Role of TAF15b in transcriptional regulation of autonomous pathway for flowering

Eom, Hyunjoo; Lee, Ilha

doi:10.1080/15592324.2018.1471300

Cited by 4 publications

(2 citation statements)

References 23 publications

(24 reference statements)

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“…In the ower buds of the high-TPC materials, the TAF15b promoter was hypomethylated, leading to signi cantly higher gene expression compared to the low-TPC lines. TAF15b participates in the autonomous pathway of owers, inhibits the transcription of FLOWERING LOCUS C, and regulates the development of oral organs [58][59]. Floral organ development plays an important role in silique development, which in turn is important for determining the TPC trait.…”

Section: Discussionmentioning

confidence: 99%

MiRNA-mediated Changes in DNA Methylation Affect the Expression of Genes Involved in the Thickness of Pod Canopy Trait in Brassica Napus

Chen

Jia

Wan

et al. 2021

Preprint

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Background: Methylation plays an important role in regulating crop development, but little is known about how methylation regulates plant architecture in rapeseed (Brassica napus). Here, we examined how methylation affects the TPC (thickness of pod canopy) trait in rapeseed by performing genome-wide methylation analysis of two extreme TPC lines. Results: We detected significant differences in overall methylation levels between the high- and low-TPC lines in the CG, CHG, and CHH contexts in the promoters of genes in the stem apex and flower bud. In flower buds, 26 genes had significantly higher methylation levels in the high-TPC samples compared to the low-TPC samples, resulting in significantly reduced gene expression. By contrast, in the stem apex samples, the promoter regions of 22 genes were hypermethylated in the high- vs. low-TPC samples. The promoters of 19 and 21 genes had significantly reduced methylation levels in the flower bud and stem apex, respectively, of the high- vs. low-TPC samples, resulting in significantly higher expression levels. Some of these differentially expressed genes are associated with TPC-related traits, such as BnaC03g53050D (UBC32), BnaA05g26660D (CYSB), BnaA10g07880D (TCP 1), BnaAnng09670D (SMP1), BnaA09g02000D (SDH2-2), BnaC01g12960D (NRT1.8), and BnaC09g30490D (TAF15b). In addition, 14 important genes related to growth and development were differentially regulated between the two groups due to miRNA-mediated differences in methylation levels in their promoters. For example, hypermethylation in the promoter region of BnaCnng64040D (Lipase family protein) mediated by miR159a led to significantly reduced gene expression in flower buds of high-TPC vs. low-TPC lines. Conclusions: These results, together with our previously generated RNA-seq and miRNA profiling data, indicate that both methylation and miRNAs are involved in regulating the expression of genes in nitrogen-related metabolic pathways, thereby affecting the TPC trait in B. napus, providing a reference for uncovering the molecular mechanism regulating this crucial trait.

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Section: Discussionmentioning

confidence: 99%

MiRNA-mediated Changes in DNA Methylation Affect the Expression of Genes Involved in the Thickness of Pod Canopy Trait in Brassica Napus

Chen

Jia

Wan

et al. 2021

Preprint

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“…The proteins encoded by FMI genes boost floral development by promoting flower development genes as well as by repressing AGL24, a promoter of inflorescence fate [ 1 ]. The main pathways orchestrating blossoming mediated by the internal and external signals can be divided into several types: the floral inhibition pathway [ 2 ], the autonomous pathway [ 3 ], the photoperiod pathway [ 4 ], the vernalization pathway [ 5 ], the gibberellin (GA) pathway [ 6 ], the stress pathway [ 7 ] and the aging pathway [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?

Shi,

Wang,

Wang

et al. 2023

Horticulture Research

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Recognized as a pivotal developmental transition, flowering marks the continuation of a plant’s life cycle. Vernalization and photoperiod are two major flowering pathways orchestrating numerous florigenic signals. Methylation, including histone, DNA and RNA methylation, is one of the recent foci in plant development. Considerable studies reveal that methylation seems to show an increasing potential regulatory role in plant flowering via altering relevant gene expression without altering the genetic basis. However, little has been reviewed about whether and how methylation acts on vernalization- and photoperiod-induced flowering before and after FLOWERING LOCUS C (FLC) reactivation, what role RNA methylation plays in vernalization- and photoperiod-induced flowering, how methylation participates simultaneously in both vernalization- and photoperiod-induced flowering, the heritability of methylation memory under the vernalization/photoperiod pathway, and whether and how methylation replaces vernalization/photoinduction to regulate flowering. Our review provides insight about the crosstalk among the genetic control of the flowering gene network, methylation (methyltransferases/demethylases) and external signals (cold, light, sRNA and phytohormones) in vernalization and photoperiod pathways. The existing evidence that RNA methylation may play a potential regulatory role in vernalization- and photoperiod-induced flowering has been gathered and represented for the first time. This review speculates about and discusses the possibility of substituting methylation for vernalization and photoinduction to promote flowering. Current evidence is utilized to discuss the possibility of future methylation reagents becoming flowering regulators at the molecular level.

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Methylation in DNA, histone, and RNA during flowering under stress condition: A review

Shi

Wang

et al. 2022

Plant Science

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Role of TAF15b in transcriptional regulation of autonomous pathway for flowering

Cited by 4 publications

References 23 publications

MiRNA-mediated Changes in DNA Methylation Affect the Expression of Genes Involved in the Thickness of Pod Canopy Trait in Brassica Napus

MiRNA-mediated Changes in DNA Methylation Affect the Expression of Genes Involved in the Thickness of Pod Canopy Trait in Brassica Napus

Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?

Methylation in DNA, histone, and RNA during flowering under stress condition: A review

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