Effectiveness of Resveratrol Supplement on Some Biochemical Criteria and Bone in Lead-Exposed Rats

Abstract

The present study was designed to investigate the effectiveness of resveratrol (3,5,4′-trihydroxy-trans-stilbene) supplements in alleviating the histopathological and some biochemical criteria alterations in adult female rats exposed to lead acetate. Twenty adult female Wistar rats were divided equally into four groups; Group C served as control, group G1 was gavage lead acetate (5mg/kg BW) orally, group G2 administered lead acetate (5mg/kg BW) and resveratrol supplement at dose 87mg/kg BW and group G3 received the dosage of resveratrol supplement orally as in G2. Blood samples were collected immediately before and after 28 days of the experiment to determine serum calcium ion and phosphorus concentrations. Furthermore, specimens of femur bone were taken hold at the end of the treatment. Results of serum calcium (Ca +2 ) and phosphorus showed a significant decrease in group G1 compared to other groups. Also, there were histopathological alterations in the bone sections characterized by a reduction of the cellular component of bone marrow tissue, visible empty lacunae, scattered osteocytes, and irregular roughened periosteum with increased in collagenous in the bony matrix compared to the control, G2, and G3 groups. Besides, a decrease in these parameters in group G2 was noticed compared to group G1. In conclusion, the results confirmed the effectiveness of resveratrol supplements in alleviating the adverse effects of lead acetate on calcium, and phosphorus and attenuating the histopathological changes of bone due to its antioxidant activities in rats.