Role of temperate phage in determining lytic phage sensitivity and serotype of Vibrio cholerae

Abstract

The effect of lysogenization with five temperate phages from various sources on serotype and lytic phage sensitivity was investigated in six cultures of Vibrio cholerae of both classical and El Tor biotypes. No changes in serotype or in classical phage sensitivity in the classical biotype were observed. Four of the temperate phages were homoimmune and induced resistance to one of the El Tor typing phages, E3, thereby causing a type change in El Tor strains. The sensitivity to the other phages was not changed. In 14 natural isolates too, E3 (group III) phage resistance correlated with the presence of temperate phage. Postadsorption exclusion was found to be the mechanism of resistance involved. The fifth phage, VcA-1, had a unique immunity profile. It could infect the El Tor biotype of V. cholerae but caused no change in the host properties investigated.

Development of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli

“…In addition to variations in typing results caused by time or methodology, Campylobacter serotyping and phage typing systems have the potential for modification based on unanticipated antigenic changes. Phage conversion, plasmids, and lysogeny, for example, have caused well-documented instances of changes in serotypes or phage types for Campylobacter species (21) and other organisms (5,31). The impact of these phenomena on the performance and reproducibility of Campylobacter typing systems needs to be determined.…”

Conversion of Vibrio eltor MAK757 to Classical Biotype: Role of Phage PS166

“…Although the O139 strains were shown to be derived from a V. eltor serogroup O1 strain and progenitor strains were identified , nothing is known about the genesis of the V. eltor strain. Phage conversion leading to changes in the serotype of V. eltor O1 strains have been reported (Ogg et al, 1972;Siddiqui et al, 1982). In V. eltor strains, changes in the phage type and toxigenicity due to phage-mediated induction have been reported (Siddiqui and Bhattacharyya, 1982).…”

“…Serotyping, resistance to polymyxin B, and production of hemagglutinin Serotyping of different colonies was performed according to the procedure described by Siddiqui and Bhattacharya (1982). Resistance to polymyxin B was determined according to Han et al (1963).…”

Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru

“…Historically, a major emphasis was placed on phage typing of V. cholerae (Basu and Mukerjee, 1968;Chattopadhyay et al ., 1993) and phage therapy was proposed for treatment of cholera in the early years of cholera research. Subsequent studies showed that temperate bacteriophages play a role in the lytic sensitivity and serotype conversion of Vibrio cholerae (Siddiqui and Bhattacharyya, 1982;Mitra, 1989). Recently, there has been renewed interest in V. cholerae phages because of their role in lateral transfer of genes in the environment and emergence of toxigenic strains.…”