Role of Surfactants in the Interaction of Dye Molecules in Natural DNA Polymers

Abstract

Solutions and powders formed from salmon sperm deoxyribonucleic acid (DNA) reacted with the cationic surfactant cetyltrimethylammonium chloride (CTMA-Cl) incorporated fluorescent rhodamine molecules: anionic sulforhodamine 640 (SRh) or cationic/zwitterionic rhodamine 640 perchlorate (RhP). The role of the cationic surfactant in the interaction between rhodamine dye and DNA-surfactant molecules has been investigated in both solution and solid state using optical spectroscopy and electrophoresis. Unexpectedly, the dye molecules did not interact directly with DNA, rather the DNA double helix acted as a template for the interaction between dye molecules and CTMA in the DNA/CTMA complex. The SRh and RhP molecules yield different fluorescence characteristics with increasing DNA/CTMA amount, indicating different configurations between the CTMA ligands.

Light amplification and lasing from dyes doped in DNA-complex thin films prepared by soaking method

“…11,13 In our case we immersed DNA-CTMA complex films into acetone solution of DMASPDB, because the complex was insoluble to acetone as well as water or DMF. Fig.…”

“…11 There are many water soluble xanthene dyes available as eosin and fluorescein as well as rhodamines, the most popular laser dyes. Eosin Y is an anionic dye and is important for staining of biological materials for microscopic observation, but it has not been studied as a laser medium.…”

“…10 You et al succeeded to stain DNA-CTMA powder by simply immersing it in aqueous solutions of dye, suggesting the importance of ion exchange process in the complex formation. 11 Being stimulated by these recent achievements, we modified their original method of sample preparation and applied it to the fabrication of laser active films containing several kinds of dyes. In this proceeding, we describe preliminary results obtained in three types of samples.…”

Electrochemistry of deoxyribonucleic acid–cetyltrimethylammonium complex with considering O2 effect

“…Biological material based on deoxyribonucleic acid-cetyltrimethylammonium complex (DNA-CTMA) has been considered as the potential candidate material for the bioelectronics and biophotonics [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. CTMA is a kind of surfactant and often used to modify DNA sodium salt, which interacted with DNA helix statically.…”

Spectral investigations on binding of DNA-CTMA complex with tetrameric copper phthalocyanines

“…While the binding of cationic porphyrins and Cu (II) phthalocyanine (Cuprolinic blue) with DNA itself has been well-investigated and the binding patterns characterized [2][3][4], little or no study has been reported with regard to their binding to DNA cationic complexes such as DNA-CTMA. While there are literature reports involving some active layers incorporating DNA-CTMA and fluorescent organic dyes such as sulforhodamine with some emphasis on their binding characteristics [5,6], the methodology described in this paper will be of general utility in the study of binding between DNA-CTMA and various organic additives of electronic/optical interest for device applications.…”