Role of saffron and its constituents on cancer chemoprevention

Zhang, Zhe; Wang, Chong‐Zhi; Wen, Xin; Shoyama, Yukihiro; Yuan, Chun‐Su

doi:10.3109/13880209.2013.771190

Cited by 59 publications

(30 citation statements)

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“…Constituents of saffron responsible for the colour are the yellow-red crocins, present in a quantity of ≤ 2% of the dry weight. The coloured compounds are water-insoluble mono-and diglycosides, and brickred polyenedicarbonic acid crocetin (8,8 -diapocarotin-8,8 -dicarbonic acid) (Zhang et al 2013). Traces of free crocetin (α-crocetin), methyl crocetin (monomethyl ester of α-crocetin = β-crocetin) and trans-dimethyl crocetin (γ -crocetin) can be found.…”

Section: Introductionmentioning

confidence: 98%

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Parray

Kamili

Hamid

et al. 2014

Frontiers in Life Science

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In this study, we investigated the antibacterial and antioxidant activities of Crocus sativus L. Kashmirianus c.v. extracts (callus and stigmas). Profuse callus was obtained on MS medium enriched with BAP (20 µM) + NAA(15 µM) under in vitro conditions from corm slices. Four pathogenic bacterial strains (Staphylococcus aureus CD0001, Escherichia coli CD0006, Pseudomonas aeruginosa CD0023 and Shigella flexneri CD0033) were used for determining the antibacterial activity of extracts. The antioxidant activity was determined by DPPH assay, DNA protection assay, FTC method, TBA assay and lipid peroxidation assay. The methanol stigma extract of saffron was found to be more effective in inhibiting all the pathogenic strains. The stigma extract also showed significant radical scavenging or chelation capacities in four of the methods; however, callus extract exhibited maximum inhibition of peroxy-radicals in lipid peroxidation assay. The protocol for callus production is described. It was concluded that as well as the specific parts of plants displaying diverse pharmacological activities, callus produced under in vitro conditions will assist in enhancing the production of secondary metabolites, which will reduce the pressure on natural saffron.Keywords: antioxidant activity; callus; in vitro culture; MIC; methanol; saffron; stigma Abbreviations: BAP = 6-benzyl adenine purine BHT = butylated hydroxyl toluene FTC = ferric thiocyanate MIC = minimum inhibitory concentration NAA = naphthalene acetic acid TBA = thiobarbituric acid.

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Section: Introductionmentioning

confidence: 98%

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Parray

Kamili

Hamid

et al. 2014

Frontiers in Life Science

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“…Both pathways converge to activate caspase‐3. In our researches on anticancer phytotherapy, we reported the induction of apoptosis in human cancer cell lines using natural products such as crocin from saffron (Zhang, Wang, Wen, Shoyama, & Yuan, ), alkanin from Alkanna tinctoria (Tung, Du, Yuan, Shoyama, & Wang, ), corosolic acid from Eriobotrya japonica leaves (Uto et al, ), saikosaponin from Bupleurum falcatum (Tung, Uto, Morinaga, & Shoyama, ), diarylheptanoids from Alnus japonica (Uto et al, ), coumarin derivatives from Mammea siamensis (Uto, Tung, Thongjankaew, Lhieochaiphant, & Shoyama, ), and quassinoid from Eurycoma longifolia (Tung, Nakajima, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative activity and apoptosis induction by trijuganone C isolated from the root of Salvia miltiorrhiza Bunge (Danshen)

Uto

Tung

Ohta

et al. 2018

Phytotherapy Research

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In this study, we found that the hexane fraction of Danshen, the dried root of Salvia miltiorrhiza (Lamiaceae), exerted antiproliferative effects on human leukemia cells. Phytochemical investigation of the hexane fraction achieved the isolation of the tanshinone diterpenes: dihydrotanshinone I (1), trijuganone C (2), trijuganone B (3), cryptotanshinone (4), tanshinone IIA (5), and tanshinone I (6). Compound 2 showed significant antiproliferative activities against human leukemia cells HL-60, Jurkat, and U937. The antiproliferative activities of 2 against human cancer and normal cells indicated that 2 exhibited potent antiproliferative activities with IC values less than 10 μM against HL-60 and Jurkat cells as well as on the colon cancer cells DLD-1, COLO 205, and Caco-2. Compound 2 induced chromatin condensation, DNA fragmentation, activation of caspase-3, -8, and -9, and the cleavage of poly (ADP-ribose) polymerase (PARP) in HL-60 cells. Moreover, 2 activated Bid and Bax, leading to the loss of mitochondrial membrane potential, and 2 induced the cytochrome c release from mitochondria into cytosol. In contrast, Bcl-2 and Bcl-xL were unaffected by 2. These results suggest that 2 exerts antiproliferative effects via apoptosis induction mediated by mitochondrial dysfunction and caspase activation. Compound 2 may serve as a candidate of potential chemotherapeutic agent for human leukemia.

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“…However, the detailed mechanisms that underlie the induction of apoptosis by BBR remain poorly delineated (Sun et al, 2009;Tillhon et al, 2012). Our previous studies have reported apoptosis induction in human cancer cell lines using natural products such as crocin in saffron (Zhang et al, 2013), alkanin in Alkanna tinctoria , saikosaponin in Bupleurum falcatum , quassinoid in Eurycoma longifolia (Tung et al, 2017), curcuminoid in Alnus japonica (Tung et al, 2014), corosolic acid in Eriobotrya japonica leaves, and coumarin derivatives in Mammea siamensis (Uto et al, 2016(Uto et al, , 2013.…”

Section: Introductionmentioning

confidence: 99%

Berberine Induces Apoptotic Cell Death via Activation of Caspase-3 and -8 in HL-60 Human Leukemia Cells: Nuclear Localization and Structure–Activity Relationships

et al. 2017

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Berberine (BBR), an isoquinoline alkaloid, is a well-known bioactive compound contained in medicinal plants used in traditional and folk medicines. In this study, we investigated the subcellular localization and the apoptotic mechanisms of BBR were elucidated. First, we confirmed the incorporation of BBR into the cell visually. BBR showed antiproliferative activity and promptly localized to the nucleus from 5 min to 15 min after BBR treatment in HL-60 human promyelocytic leukemia cells. Next, we examined the antiproliferative activity of BBR (1) and its biosynthetically related compounds (2-7) in HL-60 cells. BBR exerted strongest antiproliferative activity among 1-7 and the results of structures and activity relation suggested that a methylenedioxyl group in ring A, an O-alkyl group at C-9 position, and the frame of isoquinoline may be necessary for antiproliferative activity. Moreover, BBR showed the most potent antiproliferative activity in HL-60 cells among human cancer and normal cell lines tested. Next, we examined the effect of BBR on molecular events known as apoptosis induction. In HL-60 cells, BBR induced chromatin condensation and DNA fragmentation, and triggered the activation of PARP, caspase-3 and caspase-8 without the activation of caspase-9. BBR-induced DNA fragmentation was abolished by pretreatment with inhibitors against caspase-3 and caspase-8, but not against caspase-9. ERK and p38 were promptly phosphorylated after 15 min of BBR treatment, and this was correlated with time of localization to the nucleus of BBR. These resultsCorrespondence to: Prof. Yukihiro Shoyama, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan. Tel: (þ81) The American Journal of Chinese Medicine, Vol. 45, No. 7, 1497-1511 demonstrated that BBR translocated into nucleus immediately after treatments and induced apoptotic cell death by activation of caspase-3 and caspase-8.

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Role of saffron and its constituents on cancer chemoprevention

Cited by 59 publications

References 50 publications

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Antiproliferative activity and apoptosis induction by trijuganone C isolated from the root of Salvia miltiorrhiza Bunge (Danshen)

Berberine Induces Apoptotic Cell Death via Activation of Caspase-3 and -8 in HL-60 Human Leukemia Cells: Nuclear Localization and Structure–Activity Relationships

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