Role of short chain fatty acids in evaluation of gut microbiocenosis disorders and their correction in patients with NAFLD of different stages

Ardatskaya, M. D.; Garushyan, G. V.; Moysak, R. P.; Topchiy, T. B.

doi:10.31146/1682-8658-ecg-161-1-106-116

Cited by 5 publications

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“…Our data indicate an increase in acetic, propionic and butyric content in NAFLD patients as well as in obesity patients. Adult NAFLD patients (with a median age of 52 years) demonstrate a significant increase in fecal propionic and butyric acids content [15], which may indicate a decrease in the number and activity of obligate microorganisms such as Bifidobacterium producing acetic acid and an increase in facultative and residual anaerobic bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…The ratio of ace-tic, propionic, butyric acids is an important indicator of the integrity of the gut microbial community, which remains constant within a small interval of concentrations. The ratio of the total SCFA content to the acetic acid concentration that is called the anaerobic index (AI) allows evaluating the metabolic activity of anaerobic, indigenous gut microbiota in the total metabolic activity of all microbial tissue complex and can characterize the composition of the microbial community [15]. Nevertheless, only a few reports nowadays illustrate the role of microbiota associated with NAFLD with metabolomics data in children [16].…”

Section: Introductionmentioning

confidence: 99%

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Оценка Состояния Интестинальной Микрофлоры И Содержания Короткоцепочечных Жирных Кислот Копрофильтрата У Детей С Неалкогольной Жировой Болезнью Печени

Zavhorodnia

Lukianenko

Кленіна

et al. 2021

GASTRO

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Background. Changes in the intestinal microbiome trigger the development and progression of nonalcoholic fatty liver disease (NAFLD). Adverse fluctuations in intestinal microbiota are associated with increased intestinal permeability, activation of mucosal and adaptive immunity, increase in production and intestinal absorption of short-chain fatty acids (SCFA). The ratio of acetic, propionic, butyric acid is an important indicator of the integrity of the microbial community of the intestine. Thus, the study of the gut microbiota composition and short-chain fatty acids production represents a very appealing approach to increasing our knowledge about the mechanisms leading to NAFLD in children. The purpose of the study was to determine the features of the fecal short-chain fatty acids (fSCFA) content and the colonic microbiota composition in children with NAFLD. Materials and methods. A comprehensive examination of 102 children was provided in the Department of Pediatric Gastroenterology of the Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine. According to the presence of obesity, transient elastography data and alanine aminotransferase levels the patients were divided into four groups: I group-children with simple hepatic steatosis (n = 24); II group-children with nonalcoholic steatohepatitis (NASH) (n = 14); III group-children with obesity without steatosis (n = 48), IV group-children with normal weight (n = 16). Chromatographic study of fSCFA was conducted using gas chromatograph Chromatec-Crystal 5000. The microorganisms were identified using a microbiological study of the colon content. Diagnosis of NAFLD was established with FibroScan 502 Touch (Echosens, France) with the determination of the controlled attenuation parameter. Results. Significant changes in the spectrum of fSCFA were observed in children of the III group with acetic acid content increased by 4.8 times (р < 0.05), propionic acid by 1.5 times (р < 0.001), and butyric acid by 1.7 times as compared to the control group, while in children with NASH, acetic content was 2.5-fold increased, propionic and butyric acid-1.4-fold in comparison with the control group (p = 0.1). Also, significant anaerobic index decrease was observed in NAFLD patients. The fecal content microbiological examination demonstrated the reduced level of Bifidobacteria strains in 11.8 % patients of group I and in 8.3 % of group III; decreased levels of Lactobacillus were found in 70.6 % children of group I, in all children with NASH, in 70.8 % patients of group III. Overgrowth of bacteria such as Klebsiella was identified in 23.5 % patients of group I and in 8.3 % people of group III. Pathogenic Staphylococcus was detected in 5.9 % patients of group I, in 8.3 % patients of group III. Overgrowth of Candida was detected in 23.5 % children of group I, in 14.3 % children of group II and in 20.8 % children of group III. Conclusions. Quantitative and qualitative deviation of intestinal microbiota such as a decrease in the number of major symbionts an...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Оценка Состояния Интестинальной Микрофлоры И Содержания Короткоцепочечных Жирных Кислот Копрофильтрата У Детей С Неалкогольной Жировой Болезнью Печени

Zavhorodnia

Lukianenko

Кленіна

et al. 2021

GASTRO

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The role of short-chain fatty acids in the progression of non-alcoholic fatty liver disease

Krolevets,

Livzan,

Syrovenko

2024

Medicinskij sovet

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Introduction. Nowadays, a multifactorial model of the pathogenesis of NAFLD is recognized. It is interesting to study the contribution of changes in the composition of the intestinal microbiota and its metabolites in the development of the disease.Aim. To evaluate the contribution of research into the qualitative composition of the intestinal microbiota in relation to the risk of progression of NAFLD to reduce the loss of health- saving potential of the population.Materials and methods. An open comparative study of 83 mature-aged patients (56.6 years (46–63)) suffering from NAFLD was conducted. The levels of insulin, leptin, its receptor, adiponectin in blood serum, zonulin in feces were studied, and SCFA in feceswas determined. The analysis was carried out depending on the phenotypes of NAFLD: the degree of steatosis (1 – 40 patients, degree 2 – 18 and degree 3 – 25), the presence of NASH (43 patients), the presence of fibrosis (fibrosis was found in 35 patients). The degree of steatosis and fibrosis was assessed using elastometry. The results of the study were analyzed using the Microsoft Excel, STATISTICA 12.0 software package.Results. In patients with NAFLD, the absolute number of all SCFA in the feces was reduced. The anaerobic index was deviated towards sharply negative values (-0,711 (-0,576-(-0,830)). A high level of propionic acid was noted among the patients with fibrosis (p < 0.05). Anaerobic index, relative content of isoC4 + isoC5 + isoC6, relative content of butyric acid had a positive relationship with the St-index (rs = 0.254, rs = 0.269, rs = 0.240, p≤ 0.05). An increase in the relative amount of propionic acid was statistically significantly associated with a decrease of FLI (rs = -0.229, p ≤0.05). A positive correlation was found between the level of insulin and the absolute amount of butyric acid C4 (rs = 0.228, p ≤ 0.05). There was an inverse relationship of the absolute and relative amounts of isoC4+ isoC5 + isoC6 and Iso Cn/Cn with zonulin in the feces (rs = -0.231, p ≤ 0.05, rs = -0.380, p ≤ 0.05 and rs = -0.332, p ≤ 0.05, respectively).Conclusion. There is the anaerobic flora among the patients with NAFLD. Modification of the content of SCFA in feces may affect to the progression of NAFLD. The effect of SCFA on the development and progression of NAFLD may be mediated by the development of insulin and leptin resistance, as well as an integrity violation of the intestinal barrier.

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Clinical experience of using Polysorb in complex pathogenetic therapy of chronic hepatitis C with severe liver fibrosis

Konstantinov,

Popova,

Vasilev

et al. 2024

Medicinskij sovet

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Introduction. Violation of metabolic processes in the gastrointestinal tract in patients with chronic hepatitis C (HCV) leads to the accumulation of toxic metabolic products in the intestine, negatively affecting both the balance of the microbiota and the functional state of hepatocytes. The sorption of toxins released by opportunistic anaerobic bacteria contributes to the restoration of the population of bifidobacteria and lactobacilli, which has a beneficial effect on the functional state of the liver.Aim. To evaluate the clinical and laboratory efficacy and safety of Polysorb as part of complex pathogenetic therapy in patients with HCV with severe liver fibrosis.Materials and methods. The study included 62 patients with HCV in the stage of severe liver fibrosis (F3 according to METAVIR) of both sexes aged 18 to 65 years who were not receiving antiviral therapy. In the study group, pathogenetic therapy was supplemented with Polysorb. Clinical and laboratory parameters were evaluated before and after the course of treatment. The study of the intestinal microbiota was carried out by determining the concentration of volatile fatty acids (VFA) in the intestinal contents: acetic, propionic, butyric, the total content of isoC4 + isoC5 + isoC6 and the value of the anaerobic index by gas-liquid chromatography (chromatograph “Tsvet 100”, Russia).Results and discussion. Complex pathogenetic therapy of patients with HCV (F3), including the enterosorbent Polysorb, increases the effectiveness of treatment for clinical syndromes: right hypochondrium by 35.8%, asthenovegetative – by 13.6%, dyspeptic – from 8 to 22.5% (according to individual symptoms), cholestatic – by 8%, and also improves the biochemical parameters of liver function: p = 0.060; GGTP, p = 0.014 and it helps to stabilize the composition of the microbiota, increasing the total level of LVH (p < 0.05), mainly due to normalization of acetic acid values, improvement of the anaerobic index: before treatment -0.858 ± 0.152, after -0.601 ± 0.163 (p < 0.05).Conclusion. The inclusion of Polysorb in the pathogenetic therapy of patients with HCV (F3) helps to stabilize the composition of the microbiota, while no side effects or adverse events have been recorded.

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Role of short chain fatty acids in evaluation of gut microbiocenosis disorders and their correction in patients with NAFLD of different stages

Cited by 5 publications

References 0 publications

Оценка Состояния Интестинальной Микрофлоры И Содержания Короткоцепочечных Жирных Кислот Копрофильтрата У Детей С Неалкогольной Жировой Болезнью Печени

Оценка Состояния Интестинальной Микрофлоры И Содержания Короткоцепочечных Жирных Кислот Копрофильтрата У Детей С Неалкогольной Жировой Болезнью Печени

The role of short-chain fatty acids in the progression of non-alcoholic fatty liver disease

Clinical experience of using Polysorb in complex pathogenetic therapy of chronic hepatitis C with severe liver fibrosis

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