IL‐17 and IL‐22 elicited by a DNA vaccine encoding ROP13 associated with protection againstToxoplasma gondiiin BALB/c mice

Alizadeh, Paria; Ahmadpour, Ehsan; Daryani, Ahmad; Kazemi, Tohid; Spotin, Adel; Mahami-Oskouei, Mahmoud; Flynn, Robin J.; Azadi, Yaghob; Rajabi, Saba; Sandoghchian, Siamak

doi:10.1002/jcp.27747

Cited by 15 publications

(7 citation statements)

References 39 publications

(83 reference statements)

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“…The ROP4 protein is associated with the vacuole membrane and becomes phosphorylated in the infected host. T. gondii invasion of the host cell involves the secretion of recently identified ROP13 protein, which interacts with the various host cytoplasmic compartments [9,19]. Since these two proteins have never been studied in combination, we generated ROP4 VLPs, ROP13 VLPs, ROP4+ROP13 VLPs, and ROP(4 + 13) VLPs to evaluate their efficacies against T. gondii ME49.…”

Section: Introductionmentioning

confidence: 99%

Influenza Virus-Like Particles Presenting both Toxoplasma gondii ROP4 and ROP13 Enhance Protection against T. gondii Infection

et al. 2019

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Rhoptry organelle proteins (ROPs) secreted by Toxoplasma gondii (T. gondii) play a critical role during parasite invasion into host cells. In this study, virus-like particles (VLPs) vaccines containing ROP4 and/or ROP13 together with influenza M1 were generated. ROP4+ROP13 VLPs were produced by combining ROP4 VLPs with ROP13 VLPs, and ROP(4 + 13) VLPs by co-infecting insect cells with recombinant baculovirus expressing ROP4 or ROP13. Mice intranasally immunized with ROP(4 + 13) VLPs showed significantly higher levels of IgG, IgG1, IgG2a and IgA antibody responses in sera compared to ROP4+ROP13VLPs. Upon challenge infection by oral route, mice immunized with ROP(4 + 13) VLPs elicited higher levels of IgG and IgA antibody responses in fecal, urine, intestine and vaginal samples as well as CD4+ T, CD8+ T cells, and germinal center B cell responses compared to other type of vaccines, ROP4 VLPs, ROP13 VLPs, and ROP4+ROP13 VLPs. ROP(4 + 13) VLPs vaccination showed a significant decrease in the size and number of cyst in the brain and less body weight loss compared to combination ROP4+ROP13 VLPs upon challenge infection with T. gondii ME49. These results indicated that the ROP(4 + 13) VLPs vaccination provided enhanced protection against T. gondii infection compared to ROP4+ROP13 VLPs, providing an important insight into vaccine design strategy for T. gondii VLPs vaccines.

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Section: Introductionmentioning

confidence: 99%

Influenza Virus-Like Particles Presenting both Toxoplasma gondii ROP4 and ROP13 Enhance Protection against T. gondii Infection

et al. 2019

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“…Indeed, IL-17 receptordeficient mice displayed increased mortality when infected with T. gondii, which has been attributed to impaired neutrophil recruitment to infected sites [49]. Moreover, the production of IL-17A has been associated with reduced parasitic burden in mice immunized with T. gondii protein ROP13 [50]. Here, IL-17A production was evident in parasite antigenstimulated leukocyte cells obtained from immunized mice prior or after infection.…”

Section: Discussionmentioning

confidence: 91%

Protective Effect against Neosporosis Induced by Intranasal Immunization with Neospora caninum Membrane Antigens Plus Carbomer-Based Adjuvant

et al. 2022

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Neospora caninum is an obligate intracellular protozoan responsible for abortion and stillbirths in cattle. We previously developed a mucosal vaccination approach using N. caninum membrane proteins and CpG adjuvant that conferred long-term protection against neosporosis in mice. Here, we have extended this approach by alternatively using the carbomer-based adjuvant Carbigen™ in the immunizing preparation. Immunized mice presented higher proportions and numbers of memory CD4+ and CD8+ T cells. Stimulation of spleen, lungs and liver leukocytes with parasite antigens induced a marked production of IFN-γ and IL-17A and, less markedly, IL-4. This balanced response was also evident in that both parasite-specific IgG1 and IgG2c were raised by immunization, together with specific intestinal IgA. Upon intraperitoneal infection with N. caninum, immunized mice presented lower parasitic burdens than sham-immunized controls. In the infected immunized mice, memory CD4+ T cells predominantly expressed T-bet and RORγt, and CD8+ T cells expressing T-bet were found increased. While spleen, lungs and liver leukocytes of both immunized and sham-immunized infected animals produced high amounts of IFN-γ, only the cells from immunized mice responded with high IL-17A production. Since in cattle both IFN-γ and IL-17A have been associated with protective mechanisms against N. caninum infection, the elicited cytokine profile obtained using CarbigenTM as adjuvant indicates that it could be worth exploring for bovine neosporosis vaccination.

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“…In another study, T. gondii nucleoside triphosphate hydrolase-II (NTPase-II) coated with lipid NPs showed an increased protective effect against T. gondii RH strain (1 × 10 3 ) infection, and a significantly prolonged survival time was observed compared to immunization with the NTPase-II vaccine alone ( Luo et al., 2017 ). Various studies have analyzed the presence of T. gondii in different tissues of vaccine-injected mice or non-vaccine immunization groups by qualitative PCR to evaluate the protective effect against T. gondii infection ( Lu et al., 2017 ; Alizadeh et al., 2019 ). We investigated the parasite load in the present study.…”

Section: Discussionmentioning

confidence: 99%

Enhancing Immune Responses to a DNA Vaccine Encoding Toxoplasma gondii GRA7 Using Calcium Phosphate Nanoparticles as an Adjuvant

Sun

Huang

et al. 2021

Front. Cell. Infect. Microbiol.

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Toxoplasma gondii infects almost all warm-blooded animals, including humans. DNA vaccines are an effective strategy against T. gondii infection, but these vaccines have often been poorly immunogenic due to the poor distribution of plasmids or degradation by lysosomes. It is necessary to evaluate the antigen delivery system for optimal vaccination strategy. Nanoparticles (NPs) have been shown to modulate and enhance the cellular humoral immune response. Here, we studied the immunological properties of calcium phosphate nanoparticles (CaPNs) as nanoadjuvants to enhance the protective effect of T. gondii dense granule protein (GRA7). BALB/c mice were injected three times and then challenged with T. gondii RH strain tachyzoites. Mice vaccinated with GRA7-pEGFP-C2+nano-adjuvant (CaPNs) showed a strong cellular immune response, as monitored by elevated levels of anti-T. gondii-specific immunoglobulin G (IgG), a higher IgG2a-to-IgG1 ratio, elevated interleukin (IL)-12 and interferon (IFN)-γ production, and low IL-4 levels. We found that a significantly higher level of splenocyte proliferation was induced by GRA7-pEGFP-C2+nano-adjuvant (CaPNs) immunization, and a significantly prolonged survival time and decreased parasite burden were observed in vaccine-immunized mice. These data indicated that CaPN-based immunization with T. gondii GRA7 is a promising approach to improve vaccination.

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IL‐17 and IL‐22 elicited by a DNA vaccine encoding ROP13 associated with protection againstToxoplasma gondiiin BALB/c mice

Cited by 15 publications

References 39 publications

Influenza Virus-Like Particles Presenting both Toxoplasma gondii ROP4 and ROP13 Enhance Protection against T. gondii Infection

Influenza Virus-Like Particles Presenting both Toxoplasma gondii ROP4 and ROP13 Enhance Protection against T. gondii Infection

Protective Effect against Neosporosis Induced by Intranasal Immunization with Neospora caninum Membrane Antigens Plus Carbomer-Based Adjuvant

Enhancing Immune Responses to a DNA Vaccine Encoding Toxoplasma gondii GRA7 Using Calcium Phosphate Nanoparticles as an Adjuvant

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