Role of T. vulgaris protects DNA damage induced by Lead acetate in Rats

Abstract

Objective: The purpose of this study was to investigate Thymus vulgaris (T.vulgaris) ethanolic extract protective role for lymphocytes viability and prevent DNA damage by long-term exposure to Lead acetate (Pb) via ingestion.
 Material and method: A number of 24 females adult rats were divided into equal groups; 1st Group, as control, 2nd Group administered with lead acetate 5 mg/kg B.W oral gavage lead acetate for 60 days and 3rd Group treated with lead acetate at dose 5mg/kg B.W and T.vulgaris extract at doses 75mg/kg B.W for 60 days.
 Result: Results of leukocytes (WBCs) and lymphocyte count revealed a significant (P≤0.05) decrease in the 2nd group and an increase in the 3rd group in comparison to the 1st group. Also the result of Lymphocyte/Neutrophil ratio increased significantly (P≤0.05) in the 2nd and 3rd groups as compared to the 1st group. lymphocytes viability were evaluated using trypan blue, showed statistically (P≤0.05) less viable lymphocytes cells were in the 2nd group and more viable lymphocytes cells were found in the 3rd group when comparing to 1st group. Ingestion of T.vulgaris showed a protective role in preventing DNA damage according to comet assay.
 Conclusion the ethanolic T.vulgaris polyphenols extracted molecules with their unique chemical molecular structure withstand lead toxicity, could be due to chelate lead activity.

Effectiveness of Resveratrol Supplement on Some Biochemical Criteria and Bone in Lead-Exposed Rats

“…In physiological terms, lead is a toxic element even in few amounts and low concentrations (Levine et al 1989). One major effect of accumulation of lead in the cells is attributed to increasing generation of reactive oxygen species (ROS) and enhancement of lipid peroxidation of the cell membrane, mitochondrial dysfunction, DNA damage, apoptosis with depletion of antioxidant defense systems of cells (Fan et al 2020;AL-Okaily and Murad 2021;Ghazi and Al-Qaiym 2023). Furthermore, leadinduced alteration in gene expression patterns of neurotransmitters such as serotonin and dopamine (Hernández-Coro et al 2021), cause vitamin D deficiency by disrupting the conversion of vitamin D to its biological active (Rahman et al 2018), attacks the brain and central nervous system causing mental disorders and coma (Nawfal and Al-Okaily 2023).…”