Role of TGF-β and IL-6 in dendritic cells, Treg and Th17 mediated immune response during experimental cerebral malaria

Keswani, Tarun; Sarkar, Samrat; Sengupta, Anirban; Bhattacharyya, Arindam

doi:10.1016/j.cyto.2016.08.034

Cited by 33 publications

(37 citation statements)

References 72 publications

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“…For example, IL-1 was proven to support the activation of immune response induction [13]. IL-6 and TGF-β participate in immune regulation of dendritic cells [14]. Thus, we detected the concentrations of IL-1β, TNF-α, IL-6, IL-12, and IFN-γ.…”

Section: Discussionmentioning

confidence: 91%

Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells

et al. 2018

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BackgroundLnc-DC is a specific group of long non-coding (Lnc) RNAs in dendritic cells (DCs). Its function has been previously studied, and includes roles in dendritic cell differentiation and the progression of some diseases. In this study, we observed the critical role of Lnc-DC in regulating the differentiation, growth, and apoptosis of dendritic cells.MethodsWe first isolated peripheral blood mononuclear cells to culture and induce into DCs, which were then co-cultured with hepatitis B virus (HBV)-secreting HepG2.2.15 cells for the detection of changes in Lnc-DC. The expression levels of TLR9, p-STAT3, and SOCS3 were tested with qPCR and western blot. MTT assays were used to analyze the cell proliferation, cell cycle, and apoptosis. We used ELISA to test the expression of TNF-α, IL-1β, IL-6, IL-12p40, and IFN-γ.ResultsCo-culture with HBV-secreting HepG2.2.15 cells increased the level of Lnc-DC and activated TLR9/STAT3 signaling. The HBV DNA level (IU/ml) was positively correlated with levels of Lnc-DC and TLR9, further demonstrating that Lnc-DC was associated with the immune response of HBV. Lnc-DC was shown to regulate TLR9/STAT3 signaling in dendritic cells. More interestingly, the regulation of Lnc-DC controlled the immune response by reducing the concentration of secreted TNF-α, IL-6, IL-12, and IFN-γ, as well as increasing the IL-1β concentration in dendritic cells.ConclusionLnc-DC is important in regulating the growth, apoptosis, and immune response of dendritic cells mediated by TLR9/STAT3 signaling, and was also activated by HBV. This study provides a previously unidentified mechanism underlying the immune response in dendritic cells.

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Section: Discussionmentioning

confidence: 91%

Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells

et al. 2018

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“…IL-17-producing T cells termed Th17 cells secrete a set of antimicrobial cytokines including IL-22 and IL-21 and also mediate host protection against parasites and other pathogens (Sonnenberg, Monticelli, Elloso, Fouser, & Artis, 2011;Zhao et al, 2016). They are involved in immunity to intracellular infections including Cryptosporidium, Plasmodium spp., and Trypanosoma cruzi (Keswani, Sarkar, Sengupta, & Bhattacharyya, 2016;Zhao et al, 2014). However, pathogenic roles of Th17 responses have also been reported in the context of some parasitic infections (Cai, Blase, Zhang, Eickhoff, & Hoft, 2016;Keswani et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

IL‐17 and IL‐22 elicited by a DNA vaccine encoding ROP13 associated with protection against Toxoplasma gondiiin BALB/c mice

Alizadeh

Ahmadpour

Daryani

et al. 2018

Journal Cellular Physiology

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Toxoplasma gondii, an intracellular parasitic protozoan, is capable of infecting man and all warm‐blooded animals. Cell‐mediated immunity is vital in mounting protective responses against T. gondii infection. Recent studies have shown that T‐helper (Th) 17 responses may play a key role in parasite control. In this current study, we constructed a DNA vaccine encoding T. gondii ROP13 in a pcDNA vector. Groups of BALB/c mice were immunized intramuscularly with pcROP13 or controls and challenged with the RH strain of T. gondii. The results showed that immunization with pcROP13 could elicit an antibody response against T. gondii. The expression of the canonical Th17 cytokines, interleukin (IL)‐17 and IL‐22, were significantly increased after immunization with pcROP13 compared with control groups ( p < 0.05). Furthermore, vaccination resulted in a significant decrease in parasite load ( p < 0.05). The induction of Th17 related cytokines, using a ROP13 DNA vaccine, against T. gondii should be considered as a potential vaccine approach for the control of toxoplasmosis.

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“…In examining evidence of Th responses other than Th1 and Tfh, previous murine studies identified small numbers of splenic Th17 cells in Plasmodium-infected mice, but no major role for IL-17A (26)(27)(28). A recent study from last year suggested that IL-6, a known driver of Th17 differentiation in other systems, also contributed to modest Th17 activity in murine models (29). Nevertheless, there remains little evidence that IL-17A secreted by Th17 cells plays any role in controlling the outcome of blood-stage Plasmodium infection.…”

Section: Recent Insights Into Cd4 + Th Cell Differentiation In Malariamentioning

confidence: 99%

Recent Insights into CD4+ Th Cell Differentiation in Malaria

Soon

Haque

2018

The Journal of Immunology

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CD4 Th cell differentiation is crucial for protecting against blood-stage parasites, the causative agents of malaria. It has been known for decades that more than one type of Th cell develops during this infection, with early models proposing a biphasic Th1/Th2 model of differentiation. Over the past decade, a large body of research, in particular, reports over the past 2-3 y, have revealed substantial complexity in the Th differentiation program during infection. In this article, we review how several studies employing mouse models of malaria, and recent human studies, have redefined the process of Th differentiation, with a particular focus on Th1 and T follicular helper (Tfh) cells. We review the molecular mechanisms that have been reported to modulate Th1/Tfh differentiation, and propose a model of Th1/Tfh differentiation that accommodates observations from all recent murine and human studies.

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Role of TGF-β and IL-6 in dendritic cells, Treg and Th17 mediated immune response during experimental cerebral malaria

Cited by 33 publications

References 72 publications

Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells

Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells

IL‐17 and IL‐22 elicited by a DNA vaccine encoding ROP13 associated with protection against Toxoplasma gondiiin BALB/c mice

Recent Insights into CD4+ Th Cell Differentiation in Malaria

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