: The actuality of genetic transfer from degraders to an Agrobacterium tumefaciens strain was determined in liquid medium. The mcd gene was chosen for transfer experiments. Transconjugants were obtained irrespective of the type of the donor strain (Gram-positive or Gram-negative), size of the inoculum, or nature and concentration of the pesticide in the medium. Soil microcosms, inoculated with or without the donor and/or recipient strains were used. The size of the initial degrading population (treated or untreated soil) and the nature of the inoculated donor strains were considered. More transconjugants were isolated in the previously treated soil than in the untreated soil. Agrobacterium transconjugants were isolated even when the donor strain was not inoculated, probably as a result of gene transfer from indigenous degrading population to the recipient strain. Moreover, potential transconjugants belonging to the Pseudomonas genus were isolated. Conclusions:Our results seem to demonstrate that the mcd gene is transferable in soil among bacterial populations. Significance and Impacts of the Study: The transfer of the mcd gene is partly responsible for the high genetic diversity of micro-organisms able to catabolize carbofuran.

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Role of the intraspecific competition in the regulation of Agrobacterium tumefaciens transconjugant population level in soil experiments

Cited by 3 publications

References 25 publications

Survival of the temperate actinophage φC31 and Streptomyces lividans in soil and the effects of competition and selection on lysogens

Survival of the temperate actinophage φC31 and Streptomyces lividans in soil and the effects of competition and selection on lysogens

Effect of carbon source supply and its location on competition between inoculated and established bacterial strains in sterile soil microcosm

Genetic transfer of the mcd gene in soil

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